Antifungal effect of the bark and root extracts of Punica granatum on oral Candida strains isolated from liver recipients

Lavaee, Fatemeh; Motaghi, Darya; Jassbi, Amir Reza; Jafarian, Hadis; Ghasemi, Fatemeh; Badiee, Parisa

doi:10.18502/cmm.4.4.382

Cited by 12 publications

(9 citation statements)

References 22 publications

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“…It is used in folk medicine to treat various diseases such as ulcers, fever, diarrhea, and microbial infections ( Endo et al, 2010 ). In addition, different parts of P. granatum , such as the leaves, flowers, and seeds, have antimicrobial, antioxidant, and antifungal properties ( Lavaee et al, 2018 ). The antifungal activity of Punica granatum extract has already been reported against some species of Candida ( Anibal et al, 2013 ; Mansourian et al, 2014 ; Akroum, 2017 ; Paul et al, 2018 ) and against dermatophyte fungi ( Foss et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

A Study of the Disruptive Effect of the Acetate Fraction of Punica granatum Extract on Cryptococcus Biofilms

et al. 2021

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Cryptococcosis, caused by yeasts of the genus Cryptococcus, is an infectious disease with a worldwide distribution. Cryptococcus neoformans and Cryptococcus gattii are the species that commonly cause this disease in humans; however, infections caused by Cryptococcus laurentii, especially in immunocompromised patients, are increasingly being reported. Owing to the increase in the resistance of fungi to antifungals, and a lack of treatment options, it is important to seek new therapeutic alternatives such as natural products. Among these are plant species such as Punica granatum, which is used in folk medicine to treat various diseases. This study aimed to evaluate the activity of the acetate fraction of P. granatum leaf extract against environmental and clinical isolates of Cryptococcus. Three environmental isolates of C. laurentii, PMN, PMA, and PJL II, isolated from soils of different municipalities in the state of Maranhão, a clinical isolate, C. gattii, from a patient with neurocryptococcosis, and a standard strain of C. gattii (ATCC 32068) were used. The minimum and fractional inhibitory concentrations (MIC and FIC, respectively) and time-kill curve of the extract and fluconazole were determined to assess the susceptibility profile of the fungal isolates. Larvae of Tenebrio molitor were infected with Cryptococcus strains, and the effects of acetate fraction of P. granatum extract and fluconazole on the survival and fungal burden were determined. The extract activity was tested against pre-formed biofilms. The acetate fraction of P. granatum extract showed promising antifungal activity against all the species of Cryptococcus evaluated in this study, with an MIC value lower than that of fluconazole. The indices obtained in the FIC test indicated that the antimicrobial effect of the combination of the extract and antifungal was indifferent for 80% of the isolates. The P. granatum acetate fraction reduced the pre-formed biofilm of some isolates, showing better activity than fluconazole, which is consistent with results from fluorescence microscopy. This is the first study on the use of P. granatum and its ability to inhibit Cryptococcus biofilms; therefore, further studies and tests are needed to investigate the components and mechanism of action of P. granatum against cryptococcosis agents.

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Section: Introductionmentioning

confidence: 99%

A Study of the Disruptive Effect of the Acetate Fraction of Punica granatum Extract on Cryptococcus Biofilms

et al. 2021

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“…Lavaee et al . compared the efficacy of the methanolic and ethanolic extracts of the bark and roots of Punica in the isolation of Candida species and found that the methanolic extracts were more effective [ 38 ]. Accordingly, in the current study, ethanolic extracts were used.…”

Section: Discussionmentioning

confidence: 99%

Comparison of antifungal efficacy of ethanolic extracts of Woodfordia fruticosa leaf and Punica granatum peel in uncontrolled diabetic patients wearing removable dentures: A randomized controlled clinical trial

