Antifungal Activity of Croton aromaticus L. in vitro, Against Post-Harvest Fungal Pathogens Isolated from Tropical Fruits

Abstract

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Croton argyrophyllus Kunth and Croton heliotropiifolius Kunth: Phytochemical characterization and bioactive properties

“…The antimicrobial activity of essential oils is influenced by its chemical composition and abundance of each compound (Nazzaro et al, 2013), that could present different action mechanisms at cell level. In this work, the largest component of the oil of this species is bicyclogermacrene, a sesquiterpenoid with well-known antimicrobial activity in other essential oils (Fontenelle et al, 2008;Wijesundara et al, 2016). Other compounds present in high amounts in the EO from C. argyrophyllus were spathulenol, caryophyllene, and β-pinene.…”

“…In this context, the use of plant extracts and essential oils in food and pharmaceutical industries has been T growing significantly and is an open field with great potential. Recent works have demonstrated the antimicrobial, insecticidal and antioxidant activities of Croton species (Queiroz et al, 2014;Wijesundara et al, 2016) which are correlated with the chemical composition of their essential oils. Compounds present in the essential oils of some Croton species, such as spathulenol and caryophyllene oxide, have the capacity to inhibit the growth of some filamentous fungi species (Wenqiang et al, 2006); whereas bicyclogermacrene, α-pinene and caryophyllene have been described to possess antimicrobial activity (Cavin et al, 2006;Morais et al, 2006;Ramos et al, 2013).…”

Control of Anthracnose in Banana Cultivar Kolikuttu (<em>Musa</em> sp.) with Essential Oils and Bio-safe Fruit Coatings

“…The pathogen-causing banana anthracnose was isolated from the diseased banana peel onto Potato Dextrose Agar (PDA) following the standard protocol described by Wijesundara et al (2015). Fungal species were identified based on mycelial/conidial morphology (CMI descriptions).…”

“…Pathogenicity of the causal agent was confirmed by performing Koch's Postulates. Conidia were obtained from the isolated pure culture of C. musae and a suspension was prepared (10 5 conidia/ml) to inoculate onto healthy fruits and reisolate the causal agent to confirm pathogenicity as described by Wijesundara et al (2015). The pure cultures of C. musae causing Anthracnose were maintained in the Research laboratory of the Open University of Sri Lanka under ambient conditions at 28±2 ˚C during the experiment.…”

<em>In vitro</em> study of selected essential oils against <em>Colletotrichum</em> sp. and <em>Lasiodiplodia</em> sp. causing postharvest diseases in papaya

“…After the emergence of mycelia growth, each fungal colony was transferred to fresh PDA plates and incubated at room temperature (28±2 C) for 2-4 days to obtain pure cultures. Sub-culturing was done every two weeks and maintained on PDA at 28±2 C (Wijesundara et al, 2015). Ten isolates from each fungal species of each commodity were identified in pure culture following 7-10 days incubation based on fungal / conidial morphology (CMI descriptions).…”