Antifungal activity of Terminalia superba (combretaceae)

Abstract

KEYWORDS Terminalia superb Anticandidosic Ellagic acid derivative ABSTRACTThe aim of the present study was to optimize the anticandidosic activities of Terminalia superba (TEKAM4) and the identification of major compounds present in the most active chromatographic fraction. The hydroethanolic extract TEKAM4-X0 was prepared by homogenization employing a blender. Two derivatives extracts of TEKAM4-X0 (X1-1 and X1-2) were obtained by a liquid/liquid partition of TEKAM4-X0 in a mixture of hexane and water (v/v). Three chromatographic fractions (F1, F2 and F3) from X1-2 were separated by means of Sephadex-LH20 gel filtration chromatography. All the extracts were incorporated to Sabouraud according to the agar slanted double dilution method. Ketoconazole was used as standards for antifungal assay. The entire fractions were tested on the previously prepared medium culture containing 1000 cells of C. albicans. Antifungal activity was determined by evaluating antifungal parameters values (MFC and IC50). Lastly, the structures of 2 isolated compounds were elucidated by combination of Flash chromatography and spectroscopic methods, including MS, and multiple stage RMN experiments.

Etude ethnobotanique de plantes antifongiques utilisées traditionnellement en Côte d’Ivoire et du potentiel de <i>Piliostigma Thonningii</i> (schumach.) milneredh. (Fabaceae) dans le contrôle de souches telluriques

“…Les milieux ont été stérilisés à l'autoclave à 121 °C pendant 15 minutes. L'incorporation des différents extraits végétaux au milieu de culture a été faite selon la méthode de la double dilution de liaison géométrique de raison ½ (Zirihi et al, 2003 ;Ahon et al, 2011). Les différentes concentrations utilisées ont été : 100 ; 50 ; 25 ; 12,5 ; 6,25 ; 3,12 et 1,56 mg/ml.…”