Sujanamulk

et al. 2020

CMM

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Background and Purpose: The search for the development of a suitable novel antimicrobial agent for fungal diseases continues to be a key problem in the current clinical field. The present investigation was aimed to determine the antifungal effect of the ethanolic crude extracts of Woodfordia fruticosa leaf (Wfl) and Punica granatum peel (Pgp) in uncontrolled diabetic patients wearing removable dentures. Materials and Methods: The ethanolic extracts of both plants were prepared using the soxhlet extraction method, and the obtained metabolites were confirmed by thin-layer chromatography. After the preparation of the mouthwash, a total of 100 subjects were randomly divided into two groups. Each subject was given physiological saline at the baseline. Group I was provided with P. granatum mouthwash, while Group II was given W. fruticosa mouthwash. Following the administration of the mouthwash, the patients were requested to rinse the mouthwash using the oral rinse technique twice daily 5 ml/rinse for 30 sec. Subsequently, colony-forming units (CFU) were evaluated in the participants. Post-therapeutic samples were collected 1 h and 1 week after the mouthwash use. Results: The mean reduction of CFU was calculated at the baseline, as well as 1 h and 1 week after using mouthwash. The results indicated a drastic reduction in CFU 1 h and 1 week after the application of Wfl mouthwash. Conclusion: The obtained data revealed that Wfl had potential anticandidal activity against Candida yeast cells, probably owing to its bioactive compounds like glycosides. Therefore, this agent can be used effectively as a natural remedy for the treatment of oral candidiasis. However, the exact mechanism of action of this plant needs to be elucidated.

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“…The treated Petri dishes were placed at 4°C for 1-2 hours and then incubated for 48 hr at 37°C. Nystatin 0.2 mg/ml was used as the reference drug [ 27 ]. At the end of the period, the inhibition zones formed on the media were measured with a transparent ruler in millimeters.…”

Section: Methodsmentioning

confidence: 99%

Phytochemical Profile and Antimicrobial Activities of Edible Mushroom Termitomyces striatus

Sitati

Ogila

Waihenya

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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The mushroom Termitomyces striatus is an edible mushroom that grows wildly and belongs to the family Lyophyllaceae. Studies in the last few decades have demonstrated that mushrooms and their active components have beneficial effects on a variety of biological systems. Some mushrooms do exhibit antibacterial properties. Qualitative phytochemical profile was done on the mushroom Termitomyces striatus to establish the presence of compounds responsible for important biological activities. This study also investigated the effect of Termitomyces striatus extracts on certain bacterial strains that included Escherichia coli and Pseudomonas aeruginosa representing the Gram-negative bacteria and Bacillus subtilis and Staphylococcus aureus representing Gram-positive bacteria. The fungi were represented by Candida albicans and Saccharomyces cerevisiae. The mushroom was collected in western Kenya, air-dried, and crushed into powder, followed by extraction using water, methanol, and dichloromethane (DCM) solvents. Antibacterial and antifungal activities were evaluated using the disc-diffusion method. Qualitative phytochemical screening of the aqueous extract revealed the presence of alkaloids, flavonoids, steroids, sterols, saponins, phenols, carbohydrates, and proteins. The three extracts exhibited antibacterial against tested bacterial strains. The DCM extract revealed higher effects among the bacterial strains tested. The three extracts showed antifungal effects against C. albicans. However, both methanol and aqueous extracts did not inhibit growth of S. cerevisiae. In conclusion, T. striatus extracts are a promising source of novel antimicrobial and antifungal agents.

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Antifungal effect of the bark and root extracts of Punica granatum on oral Candida strains isolated from liver recipients

Cited by 12 publications

References 22 publications

A Study of the Disruptive Effect of the Acetate Fraction of Punica granatum Extract on Cryptococcus Biofilms

A Study of the Disruptive Effect of the Acetate Fraction of Punica granatum Extract on Cryptococcus Biofilms

Comparison of antifungal efficacy of ethanolic extracts of Woodfordia fruticosa leaf and Punica granatum peel in uncontrolled diabetic patients wearing removable dentures: A randomized controlled clinical trial

Phytochemical Profile and Antimicrobial Activities of Edible Mushroom Termitomyces striatus

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