Antifungal effect of volatile organic compounds produced by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 on oxidative stress and mitochondrial dysfunction of Ceratocystis fimbriata

Zhang, Yu; Li, Tengjie; Xu, Mingyu; Guo, Jianheng; Zhang, Chunmei; Feng, Zhaozhong; Peng, Xue; Li, Zongyun; Xing, Ke; Qin, Sheng

doi:10.1016/j.pestbp.2021.104777

Cited by 47 publications

(24 citation statements)

References 46 publications

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“…The LD 50 in mice is 230 mg/kg body weight [16]. Ipomeamarone can cause liver and lung poisoning in humans and animals [17,18], resulting in respiratory distress, pulmonary oedema, congestion, and even livestock death [19].…”

Section: Introductionmentioning

confidence: 99%

Early Discrimination and Prediction of C. fimbriata-Infected Sweetpotatoes during the Asymptomatic Period Using Electronic Nose

Pang

Zhang

et al. 2022

Foods

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Sweetpotato is prone to disease caused by C. fimbriata without obvious lesions on the surface in the early period of infection. Therefore, it is necessary to explore the possibility of developing an efficient early disease detection method for sweetpotatoes that can be used before symptoms are observed. In this study, sweetpotatoes were inoculated with C. fimbriata and stored for different lengths of time. The total colony count was detected every 8 h; HS-SPME/GC–MS and E-nose were used simultaneously to detect volatile compounds. The results indicated that the growth of C. fimbriata entered the exponential phase at 48 h, resulting in significant differences in concentrations of volatile compounds in infected sweetpotatoes at different times, especially toxic ipomeamarone in ketones. The contents of volatile compounds were related to the responses of the sensors. E-nose was combined with multiple chemometrics methods to discriminate and predict infected sweetpotatoes at 0 h, 48 h, 64 h, and 72 h. Among the methods used, linear discriminant analysis (LDA) had the best discriminant effect, with sensitivity, specificity, precision, and accuracy scores of 100%. E-nose combined with K-nearest neighbours (KNN) achieved the best predictions for ipomeamarone contents and total colony counts. This study illustrates that E-nose is a feasible and promising technology for the early detection of C. fimbriata infection in sweetpotatoes during the asymptomatic period.

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Section: Introductionmentioning

confidence: 99%

Early Discrimination and Prediction of C. fimbriata-Infected Sweetpotatoes during the Asymptomatic Period Using Electronic Nose

Pang

Zhang

et al. 2022

Foods

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“…Considering that the intracellular ATP content directly affects the normal energy metabolism of pathogenic fungi, is a general energy source for all living cells, and provides direct energy for various life activities of organisms [ 25 ], the effects of isoxanthohumol on ATP content and ATPase activity were also studied. As shown in Figure 9 C, the ATP content in B. cinerea was 0.9737 μmol/mL in the blank control; when treated with 5.0 and 10.0 μg/mL of isoxanthohumol, the ATP content reduced to 0.7859 μmol/mL and 0.3590 μmol/mL, respectively, and ATPase activity was decreased by 59.74% and 77.29%, respectively, at the same concentrations.…”

Section: Resultsmentioning

confidence: 99%

The Antifungal Mechanism of Isoxanthohumol from Humulus lupulus Linn.

Yan

et al. 2021

IJMS

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Humulus lupulus Linn. is a traditional medicinal and edible plant with several biological properties. The aims of this work were: (1) to evaluate the in vitro antifungal activity of H. lupulus ethanolic extract; (2) to study the in vitro and in vivo antifungal activity of isoxanthohumol, an isoprene flavonoid from H. lupulus, against Botrytis cinerea; and (3) to explore the antifungal mechanism of isoxanthohumol on B. cinerea. The present data revealed that the ethanolic extract of H. lupulus exhibited moderate antifungal activity against the five tested phytopathogenic fungi in vitro, and isoxanthohumol showed highly significant antifungal activity against B. cinerea, with an EC50 value of 4.32 µg/mL. Meanwhile, it exhibited moderate to excellent protective and curative efficacies in vivo. The results of morphologic observation, RNA-seq, and physiological indicators revealed that the antifungal mechanism of isoxanthohumol is mainly related to metabolism; it affected the carbohydrate metabolic process, destroyed the tricarboxylic acid (TCA) cycle, and hindered the generation of ATP by inhibiting respiration. Further studies indicated that isoxanthohumol caused membrane lipid peroxidation, thus accelerating the death of B. cinerea. This study demonstrates that isoxanthohumol can be used as a potential botanical fungicide for the management of phytopathogenic fungi.

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“…The damages are similar to tea tree oil (Li et al ., 2017), volatile organic compounds produced by Pseudomonas chlororaphis subsp. Aureofaciens SPS‐41 (Zhang et al ., 2021), and citral (Zheng et al ., 2015) studied previously.…”

Section: Discussionmentioning

confidence: 99%

Antifungal effect of o‐vanillin on mitochondria of Aspergillus flavus: ultrastructure and TCA cycle are destroyed

Zhao

Zhu

et al. 2022

Int J of Food Sci Tech

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Aspergillus flavus (A. flavus) is a conditioned pathogen that severely endangers food safety. This study is aimed to determine the mode of action of o‐vanillin against A. flavus mitochondria. First, o‐vanillin hindered respiration. Furthermore, the mitochondrial ultrastructures were damaged with scanning electron microscopy (SEM) and transmission electron microscopy (TEM) characterisation. The activities of the tricarboxylic acid (TCA) cycle‐related enzymes were regulated significantly (P < 0.05), and the most significant reduction and promotion was citrate synthase (CS) and malic dehydrogenase (MDH), respectively, at 100 μg mL−1 of o‐vanillin. Consistent with the above phenotypic results, real‐time quantitative reverse transcription polymerase chain reaction (PCR) (QRT‐PCR) results showed that the relative expressions of genes encoding the TCA‐related enzymes were regulated remarkably (P < 0.05), especially for CIT1 gene (downregulated) and MDH gene (upregulated). This study demonstrated the damage of o‐vanillin on mitochondria and shed light on the antifungal mechanism of o‐vanillin against A. flavus.

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Antifungal effect of volatile organic compounds produced by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 on oxidative stress and mitochondrial dysfunction of Ceratocystis fimbriata

Cited by 47 publications

References 46 publications

Early Discrimination and Prediction of C. fimbriata-Infected Sweetpotatoes during the Asymptomatic Period Using Electronic Nose

Early Discrimination and Prediction of C. fimbriata-Infected Sweetpotatoes during the Asymptomatic Period Using Electronic Nose

The Antifungal Mechanism of Isoxanthohumol from Humulus lupulus Linn.

Antifungal effect of o‐vanillin on mitochondria of Aspergillus flavus: ultrastructure and TCA cycle are destroyed

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