Antifungal activity of ε-poly-L-lysine on Trichothecium roseum in vitro and its mechanisms

Wei, Meilin; Ge, Yonghong; Li, Canying; Chen, Yanru; Wang, Wenhui; Duan, Bin; Li, Xue

doi:10.1016/j.pmpp.2018.04.005

Cited by 33 publications

(18 citation statements)

References 24 publications

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“…The previous reports showed that mycelial growth, spore germination rate, and germ-tube length of eukaryotic microorganisms (Penicillium digitatum) were markedly inhibited by ε-PL (Liu et al 2017). Furthermore, ε-PL also damaged the cell wall and cell membrane of eukaryotic microorganisms (Trichothecium roseum) (Wei et al 2018). Therefore, these results suggested that inhibition of mycelia and spore growth, and damage of the cell wall and membrane by ε-PL were closely related to the eukaryotic microorganisms' death.…”

Section: Proposed Mechanism Of Action Of ε-Pl On S Aureusmentioning

confidence: 98%

“…In the past few years, the antimicrobial activities of ε-PL have been reported (Geornaras et al 2007;Liu et al 2017). However, its antibacterial mechanism has not been absolutely elucidated (Wei et al 2018;Bo et al 2015;Hyldgaard et al 2014). Only a few reports showed that ε-PL can cause morphological damages strip the outer membrane, and cause abnormal distribution of the cytoplasm.…”

Section: Introductionmentioning

confidence: 99%

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The antimicrobial effects and mechanism of ε-poly-lysine against Staphylococcus aureus

Tan

Shi

Xing

et al. 2019

Bioresour. Bioprocess.

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Background: As a natural antibacterial cationic peptide, ε-poly-l-lysine (ε-PL) is applied as a food preservative. However, the mechanism of ε-PL against Staphylococcus aureus (S. aureus) has not been elucidated. Especially, its antimicrobial mechanism at the metabolomics has not been yet thoroughly described. Results: This work aimed at clarifying the antibacterial activity and mechanism of ε-PL against S. aureus. Effects of ε-PL with different concentration on cell morphology, cell wall, and membrane integrity were investigated. Furthermore, the effect of ε-PL on metabolite properties of S. aureus was also studied. The results revealed that ε-PL disrupted the cell wall and membrane integrity of treated cells. ε-PL induced the structural change of peptidoglycan in cell wall, causing cell wall more fragile. Meanwhile, the permeability of the S. aureus cell membrane was increased by ε-PL. More importantly, ε-PL with different concentration could cause different effects on metabolic pathways of S. aureus. ε-PL with high concentration could directly restrain the central carbon metabolism. However, ε-PL with low concentration could only inhibit the glycolytic pathway. Conclusion: These results showed that the antimicrobial mechanism of ε-PL against S. aureus was a synergistic action.

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Section: Proposed Mechanism Of Action Of ε-Pl On S Aureusmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

The antimicrobial effects and mechanism of ε-poly-lysine against Staphylococcus aureus

Tan

Shi

Xing

et al. 2019

Bioresour. Bioprocess.

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“…Multiple reports have described that ε-PL inhibits the growth of various microorganisms by affecting the integrity and permeability of their cell walls and cell membranes ( Hyldgaard et al, 2014 ; Wei M. et al, 2018 ; Lan et al, 2019 ; Su et al, 2019 ; Liu H. et al, 2020 ; Dou et al, 2021 ). For example, in Escherichia coli O157:H7 ( Figure 6A ), the interactions between ε-PL and phospholipid groups result in distorting and shriveling of the cell membrane in a non-specific, carpet-like mechanism, in which the membrane is forced to fold in on itself, contributing to vesicle/micelle and toroidal pore formation ( Hyldgaard et al, 2014 ).…”

Section: Recent Advances In Applications Of ε-Plmentioning

confidence: 99%

Epsilon-poly-L-lysine: Recent Advances in Biomanufacturing and Applications

Wang

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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ε-poly-L-lysine (ε-PL) is a naturally occurring poly(amino acid) of varying polymerization degree, which possesses excellent antimicrobial activity and has been widely used in food and pharmaceutical industries. To provide new perspectives from recent advances, this review compares several conventional and advanced strategies for the discovery of wild strains and development of high-producing strains, including isolation and culture-based traditional methods as well as genome mining and directed evolution. We also summarize process engineering approaches for improving production, including optimization of environmental conditions and utilization of industrial waste. Then, efficient downstream purification methods are described, including their drawbacks, followed by the brief introductions of proposed antimicrobial mechanisms of ε-PL and its recent applications. Finally, we discuss persistent challenges and future perspectives for the commercialization of ε-PL.

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“…This non-toxicity and good antibacterial activity has led ε-PL to be generally recognized as safe as a food preservative for rice, vegetables, fruit, soft drinks, fish, and other products in many countries, including the United States, Korea, Japan, and China [ 8 , 9 ]. Mechanistically, ε-PL is believed to invoke electrostatic interactions that damage the cell membrane, ultimately disintegrating the microbial cells [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against Botryris cinerea

Lü

et al. 2022

Microorganisms

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This study mainly evaluated the broad-spectrum fungicidal activity of ε-poly L lysine (ε-PL) against 12 pathogenic fungi. We further demonstrated synergistic antifungal activity of ε-PL combined with wuyiencin against Botryris cinerea. The combined bio-fungicide achieved an inhibition rate of 100% for mycelial growth using ε-PL at 500 μg/mL + wuyiencin at 50 μg/mL and for spore germination using ε-PL at 200 μg/mL + wuyiencin at 80 μg/mL in vitro. This synergistic spore and mycelia-damaging effect of the combination was confirmed using scanning electron microscopy. In vivo assays with combined bio-fungicide (1500 μg/mL ε-PL + 60 μg/mL wuyiencin) on detached leaves showed depressed growth and development of the spores of B. cinerea. The synergistic effect was further tested in combinations of ε-PL with wuyiencin by measuring the fractional inhibition concentration index (FICI) value below 0.5. Moreover, ε-PL and wuyiencin inoculation before B. cinerea infection significantly increased the superoxide dismutase, peroxidase, catalase, and phenylalanine ammonia-lyase activities, which suggested their involvement in tomato defense responses to disease to minimize damage to B. cinerea. These findings revealed that a combined bio-fungicide comprising ε-PL and wuyiencin had a good prospect for controlling plant fungal disease.

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Antifungal activity of ε-poly-L-lysine on Trichothecium roseum in vitro and its mechanisms

Cited by 33 publications

References 24 publications

The antimicrobial effects and mechanism of ε-poly-lysine against Staphylococcus aureus

The antimicrobial effects and mechanism of ε-poly-lysine against Staphylococcus aureus

Epsilon-poly-L-lysine: Recent Advances in Biomanufacturing and Applications

Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against Botryris cinerea

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