Antifungal activity of indigenous Bacillus spp. isolated from soil

Bjelić, Dragana; Ignjatov, Maja; Marinković, Jelena; Spremo, Nemanja; Karaman, Maja; Nikolić, Zorica; Ivanović, Žarko

doi:10.2298/zmspn1733261b

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…In our previous study, we observed very strong antifungal activity of B. subtilis B5 and B7 against F. proliferatum BL5 (KX092461) and F. oxysporum f. sp. cepae BL7 (KX092466), while the antagonistic effect of Bacillus isolates toward F. verticillioides BL7 (KX092464) was not detected (Bjelić et al, 2017).…”

Section: Resultsmentioning

confidence: 97%

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Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic

Bjelić

Ignjatov

Marinković

et al. 2018

Zemdirbyste-Agriculture

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Clove rot caused by Fusarium spp. is a very important disease of common garlic (Allium sativum L.) occuring in many areas of the world. However, there is a lack of data about biocontrol of these pathogens. Bacillus species are attractive for research due to their potential use in the biological control of fungal diseases. The aim of this study was to select effective biocontrol agents from a series of indigenous Bacillus spp. isolated from soil. Bacterial isolates positive for hydrolytic enzymes production were screened for antifungal activity against Fusarium spp. isolated from infected garlic cloves. Polymerase chain reaction (PCR) analyses were used for molecular identification of bacterial (16S rDNA gene) and fungal (EF-1α gene) isolates, and detection of biosynthetic genes for antimicrobial lipopeptides (surfactin, iturin, bacillomycin D and fengycin) in Bacillus spp. The obtained results confirmed the presence of Fusarium tricinctum, F. oxysporum f. sp. cepae, F. proliferatum, F. acuminatum and F. verticillioides as the causal agents of garlic clove rot. Four bacterial isolates identified as Bacillus subtilis exhibited the highest antagonistic effect during in vitro testing of antifungal activity (up to 71% reduction in fungal growth), and caused a significant suppression of garlic clove infection (up to 58% reduction in rot symptoms) in situ. Analysis of the antifungal compounds involved in the antagonistic activity of the examined isolates revealed their ability to produce the antibiotic lipopeptide surfactin. The most effective isolates of B. subtilis could be used as potential biocontrol agents of garlic clove rot.

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Section: Resultsmentioning

confidence: 97%

“…Bacillus spp. were isolated from soil samples collected in several locations in Serbia as described by Bjelić et al (2017). Soil samples included the rhizosphere of plants, agricultural and non-agricultural soils, and differed in their physical and chemical properties, cropping and tillage history.…”

Section: Methodsmentioning

confidence: 99%

Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic

Bjelić

Ignjatov

Marinković

et al. 2018

Zemdirbyste-Agriculture

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Production and assortment of dry beans in Serbia

Vasić¹,

Šeremešić²,

Marinković³

et al. 2021

Biljni lekar

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Dry beans belong to pulses, species from Fabaceae family. Its dry grain is used in human nutrition. It is valued as traditional and modern food and medicine, protein source, and high-energy concentrated food. It is an important crop in agricultural production and international trade of many countries, economically viable and good preceding crop. Dry beans are considered the national dish of all people from Balkans, and it is inviolable in Serbia. Crossing of important trade routes, land and climate, turbulent history, which caused frequent population migrations, led to a great divergence of bean germplasm in our area. Domestic bean populations are exceptional genetic potential for breeding of new varieties that could give satisfactory results in these environmental conditions, with the application of appropriate cultivation technology. Areas under beans in Serbia are declining. Due to short vegetation and other biological traits, bean is very intensive and sensitive crop. This is emphasized by exceptional sensitivity to polyphagous insects, as well as the presence numerous pathogens. Low average yield is affected by weather, primarily high temperatures and drought. Yields could be increased by correct and timely application of modern agronomic knowledge, irrigated cultivation, in a joint crop or by sowing as second crop.

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Antifungal activity of indigenous Bacillus spp. isolated from soil

Cited by 2 publications

References 15 publications

Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic

Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic

Production and assortment of dry beans in Serbia

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