Antifungal, antioxidant and cytotoxic activities of chitosan nanoparticles and its use as an edible coating on vegetables

Divya, K.; Vijayan, Smitha; Jisha, M. S.

doi:10.1016/j.ijbiomac.2018.03.130

Cited by 141 publications

(61 citation statements)

References 26 publications

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“…In addition, the superoxide scavenging activities of α-and β-chitosan ( Figure 5a) were significantly lower compared with their nanoparticles (Figure 5b) except for bNP2 at 0.5-1 mg/mL. Previous report stated that the antioxidant activity of chitosan increased in its nano form [28]. This may result from the less compact structure of chitosan nanoparticles than in the chitosan raw material, as indicated by %CI (Figure 8).…”

Section: Superoxide Radical Scavenging Activities Of Chitosan and Chimentioning

confidence: 82%

“…Previous studies of chitosan polymorphs have revealed differences in crystalline structure between α-, βand γ-chitosan obtained from various sources [26,27]. The antioxidant properties of chitosan-based nanoparticles have been also widely reviewed [5,28,29]. However, the impact of the crystalline structural differences between αand β-chitosan on their nanoparticle formation via ionic gelation and their superoxide radical scavenging activities has not yet been compared.The objective of this study was thus to investigate the effects of crystalline structural differences between αand β-chitosan on their nanoparticle formation via ionic gelation and their superoxide radical scavenging activities.…”

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confidence: 99%

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Impact of Crystalline Structural Differences Between α- and β-Chitosan on Their Nanoparticle Formation Via Ionic Gelation and Superoxide Radical Scavenging Activities

2019

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α- and β-Chitosan nanoparticles were obtained from shrimp shell and squid pen chitosan with different set of deacetylation degree (%DD) and molecular weight (MW) combinations. After nanoparticle formation via ionic gelation with sodium tripolyphosphate (TPP), the % crystallinity index (%CI) of the α- and β-chitosan nanoparticles were reduced to approximately 33% and 43% of the initial %CI of the corresponding α- and β-chitosan raw samples, respectively. Both forms of chitosan and chitosan nanoparticles scavenged superoxide radicals in a dose-dependent manner. The %CI of α- and β-chitosan and chitosan nanoparticles was significantly negatively correlated with superoxide radical scavenging abilities over the range of concentration (0.5, 1, 2 and 3 mg/mL) studied. High %DD, and low MW β-chitosan exhibited the highest superoxide radical scavenging activity (p < 0.05). α- and β-Chitosan nanoparticles prepared from high %DD and low MW chitosan demonstrated the highest abilities to scavenge superoxide radicals at 2.0–3.0 mg/mL (p < 0.05), whereas α-chitosan nanoparticles, with the lowest %CI, and smallest particle size (p < 0.05), prepared from medium %DD, and medium MW chitosan showed the highest abilities to scavenge superoxide radicals at 0.5–1.0 mg/mL (p < 0.05). It could be concluded that α- and β-chitosan nanoparticles had superior superoxide radical scavenging abilities than raw chitosan samples.

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Section: Superoxide Radical Scavenging Activities Of Chitosan and Chimentioning

confidence: 82%

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confidence: 99%

Impact of Crystalline Structural Differences Between α- and β-Chitosan on Their Nanoparticle Formation Via Ionic Gelation and Superoxide Radical Scavenging Activities

2019

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“…For fruits and vegetables, it is advisable to use edible films or coatings to improve the selective permeability of the gas or water, otherwise, the respiratory rate will be enhanced to accelerate the aging process and anaerobic fermentation processes will occur, leading to spoilage [1]. Among others, some biological macromolecules, including polysaccharides, e.g., starches, celluloses, and chitosan, have good coat-forming properties [6][7][8][9]. Among them, chitosan is a natural antimicrobial agent with the advantages of nontoxicity, biodegradability, and wide-spectrum utilization, which is suitable for fruit preservation [10], however, chitosan still has poor mechanical strength [11].…”

Section: Introductionmentioning

confidence: 99%

Effect of Nano-SiOx/Chitosan Complex Coating on the Physicochemical Characteristics and Preservation Performance of Green Tomato

