Antifungal activity of oligochitosans (short chain chitosans) against some Candida species and clinical isolates of Candida albicans: Molecular weight–activity relationship

Куликов, С. М.; Lisovskaya, Svetlana А.; Zelenikhin, P. V.; Bezrodnykh, Evgeniya A.; Шакирова, Д Р; Blagodatskikh, I. V.; Тихонов, В. Е.

doi:10.1016/j.ejmech.2013.12.017

Cited by 96 publications

(52 citation statements)

References 64 publications

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“…As previously reported, the adsorption of chitosan onto phospholipid vesicles is driven mainly by electrostatic interaction and it is reinforced by the presence of negatively charged phospholipids (Quemeneur et al, 2008). Alterations of and damage to cell membranes of fungi with chitosans of low Mw against Candida species (Kulikov et al, 2014) and Neurospora crassa (Palma-Guerrero et al, 2009) have been reported. On the other hand, a fungistatic mechanism has been proposed for Aspergillus parasiticus and attributed to the high Mw (170-300 kDa) of the chitosan samples used (Seyfarth, Schliemann, Elsner, & Hipler, 2008).…”

Section: Interaction Of the Amphiphilics With Model Membranesmentioning

confidence: 67%

“…The antimicrobial activity of chitosan depends either on the type of microorganism or on molecular weight (Li, Chen, Liu, Zhang, & Tang, 2011). For instance, chitosans of low Mw showed higher antimicrobial activity against Candida species (Kulikov et al, 2014;Seyfarth et al, 2008) than those of high Mw (Tayel et al, 2010). Nevertheless, a fine-tuning on Mw is needed to achieve more effective molecules (Kulikov et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Insights on the antifungal activity of amphiphilic derivatives of diethylaminoethyl chitosan against Aspergillus flavus

Dias¹,

Cabrera

Lima³

et al. 2018

Carbohydrate Polymers

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In this study, the antifungal activity of chitosan derivatives against A. flavus was studied to understand the contribution of the molecular mass (Mw) and of the hydrophobic and electrostatic forces to the inhibition of fungal growth. The interaction of amphiphilics ranging from 8 to 130 kDa with model membranes of zwitterionic L-α-phosphatidylcholine (PC) and anionic L-α-phosphatidylcholine/L-α-phosphatidyl-DL-glycerol (PC:PG, 80:20 mol%) were exploited to obtain information on the inhibition mechanism. The results indicated that concurrent interactions control the antifungal activity. The decrease in the Mw weakens the self-association favoring the electrostatic and hydrophobic associations with the cell wall and anionic lipids of the lipid bilayer, indicating an increasing association of the amphiphilics with the fungal membrane. Laser confocal scanning microscopy of rhodamine labeled-derivatives and transmission electronic microscopy techniques showed that the amphiphilics affect the cell wall integrity by inducing the aggregation of hydrophobic constituents of the conidia.

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Section: Interaction Of the Amphiphilics With Model Membranesmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Insights on the antifungal activity of amphiphilic derivatives of diethylaminoethyl chitosan against Aspergillus flavus

Dias¹,

Cabrera

Lima³

et al. 2018

Carbohydrate Polymers

View full text Add to dashboard Cite

show abstract

“…2 Chitosan inhibits growth of filamentous fungi and yeast human pathogens. 3,4 To develop chitosan as an antifungal treatment, a full understanding of its mode of action is necessary. In Saccharomyces cerevisiae, the response to chitooligosaccharides is mediated by proteins associated with plasma membrane, respiration, ATP production and mitochondrial organization.…”

Section: Introductionmentioning

confidence: 99%

Neurospora crassa transcriptomics reveals oxidative stress and plasma membrane homeostasis biology genes as key targets in response to chitosan

et al. 2016

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Chitosan is a natural polymer with antimicrobial activity. Chitosan causes plasma membrane permeabilization and induction of intracellular reactive oxygen species (ROS) in Neurospora crassa. We have determined the transcriptional profile of N. crassa to chitosan and identified the main gene targets involved in the cellular response to this compound. Global network analyses showed membrane, transport and oxidoreductase activity as key nodes affected by chitosan. Activation of oxidative metabolism indicates the importance of ROS and cell energy together with plasma membrane homeostasis in N. crassa response to chitosan. Deletion strain analysis of chitosan susceptibility pointed, NCU03639 encoding a class 3 lipase, involved in plasma membrane repair by lipid replacement and NCU04537 a MFS monosaccharide transporter related with assimilation of simple sugars, as main gene targets of chitosan. NCU10521, a glutathione S-transferase-4 involved in the generation of reducing power for scavenging intracellular ROS is also a determinant chitosan gene target. Ca2+ increased tolerance to chitosan in N. crassa. Growth of NCU10610 (fig 1 domain) and SYT1 (a synaptotagmin) deletion strains was significantly increased by Ca2+ in presence of chitosan. Both genes play a determinant role in N. crassa membrane homeostasis. Our results are of paramount importance for developing chitosan as antifungal.

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“…16,25 Many chitosan derivatives exist, and their properties and antimicrobial activity vary with the molecular weight, degree of deacetylation, pH, and other factors. [26][27][28][29][30] Therefore, several chitosan derivatives with different chemical characteristics should be examined to determine those suitable for each application.…”

mentioning

confidence: 99%

Activity of chitosan antifungal denture adhesive against common Candida species and Candida albicans adherence on denture base acrylic resin

Namangkalakul

Benjavongkulchai²,

Pochana

et al. 2020

The Journal of Prosthetic Dentistry

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Antifungal activity of oligochitosans (short chain chitosans) against some Candida species and clinical isolates of Candida albicans: Molecular weight–activity relationship

Cited by 96 publications

References 64 publications

Insights on the antifungal activity of amphiphilic derivatives of diethylaminoethyl chitosan against Aspergillus flavus

Insights on the antifungal activity of amphiphilic derivatives of diethylaminoethyl chitosan against Aspergillus flavus

Neurospora crassa transcriptomics reveals oxidative stress and plasma membrane homeostasis biology genes as key targets in response to chitosan

Activity of chitosan antifungal denture adhesive against common Candida species and Candida albicans adherence on denture base acrylic resin

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