Antifungal activity of leaf extract of Mansoa alliacea against Colletotrichum acutatum the cause of anthracnose disease on chili pepper

Sudirga, Sang Ketut; Ginantra, I Ketut; Darmayasa, Ida Bagus Gede

doi:10.1088/1755-1315/347/1/012058

Cited by 7 publications

(9 citation statements)

References 3 publications

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“…To determine the content of phytochemicals in the extracts of P. caninum, P. betle var. Nigra and P. betle, they were tested for GC-MS [20,21]. The samples were analyzed in the form of plant leaf extracts.…”

Section: Gas Chromatography-mass Spectrophotometry (Gc-ms) Analysismentioning

confidence: 99%

The Synergistic Action of Three Piper Plant Extracts and Biofertilizer for Growth Promotion and Biocontrol of Blast Disease in Red Rice

et al. 2021

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Bali is a world tourist destination and has many natural resources that need to be developed to support the tourism sector. One of the local Bali resources that has the potential to be developed to support tourism and food is the local red Bali rice. This local Balinese rice is a characteristic of the ecotourism area of the Jatiluwih village of Tabanan, Bali. Balinese rice is grown with inorganic pesticides and there is an urgent need to develop organic pesticides as a sustainable approach to rice farming. In this regard, extracts of piper plants can serve as the best and greenest biopesticides as plant growth-promoting rhizobacteria (PGPR), and compost functions as organic fertilizer. The present research aimed to evaluate PGPR, compost, and the synergistic biopesticidal effects of extracts of three piper plants, namely Piper caninum, Piper betle var. Nigra, and Piper betle, against blast disease in Bali red rice plants. The results showed that the synergistic action of PGPR, compost, and crude extract of piper plant provided an inhibitory activity against blast disease in rice plants where the greatest inhibition was found in a mixture of the three extracts with an inhibition of 50 cm. This shows that the mixed compounds of the three piper extracts work synergistically in suppressing blast disease; in addition, PGPR also exhibited a positive impact on the growth of red rice because PGPR produce growth hormones and various antifungal metabolites that help the plant growth and induce systemic resistance against phytopathogens. The active principles were identified as citronella, trans-geraniol, and 4.6-dipropyl-nonan-5-one. A combination of these extracts with compost and PGPR showed potential antifungal activity against blast disease at a concentration of 2%. This application also promoted the growth of Bali red rice. There is a significant increase in the number of leaves and the number of tillers, where the height is inversely proportional to the higher the extract up to 2%, as the height of the red Bali rice plant decreases. This is good because it reduces the red Bali rice stalks’ possibility of falling during small production. The piper extract mixture at a concentration of 2% had the highest effect on grain production/tonne (6.59 tonne/ha) compared to the control at only 3.21–3.41 tonnes/ ha. The 2% concentration of the extracts from the mixture of the three pipers has the highest effect on growth and red Bali rice production, and provides the greatest obstacle to the intensity of blast disease in red Bali rice.

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Section: Gas Chromatography-mass Spectrophotometry (Gc-ms) Analysismentioning

confidence: 99%

The Synergistic Action of Three Piper Plant Extracts and Biofertilizer for Growth Promotion and Biocontrol of Blast Disease in Red Rice

et al. 2021

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“…Pembuatan ekstrak menggunakan metode maserasi yaitu daun yang telah bersih, dikering-anginkan pada suhu ruang, kemudian diblender hingga berbentuk serbuk. Sebanyak 100 g serbuk daun dimaserasi selama 72 jam menggunakan 1 Liter metanol Pro Analysis (Sudirga et al, 2014). Maserasi pada suhu kamar selama 72 jam dengan pengadukan dua kali sehari.…”

Section: Bahan Dan Metode Pembuatan Ekstrak Kasarunclassified

“…Pengujian MIC dilakukan dengan mendepositkan sebanyak 20 µL ekstrak konsentrasi 100, 500, 1.000, dan 2.000 ppm (b/v), kontrol negatif (metanol 1.000.000 ppm) dan kontrol positif (Nystatin 50.000 ppm) pada sumur difusi. Semua perlakuan diinkubasi pada suhu ruang selama 48 jam (Sudirga et al, 2014). Zona bening yang terbentuk diukur diameternya dan hasilnya dianalisis dengan ANOVA (SPSS 23) serta diuji lanjut Duncan Multiple Range Test (signifikansi.5%).…”

Section: Uji Penghambatan Pertumbuhan Aspergillus Niger Secara In Vitrounclassified

Potential of Awar-awar Leaf Methanol Extract (Ficus septica Burm. f.) Against Aspergillus niger on Balinese Lontar

