Antifungal Effects of Volatiles Produced by Bacillus subtilis Against Alternaria solani in Potato

Dai, Zhongliang; Yu, Shuiqing; Yang, Yiqing; Zhang, Jinglin; Zhao, Dongmei; Pan, Yang; Fan, Shasha; Yang, Zhihui; Zhu, Jun‐Jie

doi:10.3389/fmicb.2020.01196

Cited by 87 publications

(69 citation statements)

References 44 publications

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“…In contrast, a volatile organic compound (VOC) assay revealed that none of the isolated exhibited inhibitory activity against any of the tested fungi. These results conflict with previous studies in which VOC inhibitory activity by Bacillus strains against Aspergillus , Fusarium , and Alternaria species has been reported [ 40 , 41 , 42 ].…”

Section: Resultscontrasting

confidence: 99%

Analysis of Stored Wheat Grain-Associated Microbiota Reveals Biocontrol Activity among Microorganisms against Mycotoxigenic Fungi

Solanki

Abdelfattah

Sudharsan

et al. 2021

JoF

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Wheat grains are colonized by complex microbial communities that have the potential to affect seed quality and susceptibility to disease. Some of the beneficial microbes in these communities have been shown to protect plants against pathogens through antagonism. We evaluated the role of the microbiome in seed health: in particular, against mycotoxin-producing fungi. Amplicon sequencing was used to characterize the seed microbiome and determine if epiphytes and endophytes differ in their fungal and bacterial diversity and community composition. We then isolated culturable fungal and bacterial species and evaluated their antagonistic activity against mycotoxigenic fungi. The most prevalent taxa were found to be shared between the epiphytic and endophytic microbiota of stored wheat seeds. Among the isolated bacteria, Bacillus strains exhibited strong antagonistic properties against fungal pathogens with noteworthy fungal load reduction in wheat grain samples of up to a 3.59 log10 CFU/g compared to untreated controls. We also found that a strain of the yeast, Rhodotorula glutinis, isolated from wheat grains, degrades and/or metabolizes aflatoxin B1, one of the most dangerous mycotoxins that negatively affects physiological processes in animals and humans. The mycotoxin level in grain samples was significantly reduced up to 65% in the presence of the yeast strain, compared to the untreated control. Our study demonstrates that stored wheat grains are a rich source of bacterial and yeast antagonists with strong inhibitory and biodegradation potential against mycotoxigenic fungi and the mycotoxins they produce, respectively. Utilization of these antagonistic microorganisms may help reduce fungal and mycotoxin contamination, and potentially replace traditionally used synthetic chemicals.

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Section: Resultscontrasting

confidence: 99%

Analysis of Stored Wheat Grain-Associated Microbiota Reveals Biocontrol Activity among Microorganisms against Mycotoxigenic Fungi

Solanki

Abdelfattah

Sudharsan

et al. 2021

JoF

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“…These micro-organisms include bacteria such as Bacillus sp. (Zheng et al 2013;Morita et al 2019;Zhang et al 2020), Pseudomonas sp. (Elkahoui et al 2015) and Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Antifungal effect of volatile organic compounds produced byStreptomyces salmonisPSRDC‐09 against anthracnose pathogenColletotrichum gloeosporioidesPSU‐03 in postharvest chili fruit

Boukaew

Cheirsilp

Prasertsan

et al. 2021

J of Applied Microbiology

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Aims: Application of volatile organic compounds (VOCs) from Streptomyces salmonis PSRDC-09 (VOCs PSRDC-09) grown on sterile wheat seeds against chili anthracnose pathogen was investigated in vitro and in vivo. Methods and Results: Among 10 isolates of Colletotrichum species tested, Colletotrichum gloeosporioides PSU-03 was selected as the most aggressive anthracnose pathogenic strain on chili fruit against VOCs produced by Streptomyces species. Among 11 isolates, the strain PSRDC-09 exhibited the highest antifungal activity and was identified as S. salmonis PSRDC-09. The antagonistic mechanism of the VOCs PSRDC-09 on morphological of C. gloeosporioides PSU-03, observed by scanning electron microscope (SEM), revealed the irregular distortions in the fungal hyphae. The effect of inoculum size and spore concentration of S. salmonis PSRDC-09 prepared as a wheat seed inoculum on the suppression of C. gloeosporioides PSU-03 was studied both in vitro and on chili fruit. The optimum inoculum size (45 g 0Á31 l À1 ) and spore concentration (10 7 spores per ml) of the wheat seed culture of S. salmonis PSRDC-09 exhibited the complete suppression (100% inhibition) on C. gloeosporioides PSU-03. The optimum fumigation period of the VOCs PSRDC-09 (45 g 1Á38 l À1 ) was found to be 24 h. Based on gas chromatography-mass spectrometry (GC-MS) analysis, 14 major VOCs (produced by the strain PSRDC-09) were detected and l-linalool was the main volatile component. Conclusions:The results indicated that the VOCs from S. salmonis PSRDC-09 could effectively control the chili anthracnose disease caused by C. gloeosporioides. Significance and Impact of the Study: These findings suggest that S. salmonis PSRDC-09 may have the potential to become a promising biofumigant for biocontrol of chili anthracnose disease in the postharvest system.

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“…In another study, 3 lipopeptides from B. subtilis were described to be effective against Venturia inaequalis, fungi causing apple scab [30]. Zhang et al also proved the efficacy of volatile oil compounds produced by Bacillus strain ZD01, against A. solani, a fungal pathogen causing diseases of potato [31]. Moreover, B. subtilis subsp.…”

Section: Discussionmentioning

confidence: 99%

Optimization of antifungal activity by Bacillus subtilis isolate CCASU 2021-4 using Response Surface Methodology

El-Housseiny

Shams

Ghobashi

et al. 2021

Archives of Pharmaceutical Sciences Ain Shams University

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Fungal infections represent an enormous load on the public especially with the development of resistance to the most currently used antifungal drugs in practice. In the present work, a bacterial isolate coded A3 was recovered from soil and was shown to express antagonistic activity against Candida (C.) albicans ATCC 10231. This isolate was identified as Bacillus (B.) subtilis subsp. spizizenii isolate CCASU 2021-4 using 16S ribosomal RNA sequencing. D-optimal design from response surface methodology (RSM) was used to optimize the environmental variables affecting the antifungal activity of the respective isolate. The optimum conditions were a temperature of 30 °C, a pH of 8, and an inoculum size of 5 x 10 7 cfu/mL, resulting in an enhancement in the antifungal activity by 1.2 fold. This is the first report, to the best of our knowledge, on an antifungal activity from B. subtilis subsp. spizizenii culture broth against human fungal pathogens along with its optimization through RSM.

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Antifungal Effects of Volatiles Produced by Bacillus subtilis Against Alternaria solani in Potato

Cited by 87 publications

References 44 publications

Analysis of Stored Wheat Grain-Associated Microbiota Reveals Biocontrol Activity among Microorganisms against Mycotoxigenic Fungi

Analysis of Stored Wheat Grain-Associated Microbiota Reveals Biocontrol Activity among Microorganisms against Mycotoxigenic Fungi

Antifungal effect of volatile organic compounds produced byStreptomyces salmonisPSRDC‐09 against anthracnose pathogenColletotrichum gloeosporioidesPSU‐03 in postharvest chili fruit

Optimization of antifungal activity by Bacillus subtilis isolate CCASU 2021-4 using Response Surface Methodology

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