Antifungal activity of the ethanolic extracts of Punica granatum L. and evaluation of the morphological and structural modifications of its compounds upon the cells of Candida spp.

Abstract

Ethanolic crude extracts prepared from the arils and seeds, pericarp, peels and from the whole fruit of Punica granatum, known as pomegranate, had their antifungal activity tested against Candida spp. The ethanolic crude extracts were analyzed by Mass Spectrometry and yielded many compounds such as punicalagin and galladydilacton. The extracts from the pericarp and peel showed activity against Candida spp., with MICs of 125 μg/mL. The effect of pericarp and peel extracts upon the morphological and structure of C. albicans and C. krusei were examined by scanning and transmission electron microscopy, with the visualization of an irregular membrane and hyphae, formation of vacuoles and thickening of the cell wall. The data obtained revealed potential antimicrobial activity against yeasts cells of the Candida genus, and the bioactive compounds could be responsible for changes in cell morphology and structure. The data obtained open new perspectives for future research in continuation to this study, where information such as determination of the site of action of the compounds could contribute to an alternative therapy against these organisms.

Antimicrobial activity of pomegranate peel extracts as affected by cultivar

“…Several studies have reported the effectiveness of pomegranate extracts against phytopathogenic fungi that cause major economic losses of important crops . Also, some Candida species, which are prevalent and important pathogens detected in hospitalized patients with nosocomial infections, have been treated with ethanolic extracts obtained from arils and seeds, pericarp, and peels, as well as from the whole fruit of pomegranate . Some studies have reported significant inhibitory activity against Candida albicans using pomegranate extracts …”

“…6 -11 Also, some Candida species, which are prevalent and important pathogens detected in hospitalized patients with nosocomial infections, 12 have been treated with ethanolic extracts obtained from arils and seeds, pericarp, and peels, as well as from the whole fruit of pomegranate. 13 Some studies have reported significant inhibitory activity against Candida albicans using pomegranate extracts. 14 -17 Currently, consumers are more concerned about the safety of the synthetic preservatives of food, and research has evaluated alternative natural products against microorganisms that cause food-borne illnesses.…”

Gambaran eGFR Menurut CKD-EPI pada Penderita Thalassemia Mayor di Rumah Sakit Dr. Hasan Sadikin Bandung

“…10 Suspensi C. albicans yang setara dengan kekeruhan 0,5 Mc Farland diambil sebanyak 0,1 mL dan ditanam menggunakan cotton bud pada lempeng agar. Sebanyak 5 buah cakram kertas berdiameter 8 mm ditetesi 50 μL fraksi air umbi sarang semut dengan konsentrasi 10.000 μg/mL, 5.000 μg/mL, dan 2.500 μg/mL pada cakram kertas pertama, kedua, dan ketiga, kemudian diteteskan 50 μL nistatin dengan konsentrasi 50 μg/mL ke dalam cakram kertas keempat sebagai kontrol atau pembanding.…”

The chemical composition and inhibitory action of Punica granatum (L.) extracts against Candida spp.: A brief review

“…Monitoring the ethanolic extracts from P. granatum by mass spectrometry, Anibal et al (2013) detected various compounds, among them delfinidin 3-O-glucoside, punicacortein A, punicacortein B, pedunculagin, tellimagrandin, glucose, citric acid, caffeic acid, punicic acid (cis-9, trans-11, cis-13 octadecatrienoic acid), ellagic acid, quercetin, delphinidin, gallagyldilacton and punicalagin from pericarp; and punicacortein A, punicacortein B, corilagin, glucose, citric acid, caffeic acid, punicic acid (cis-9, trans-11, cis-13 octadecatrienoic acid), pedunculagin, tellimagrandin and punicalagin from the peels.…”

“…Anibal (2007) evaluated the activity of the pomegranate through crude extracts dichloromethane and methanolic, against standard strains of C. albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis, C. dubliniensis, C. utilis, C. guilliermondii, C. lusitaniae, and C. rugosa, demonstrating antifungal activity of this plant of interest for alternative therapy against these microorganisms. Anibal et al (2013) showed that ethanolic extracts obtained from pericarp and peel presented potential inhibitory activity, with a minimun inhibitory concentration of 125 µg/ml. These data indicate that the activity of P. granatum probably occurs in the compounds present in the pericarp and peel of this fruit.…”