Antifungal, Anti-Inflammatory and Cytotoxic Effects of &lt;i&gt;Zingiber cassumunar&lt;/i&gt; Gel

Thaweboon, Sroisiri; Thaweboon, Boonyanit; Kaypetch, Rattiporn

doi:10.4028/www.scientific.net/kem.773.360

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…In the most interest of the anti-inflammatory activity, attempts have recently been made to derive this cassumunar ginger oil to formulate the tropical preparations for local and systemic administrations. The topical and the transdermal deliveries of cassumunar ginger oil have been successfully developed by microemulsion, gels and patches [6,[22][23][24]. Development of pharmaceutical products from cassumunar ginger oil are extensively growing, while the research on their toxicity is still limited.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical and Safety Evaluations of Volatile Terpenoids from Zingiber cassumunar Roxb. on Mature Carp Peripheral Blood Mononuclear Cells and Embryonic Zebrafish

et al. 2020

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Pharmaceutical products of essential oil from Zingiber cassumunar Roxb. are extensively being developed, while the research on their safety is seldom documented. The aim of the present study was to evaluate the phytochemical profile and the effect of cassumunar ginger oil on cell-based assay and the zebrafish model. The essential oil was isolated from fresh rhizomes of Z. cassumunar using simultaneous steam-distillation. Chemical composition was analyzed using gas chromatograph coupled to a mass spectrometer (GC-MS). Effect of cassumunar ginger oil on adult carp fish peripheral blood mononuclear cells (PBMCs) was investigated using MTT assay. The embryotoxic and teratogenic effects of cassumunar ginger oil were studied in zebrafish embryos. GC-MS results showed that the essential oil was composed of sabinene (43.54%) and terpinen-4-ol (29.52%) as the major phytoconstituents. No fish PBMC cytotoxic effect was observed with the concentration less than 50 µg/mL of cassumunar ginger oil. Our results showed for the first time the embryotoxic and teratogenic effects of cassumunar ginger oil in zebrafish embryos. The result indicated that the cassumunar ginger oil induced zebrafish embryotoxicity in a concentration-dependent manner. At 500 µg/mL of cassumunar ginger oil demonstrated significantly moderated embryotoxicity within 24 h (p < 0.05). The survival rate of 100 µg/mL of cassumunar ginger group was markedly declined to zero at 96-h post-fertilization (log-rank test, p = 0.001). However, survival rates of zebrafish embryo in the 1 and 10 µg/mL cassumunar ginger groups were more than 90% throughout the trial period. Moreover, very low teratogenicity to the zebrafish embryo was also observed in 1 and 10 µg/mL of cassumunar ginger groups. Our findings suggest that there is hardly any cytotoxicity, embryotoxicity and teratogenicity at concentrations less than 10 µg/mL of cassumunar ginger oil. However, the toxicity assessment of its pharmaceutical product should prove for further consumer protection.

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Section: Discussionmentioning

confidence: 99%

Phytochemical and Safety Evaluations of Volatile Terpenoids from Zingiber cassumunar Roxb. on Mature Carp Peripheral Blood Mononuclear Cells and Embryonic Zebrafish

et al. 2020

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“…Menurut Thaweboon et al (2018) pemberian minyak atsiri rimpang bangle secara topikal mampu memberikan penghambatan inflamasi yang lebih tinggi dari pada triamcinolone pada waktu 30 dan 60 menit, sedangkan penelitian minyak atsiri rimpang bangle (Zingiber purpureum Roxb.) terhadap aktivitas antikanker payudara belum dilakukan, oleh karena itu diperlukan upaya pengembangan obat dari minyak atsiri rimpang bangle sebagai antikanker payudara.…”

Section: Pendahuluanunclassified

Studi In Silico Senyawa 1,4-Naphthalenedione-2-Ethyl-3-Hydroxy sebagai Antiinflamasi dan Antikanker Payudara

