Aim:This study was to evaluate in vitro anti-fungal activity of aqueous and hydroethanolic from medicinal plants extracts collected in Côte d’Ivoire.Materials and Methods:Plants extracts were prepared by homogenization and separately incorporated to Sabouraud agar using the agar slanted double dilution method. Ketoconazole was used as standards for anti-fungal assay. The anti-fungal tests were performed by sowing 1000 cells of Candida albicans on the previously prepared medium culture. Anti-fungal activity was determined by evaluating anti-fungal parameters values (minimal fungicidal concentrations [MFC] and IC50).Results:The results showed that all extracts possessed anti-fungal activities whose levels vary from plant species to another. Eight of them had a satisfactory anti-candidosic activity and extracts from Terminalia species were the most active. Among them the Terminalia superba extracts generated the strongest activities (MFC = 0.0975 mg/mL). Compared with ketoconazole (MFC = 0.390 mg/mL), the T. superba extracts, aqueous (MFC = 0.195 mg/mL) and hydroethanolic (0.0975 mg/mL) were successively twice and four times more active. The worst anti-fungal activity (MFC = 1600 mg/mL) was obtained with the Guarea cedrata aqueous extract.Conclusion:All medicinal plants extracts produced anti-fungal activities, and T. superba was the most active.

Aims: In traditional medicine, several plant species from Rutaceae’s family have been used to treat sickle cell anemia. However, more studies are needed to corroborate the antisickling activity of Zanthoxylum leprieurii species. The objective of this paper was to evaluate the sickling cell inhibition and radical scavenging activities of hydroethanolic and aqueous extracts of Zanthoxylum leprieurii’s bark, a plant species used in the management of sickle cell anemia in eastern Côte d’Ivoire. Methods: Hydroethanolic and aqueous extracts of the stem bark of Zanthoxylum leprieurii were prepared. Qualitative and quantitative phytochemical tests were carried out. In addition, diphenyl 1, 2 picrylhydrazyl (DPPH) was used to determine the antioxidant potential of these two extracts. The antisickling activity of two extracts was determined by the Emmel method. Results and Discussion: Zanthoxylum leprieurii’s barkextracts have demonstrated antioxidant property. The IC50 value of the hydroethanolic extract (0.308±0.06) was lower than that of the decocted (0.434 ±0.06). At 10 mg/mL, the sickling cell inhibition of DZL was 81% ±2.66 while EZL was 89% ±0.44. The presence of alkaloids, sterols, Polyterpenes and phenolic compounds in both extracts could explain the sickling inhibition activity of these extracts. All the extracts revealed an antioxidant and antisickling activities higher than the standard. Conclusion: The hydroethanolic extract (EZL) demonstrated a higher antisickling activity and exhibited a better free radical scavenging activity. The use of Zanthoxylum leprieurii’s bark in the traditional management of sickle cell anemia is justified.

An ethnobotanical survey was conducted to record the various plant families, species, and plant parts used to manage sickle cell disease in the Indenié-Djouablin region eastern Côte d’Ivoire. Nine traditional healers aged 36 to 67 years old were selected in six different villages according to their reputation in knowledge of traditional medicine, their availability and their willingness to share information. A total of 26 species of plants belonging to 20 families were reported to have antisickling activity. Eleven (11) different medication recipes were composed from these 26 species of plants ((Afromonum melegueta (Roscoe) K. Schum., Xylopia aethiopica) mainly). The main plant parts used were stem bark (38%) followed by fruit (18%) and seeds (18%). The majority of the main plants recorded (84.61 %) were wild. From the recorded plants, 65.38 % were trees and 23.07 % shrubs. Most remedies (82%) were made up by decoction and were employed orally (54 %) or by enema (32 %). The potential anti-sickling activity of the plant species recorded during this study could be the effect of alkaloids, polyphenols, quinones and terpenoids compounds found during the phytochemical screening of the 26 plants species.

KEYWORDS Terminalia superb Anticandidosic Ellagic acid derivative ABSTRACTThe aim of the present study was to optimize the anticandidosic activities of Terminalia superba (TEKAM4) and the identification of major compounds present in the most active chromatographic fraction. The hydroethanolic extract TEKAM4-X0 was prepared by homogenization employing a blender. Two derivatives extracts of TEKAM4-X0 (X1-1 and X1-2) were obtained by a liquid/liquid partition of TEKAM4-X0 in a mixture of hexane and water (v/v). Three chromatographic fractions (F1, F2 and F3) from X1-2 were separated by means of Sephadex-LH20 gel filtration chromatography. All the extracts were incorporated to Sabouraud according to the agar slanted double dilution method. Ketoconazole was used as standards for antifungal assay. The entire fractions were tested on the previously prepared medium culture containing 1000 cells of C. albicans. Antifungal activity was determined by evaluating antifungal parameters values (MFC and IC50). Lastly, the structures of 2 isolated compounds were elucidated by combination of Flash chromatography and spectroscopic methods, including MS, and multiple stage RMN experiments.

No abstract