Antifungal and Antiaflatoxigenic Activities of 1,8-Cineole and t-Cinnamaldehyde on Aspergillus flavus

Abstract

Aspergillus flavus and A. parsiticus produce aflatoxins that are highly toxic to mammals and birds. In this study, the inhibitory effects of 1,8-cineole and t-cinnamaldehyde were examined on the growth of Aspergillus flavus ATCC 22546 and aflatoxin production. 1,8-Cineole showed 50% inhibition of fungal growth at a concentration of 250 ppm, while t-cinnamaldehyde almost completely inhibited fungal growth at a concentration of 50 ppm. Furthermore, no fungal growth was observed when the growth medium was treated with 100 ppm t-cinnamaldehyde. 1,8-Cineole also exhibited 50% inhibition on the production of aflatoxin B1 and aflatoxin B2 at a concentration of 100 ppm, while the addition of 100 ppm t-cinnamaldehyde completely inhibited aflatoxin production. These antiaflatoxigenic activities were related to a dramatic downregulation of the expression of aflE and aflL by 1,8-cineole, but the mode of action for t-cinnamaldehyde was unclear. Collectively, our results suggest that both of the compounds are promising alternatives to the currently used disinfectant, propionic acid, for food and feedstuff preservation.

Seasonal and Circadian Rhythm of a 1,8-Cineole Chemotype Essential Oil of Calycolpus goetheanus From Marajó Island, Brazilian Amazon

“…16 Moreover, 1,8-cineole has displayed antifungal and antiaflatoxigenic activities against Aspergillus flavus, at a concentration of 250 ppm with dramatic downregulation of aflE and aflL gene expression. 17 A minimum lethal dose of 50 mg/kg for a human adult has been proposed, taking into account the toxic effects of 1,8-cineole. 18…”

Can Eucalyptol Replace Antibiotics?

“…This enzyme plays a key role in the separation of the AFB1 subtype from the AFB2 subtype. As a result of the action of 1,8-cineole, the biosynthesis of AFB1 will be blocked, and the biosynthesis of AFB2 will be enhanced [ 60 ].…”

Effect of exogenous melatonin treatment onAspergillusdecay, aflatoxin B1 accumulation and nutritional quality of fresh “Akbari” pistachio fruit

“…Triggering ROS scavenging gene expression and enzyme activity in maize kernels in response to A. flavus may be effective for suppressing AB1 biosynthesis by attenuating oxidative stress representing by lower MDA content (Zhao et al., 2018). Also, cinnamaldehyde exhibits antiaflatoxigenic activity by attenuating ROS accumulation by promoting SOD/CAT enzyme activity giving rise to lower MDA content (Kim et al., 2018; Sun et al., 2016). Accordingly, attenuating Aspergillus decay and suppressing AB1 biosynthesis in fresh pistachio fruits, inoculated with A. flavus and stored at 25°C for 18 days could be ascribed to attenuating oxidative stress by promoting ROS scavenging systems activity.…”

“…Triggering ROS scavenging gene expression and enzyme activity in maize kernels in response to A. flavus may also effectual for suppressing AB1 biosynthesis by attenuating oxidative stress representing by lower MDA content (Zhao et al., 2018). Also, cinnamaldehyde exhibits antiaflatoxigenic activity by attenuating ROS accumulation by promoting activities of SOD/CAT enzymes giving rise to lower MDA content (Kim et al., 2018; Sun et al., 2016). Melatonin treatment or endogenous melatonin accumulation have been employed as efficacy strategy for triggering ROS scavenging activity, in addition to melatonin, ROS scavenging cascade (García et al., 2014; Reiter et al., 2014), which are crucial for keeping membrane integrity representing by lower MDA content (Galano & Reiter, 2018; Tan et al., 2013).…”