et al. 2021

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Inflamasi merupakan suatu respon dari tubuh terhadap adanya cedera maupun infeksi yang ditandai dengan timbulnya kemerahan, demam, bengkak, nyeri dan hilangnya fungsi. Inflamasi berkontribusi terhadap ketidakseimbangan sekresi sitokin yang akan menghambat terjadinya apoptosis pada sel kanker sehingga menyebabkan hiperproliferasi sel. Kanker payudara merupakan salah satu penyakit kanker dengan prevalensi tertinggi di urutan ke dua di dunia. Penelitian terdahulu melaporkan pemberian minyak atsiri rimpang bangle (Zingiber purpureum Roxb.) secara topikal mampu memberikan penghambatan inflamasi yang lebih tinggi daripada triamcinolone, namun spesifik senyawa yang berpotensinya belum diketahui. Tujuan dari penelitian ini, yakni mencari senyawa aktif hasil analisis GCMS minyak atsiri rimpang bangle yang berpotensi sebagai antiinflamasi dan antikanker payudara secara in silico. Metode yang digunakan berupa screening Lipinski’s rule of Five, farmakokinetika dan toksisitas senyawa hasil analisis GC-MS, serta penambatan molekul dan dinamika molekular. Hasil screening dan simulasi penambatan molekul menunjukkan bahwa senyawa 1,4-naphthalenedione-2-ethyl-3-hydroxy dapat berikatan dengan reseptor COX-1 (antiinflamasi), dan hERα (antikanker payudara), namun lebih selektif terhadap reseptor COX-1 dengan nilai energi bebas (ΔG) yang lebih kecil yakni sebesar -7,20 kkal/mol, dibandingkan dengan interaksinya terhadap reseptor Erα yang bernilai -6,00 kkal/mol. Hasil simulasi dinamika molekular menggunakan metode kalkulasi MM-GBSA menunjukkan bahwa kompleks (1,4-naphthalenedione-2-ethyl-3-hydroxy)-(COX-1) memiliki nilai ∆GTOTAL sebesar -24,22 kkal/mol. Nilai ini lebih kecil dibandingkan dengan ∆GTOTAL kompleks (1,4-naphthalenedione-2-ethyl-3-hydroxy)-(hErα) sebesar -8,92 kkal/mol). Hal ini menunjukkan bahwa tingkat afinitas 1,4-naphthalenedione-2-ethyl-3-hydroxy terhadap COX-1 diprediksi lebih baik dan lebih poten sebagai antiinflamasi dibandingkan sebagai antikanker payudara.In Silico Study of 1,4-Naphthalenedione-2-Ethyl-3-Hydroxy Compounds as Anti-inflamation dan Breast Anticancer. Inflammation is a response from the body to injury or infection which is characterized by redness, fever, swelling, pain, and loss of function. Inflammation contributes to the imbalance of cytokine secretion which will inhibit apoptosis in cancer cells, causing cell hyperproliferation. Breast cancer is one of the cancer diseases with the second-highest prevalence in the world. The pioneering works reported that topical application of Bangle (Zingiber purpureum R) was able to provide a higher inhibition of inflammation than triamcinolone, however, the specific potential of the compound was unknown. The purpose of this study is to find active compounds that have the potential to be anti-inflammatory and anti-cancer in the breast using in silico approach. The methods used are screening Lipinski’s Rule of Five, pharmacokinetics and toxicity of compounds from GC-MS analysis and molecular docking, and molecular dynamics. The screening and molecular docking simulation results showed that the compound 1,4-naphthalenedione-2-ethyl-3-hydroxy can bind to COX-1 (anti-inflammatory), and ERα (Estrogen Reseptor α), but was more selective towards COX-1 receptor with a binding affinity (ΔG) -7.20 kcal/mol, compare to its interaction with ERα which is -6.00 kcal/mol. The results of molecular dynamics simulation using the MM-GBSA calculation method show that the complex (1,4-naphthalenedione-2-ethyl-3-hydroxy)-(COX-1) has a value of ∆GTOTAL of -24.22 kcal/mol). This value is smaller than ∆GTOTAL of the complex (1,4-naphthalenedione-2-ethyl-3-hydroxy)-(hErα) of -8.92 kcal/mol. The results indicate that the affinity level of 1,4-naphthalenedione-2-ethyl-3-hydroxy to COX-1 was predicted to be better and more potent as an anti-inflammatory than as an anti-breast cancer.

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“…Berdasarkan penelitian terdahulu, pemberian minyak atsiri rimpang bangle (Zingiber purpureum Roxb.) secara topikal mampu memberikan penghambatan inflamasi yang lebih tinggi dari pada triamcinolone pada waktu 30 dan 60 menit (Thaweboon, 2018). Penentuan aktivitas selain secara in vivo bisa juga dilakukan secara in silico.…”

Section: Pendahuluanunclassified

UJI AKTIVITAS ANTIINFLAMASI SENYAWA DALAM MINYAK ATSIRI RIMPANG BANGLE (Zingiber purpureum Roxb) SECARA IN SILICO

Bachtiar¹,

Susanti²,

Mardianingrum³

2021

J. of Pharm.

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Inflammation is a response from the body to injury or infection which is characterized by redness (rubor), increased temperature (calor), swelling (tumor), pain (dolor) and loss of function (functio laesa). The purpose of this study is to find active compounds of Bangle (Zingiber purpureum R) that have the potential to be antiinflammatory using the In Silico approach. The methods used are molecular docking and molecular dynamics.. The screening and molecular docking simulation results showed that the compound phenylmethylene can bind and have a stable interaction with the COX-1 receptor (anti-inflammatory) with binding affinity (ΔG) -6.94 kcal/ mol. This showed that the affinity level of phenylmethylene to COX-1 was predicted to be activity antiinflammatory.

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Antifungal, Anti-Inflammatory and Cytotoxic Effects of <i>Zingiber cassumunar</i> Gel

Cited by 4 publications

References 14 publications

Phytochemical and Safety Evaluations of Volatile Terpenoids from Zingiber cassumunar Roxb. on Mature Carp Peripheral Blood Mononuclear Cells and Embryonic Zebrafish

Phytochemical and Safety Evaluations of Volatile Terpenoids from Zingiber cassumunar Roxb. on Mature Carp Peripheral Blood Mononuclear Cells and Embryonic Zebrafish

Studi In Silico Senyawa 1,4-Naphthalenedione-2-Ethyl-3-Hydroxy sebagai Antiinflamasi dan Antikanker Payudara

UJI AKTIVITAS ANTIINFLAMASI SENYAWA DALAM MINYAK ATSIRI RIMPANG BANGLE (Zingiber purpureum Roxb) SECARA IN SILICO

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