Antifungal Drug Resistance in Aspergillus

Moore, Caroline B.; Sayers, N M; Mosquera, Juan Alejandro Neira; Slaven, J.; Denning, David W.

doi:10.1053/jinf.2000.0747

Cited by 149 publications

(110 citation statements)

References 105 publications

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“…This polyene antifungal agent binds to ergosterol to form pores that cause leakage of hyphal content and eventually death of the fungus (Moore et al, 2000). An16g05910 and An16g05920 are adjacent genes, both downregulated in our experiment.…”

Section: Upregulation Of An12g09940 and An03g00580mentioning

confidence: 60%

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

et al. 2011

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Interactions between bacteria and fungi cover a wide range of incentives, mechanisms and outcomes. The genus Collimonas consists of soil bacteria that are known for their antifungal activity and ability to grow at the expense of living fungi. In non-contact confrontation assays with the fungus Aspergillus niger, Collimonas fungivorans showed accumulation of biomass concomitant with inhibition of hyphal spread. Through microarray analysis of bacterial and fungal mRNA from the confrontation arena, we gained new insights into the mechanisms underlying the fungistatic effect and mycophagous phenotype of collimonads. Collimonas responded to the fungus by activating genes for the utilization of fungal-derived compounds and for production of a putative antifungal compound. In A. niger, differentially expressed genes included those involved in lipid and cell wall metabolism and cell defense, which correlated well with the hyphal deformations that were observed microscopically. Transcriptional profiles revealed distress in both partners: downregulation of ribosomal proteins and upregulation of mobile genetic elements in the bacteria and expression of endoplasmic reticulum stress and conidia-related genes in the fungus. Both partners experienced nitrogen shortage in each other's presence. Overall, our results indicate that the Collimonas/ Aspergillus interaction is a complex interplay between trophism, antibiosis and competition for nutrients.

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Section: Upregulation Of An12g09940 and An03g00580mentioning

confidence: 60%

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

et al. 2011

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“…The mechanisms of resistance to these antifungal agents by yeast isolates are purely chromosomal as Candida species lack plasmid or other natural mechanisms capable of transferring genetic materials between strains (Odds et al, 2003). Knowledge of mechanisms of antifungal resistance has been valuable in identifying resistant isolates and using them to validate in vitro measurement systems (VandenBossche et al, 1998;White et al, 1998;Ghannoum et al, 1999;Moore et al, 2000). This paper aimed to determine the antifungal resistance of Candida species among female patients clinically diagnosed with genitourinary tract infections with special emphasis on the wellstudied National Committee for Clinical Laboratory Standards (NCCLS, 2002) methodologies.…”

Section: Introductionmentioning

confidence: 99%

Antifungal Resistance Among <i>Candida species</i> From Patients with Genitourinary Tract Infection at Muhammad Abdullahi Wase Specialist Hospital, Kano - Nigeria

Taura¹,

Maje²,

Koki³

et al. 2013

Nig J Bas App Sci

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ABSTRACT:The increasing incidence of Candidiasis affecting the genitourinary tracts as well as the introduction of new antifungal drugs has recently highlighted the need for performing fungal susceptibility tests. To determine the antifungal resistance among Candida species from the genitourinary tracts, 689 Urine and High vaginal swab (HVS) samples were collected from female patients clinically diagnosed with genitourinary tract infection between September 2011 to January 2012. The samples were inoculated onto Sabouraud dextrose agar (SDA). Isolates from SDA were placed on Corn Med agar (CMA) to ensure detection of mixed cultures. Germ tube tests were performed for identification of the isolates. Susceptibility tests were carried on the isolates using broth dilution method. The occurrence rate of Candida species were as follows: Candida albicans 124 (48.4%), Candida glabrata 89 (34.8%), Candida krusei 23 (9.0%) and Candida Tropicalis 20 (7.8%). The rate of occurrence of Candida species in high vaginal swab 76 (61.3%) was significantly higher than that of urine 48 (38.7%). Distribution of Candida species among different age groups show that, the highest incidence was in age brackets 20 -30 158 (61.7%), while that of 41-50 and above 8 (3.1%) had the least. High rate of susceptibility was observed for each isolate against Fluconazole 23 (65.7%) and Ketoconazole 22 (62.9%). The resistance rate was 12 (34.3%) for Fluconazole and Ketoconazole 13 (37.1%). These results incriminate C. albicansas the most common Candida species causing genitourinary tract infection in women. This surveillance study has established Fluconazole and Ketoconazole as very effective antifungal agents for the treatment of genitourinary tract infections caused by Candida species.

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“…Currently, the most commonly used drugs for the treatment of aspergillosis are amphotericin B, which binds membrane sterol and creates transmembrane channels leading to an increase in membrane permeability to monovalent cations (7), and itraconazole, which competes for oxygen at the catalytic heme site of cytochrome P450/ lanosterol 14a-demethylase, leading to an inhibition of ergosterol biosynthesis and the accumulation of 14a-methylated sterols in the fungal plasma membrane (8). However, these two drugs have major disadvantages, including the nephrotoxicity of amphotericin B and the lack of itraconazole preparations for intravenous application, limiting its use in patients with impaired bowel absorption.…”

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confidence: 99%

Analysis of glycosylinositol phosphorylceramides expressed by the opportunistic mycopathogen Aspergillus fumigatus

Toledo

Levery

Bennion

et al. 2007

Journal of Lipid Research

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Acidic glycosphingolipid components were extracted from the opportunistic mycopathogen Aspergillus fumigatus and identified as inositol phosphorylceramide and glycosylinositol phosphorylceramides (GIPCs). Using nuclear magnetic resonance sppectroscopy, mass spectrometry, and other techniques, the structures of six major components were elucidated as Ins-P-Cer (, and Manp(a1Y3)Manp(a1Y6)GlcpN(a1Y2)Ins-PCer (Af-3c) (where Ins 5 myo-inositol and P 5 phosphodiester). A minor A. fumigatus GIPC was also identified as the N-acetylated version of Af-3c (Af-3c*), which suggests that formation of the GlcNa1Y2Ins linkage may proceed by a two-step process, similar to the GlcNa1Y6Ins linkage in glycosylphosphatidylinositol (GPI) anchors (transfer of GlcNAc, followed by enzymatic de-N-acetylation). The glycosylinositol of Af-3b, which bears a distinctive branching Galf (b1Y6) residue, is identical to that of a GIPC isolated previously from the dimorphic mycopathogen Paracoccidioides brasiliensis (designated Pb-3), but components Af-3a and Af-4 have novel structures. Overlay immunostaining of A. fumigatus GIPCs separated on thinlayer chromatograms was used to assess their reactivity against sera from a patient with aspergillosis and against a murine monoclonal antibody (MEST-1) shown previously to react with the Galf (b1Y6) residue in Pb-3. These results are discussed in relation to pathogenicity and potential approaches to the immunodiagnosis of A. fumigatus.-Toledo, M. S., S. B. Levery, B. Bennion, L. L.

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Antifungal Drug Resistance in Aspergillus

Cited by 149 publications

References 105 publications

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

Antifungal Resistance Among <i>Candida species</i> From Patients with Genitourinary Tract Infection at Muhammad Abdullahi Wase Specialist Hospital, Kano - Nigeria

Analysis of glycosylinositol phosphorylceramides expressed by the opportunistic mycopathogen Aspergillus fumigatus

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