et al. 2019

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A novel nano-silicon oxides (SiOx)/chitosan complex film was prepared using ultrasonic assistant in the process of dissolving chitosan and silicon oxides (SiOx), and characterized by transmission electron microscopy. Its effect on quality preservation of tomatoes (Solanum lycopersicum L. cv. Zheza 205) was investigated under ambient temperature. The results revealed that the nano-SiOx/chitosan complex (NSCC) film retarded weight loss and softness, delayed the titratable acids and total soluble solids loss, and thus markedly extended shelf life of green tomatoes. The antimicrobial activity of tomatoes coated with NSCC film was also recorded higher compared to chitosan (Ch) films and control. In addition, the NSCC film-coated tomatoes prevent the increase of malondialdehyde content and total polyphenol content. Moreover, the peroxidase activity, phenylalanine ammonia-lyase activity, and polyphenoloxidase activity of tomatoes coated with NSCC film were found lower than that in other treatments. These data indicated that the beneficial effects of nano-SiOx/chitosan complex coating on postharvest quality were possibly associated with the lower rate of O 2 /CO 2 transmission coefficient, limiting food-borne pathogenic bacterial growth, higher antioxidant activities, and also higher reactive oxygen species (ROS) scavenging and anti-browning activities of related enzymes in the tomatoes. Further, the results of the study could be used to successfully develop a novel nano-SiOx/chitosan complex film for improving the postharvested quality of tomatoes and thus effectively utilized by the food packaging industry.

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“…18 Previous studies have demonstrated the applications of CS in the field of medicine and pharmaceutical research due to their mucoadhesive properties as well as their antibacterial, anticancer and permeation-enhancing ability for targeted drug and protein delivery. 17,19 Notably, a composite of CS with other polymeric material has proven useful in regenerative medicine. 20 However, one drawback in the applicability of CS is the poor solubility at neutral and alkaline conditions, which decreases drug delivery efficiency at pH values above 6.…”

Section: Introductionmentioning

confidence: 99%

<p>Chitosan And N, N, N-Trimethyl Chitosan Nanoparticle Encapsulation Of <em>Ocimum Gratissimum</em> Essential Oil: Optimised Synthesis, In Vitro Release And Bioactivity</p>

Onyebuchi¹,

Kavaz²

2019

IJN

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Background: The encapsulation of plant essential oils (EOs) with polymeric materials (e.g. chitosan (CS) and N, N, N-trimethyl chitosan (TMC)) and the further reduction of the polymers into their nano sizes are gaining research interest in nanotechnology due to potential applications in medical drug delivery systems as well as the food and pharmaceutical industry. The present study reports a novel approach for the synthesis of Ocimum gratissimum essential oil (OGEO)-loaded CS and TMC nanoparticles with distinct bioactive and physiochemical properties. Methods: The OGEO-loaded CS and TMC nanoparticles were characterised using various microscopic and spectroscopic techniques. The bioactive compounds in Ocimum gratissimum methanolic extract (OG-MeOH) and EOs was evinced with gas chromatography-mass spectrometry (GC-MS). Total phenolic content (TPC) of OGEO and OG-MeOH was determined using the Folin-Ciocalteu method. The in vitro drug release kinetic pattern was ascertained by membrane dialysis, while antioxidant activity was determined by the 2,2-diphenyl-1picrylhydrozyl (DPPH) free radical scavenging method. The disc diffusion method was used for antibacterial activity evaluation, while MTT and a trypan blue dye exclusion assay were used to assess cytotoxic activity on MDA-MB-231 breast cancer cells. Results: GC-MS analysis revealed components that have not been previously reported for Ocimum gratissimum. The maximum OGEO cumulative drug release percentage in vitro was observed at pH 3 for both OGEO-loaded chitosan nanoparticles (OGEO-CSNPs) and OGEOloaded N, N, N-trimethyl chitosan nanoparticles (OGEO-TMCNPs). The antioxidant activity of OGEO-CSNPs and OGEO-TMCNPs never reached a steady state after 75 h. OGEO-TMCNPs exhibited antibacterial activity at a lower concentration for both Gram-negative and Gram-positive food pathogens. In vitro cytotoxicity revealed the increased toxicity of OGEO-TMCNPs on MDA-MB-231 breast cancer cell lines. Conclusion: OGEO-loaded CS and TMC nanoparticles were synthesised using a novel material optimisation approach. The synthesised nanoparticles have shown a promising application in the pharmaceutical and food industries.

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Antifungal, antioxidant and cytotoxic activities of chitosan nanoparticles and its use as an edible coating on vegetables

Cited by 141 publications

References 26 publications

Impact of Crystalline Structural Differences Between α- and β-Chitosan on Their Nanoparticle Formation Via Ionic Gelation and Superoxide Radical Scavenging Activities

Impact of Crystalline Structural Differences Between α- and β-Chitosan on Their Nanoparticle Formation Via Ionic Gelation and Superoxide Radical Scavenging Activities

Effect of Nano-SiOx/Chitosan Complex Coating on the Physicochemical Characteristics and Preservation Performance of Green Tomato

<p>Chitosan And N, N, N-Trimethyl Chitosan Nanoparticle Encapsulation Of <em>Ocimum Gratissimum</em> Essential Oil: Optimised Synthesis, In Vitro Release And Bioactivity</p>

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