Julyeda¹,

Darmayasa²,

Hardini³

2021

metamorfosa

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Ficus septica Burm. f. merupakan tumbuhan liar yang memiliki senyawa antifungi. Eksplorasi senyawa antifungi dari bahan alam sangat penting untuk menghambat pertumbuhan Aspergillus niger, salah satu jamur perusak lontar Bali. Bahan alam digunakan untuk mengurangi kerusakan lontar akibat bahan sintetis. Penelitian ini bertujuan mengetahui kemampuan ekstrak metanol daun awar-awar dalam menghambat pertumbuhan A. niger secara in vitro. Metode penelitian menggunakan rancangan acak lengkap dengan 7 perlakukan yaitu konsentrasi 10.000, 20.000, 30.000, 40.000, 50.000 ppm (b/v), kontrol positif (nystatin), dan kontrol negatif (metanol). Uji minimum inhibitory concentration (MIC) menggunakan 6 perlakuan yaitu konsentrasi 100, 500, 1000, 2000 ppm (b/v), kontrol positif (nystatin), dan kontrol negatif (metanol). Pemberian ekstrak pada sumur difusi dengan media potato dextrose agar (PDA). Data penelitian dianalisis statistik ANOVA (DMRT taraf signifikansi 5%) dengan perangkat SPSS versi 23. Hasil penelitian menunjukkan ekstrak metanol daun awar-awar memiliki MIC pada konsentrasi 500 ppm (b/v). Konsentrasi 10.000-50.000 ppm (b/v) menunjukkan diameter zona hambat berturut-turut sebesar 20.50 mm; 23.00 mm; 23.50 mm; 23.75 mm; 24.75 mm. Konsentrasi 10.000 ppm (b/v) memiliki daya hambat yang lebih tinggi dibadingkan dengan kontrol positif. Konsentrasi 20.000 ppm (b/v) merupakan konsentrasi optimum penghambatan ekstrak metanol daun awar-awar. Simpulan dari penelitian ini adalah ekstrak metanol daun awar-awar memiliki senyawa antifungi yang menghambat pertumbuhan A. niger. Ekstrak metanol daun awar-awar dapat dijadikan alternatif untuk menghambat pertumbuhan jamur penyebab kerusakan lontar. Kata kunci: Aspergillus niger, jamur perusak, Ficus septica Burm. f.

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“…Penelitian tentang ekstrak tanaman yang menghambat pertum-buhan biomassa jamur pernah dilaporkan oleh Sudirga et al, (2014) bahwa ekstrak metanol daun awar-awar atau Ficus septica dapat menghambat pertumbuhan biomassa jamur Colletotrichum penyebab penyakit antracnosh pada tanaman cabai. Pada perlakuan 1% sampai 5% secara nyata (P<0,05%) ekstrak daun awar-awar dapat menghambat pertumbuhan biomassa jamur sebesar 39,53% sampai 99,91%.…”

Section: Menentukan Daya Hambat Ekstrak Daununclassified

UJI EFEKTIVITAS EKSTRAK ASETON DAUN KAYU MANIS (Cinnamomum burmanni Blume) TERHADAP JAMUR FUSARIUM SOLANI PENYEBAB PENYAKIT BUSUK BATANG PADA BUAH NAGA (HYLOCEREUS SP.) SECARA IN VITRO

Darmadi¹,

Ginantra²,

Joni³

2017

metamorfosa

Self Cite

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This research heads for examining the effectiveness of acetone extract of cinnamon leaf (Cinnamomum burmanni Blume) to the growth of Fusarium soloni fungus. Cinnamon leaf which was used in this research grows in Bedugul village Tabanan regency Bali province. The extract effectiveness experiment of cinnamon leaf to the fungus experiment was carried out by well difusion method in Biopesticide Laboratory of Agriculture Faculty Udayana University, by using the complete random plan consists of 6 treatments and 4 times refrains. The concentration treatment of acetone extract of cinnamon leaf obviously (P<0.05) can impede the growth of colony, biomass and establishment of experiment fungus spore i.e. Fusarium soloni in accordance with in-vitro by PDA and PDB media. Extract minimum blocked energy of cinnamon leaf in experiment fungus i.e. 0,5%. The extract of cinnamon leaf obviously can impedethe growth of colony, spore establishment and biomass establishment of experiment fungus. In extract concentration treatment 0.5% obviously can impede the colony growth of experiment fungus, spore establishment and fungus biomass establishment i.e. each of them in amount of 17,3%, 41,45% and 7,94% if they are compared by the control. The higher extract concentration then the blocked energy is the bigger.

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Antifungal activity of leaf extract of Mansoa alliacea against Colletotrichum acutatum the cause of anthracnose disease on chili pepper

Cited by 7 publications

References 3 publications

The Synergistic Action of Three Piper Plant Extracts and Biofertilizer for Growth Promotion and Biocontrol of Blast Disease in Red Rice

The Synergistic Action of Three Piper Plant Extracts and Biofertilizer for Growth Promotion and Biocontrol of Blast Disease in Red Rice

Potential of Awar-awar Leaf Methanol Extract (Ficus septica Burm. f.) Against Aspergillus niger on Balinese Lontar

UJI EFEKTIVITAS EKSTRAK ASETON DAUN KAYU MANIS (Cinnamomum burmanni Blume) TERHADAP JAMUR FUSARIUM SOLANI PENYEBAB PENYAKIT BUSUK BATANG PADA BUAH NAGA (HYLOCEREUS SP.) SECARA IN VITRO

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