Antifungal Mechanism of a Novel Antifungal Protein from Pumpkin Rinds against Various Fungal Pathogens

Gopal

et al. 2013

IJMS

Self Cite

In an earlier study, we isolated potamin-1 (PT-1), a 5.6-kDa trypsin-chymotrypsin protease inhibitor, from the tubers of a potato strain (Solanum tuberosum L cv. Gogu Valley). We established that PT-1 strongly inhibits pathogenic microbial strains, but not human bacterial strains, and that its sequence shows 62% homology with a serine protease inhibitor. In the present study, we isolated an antifungal and antibacterial peptide with no cytotoxicity from tubers of the same potato strain. The peptide (peptide-G2, PG-2) was isolated using salt-extraction, ultrafiltration and reverse-phase high performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) showed the protein to have a molecular mass of 3228.5 Da, while automated Edman degradation showed the N-terminal sequence of PG-2 to be LVKDNPLDISPKQVQALCTDLVIRCMCCC-. PG-2 exhibited antimicrobial activity against Candida albicans, a human pathogenic yeast strain, and Clavibacter michiganensis subsp. michiganensis, a plant late blight strain. PG-2 also showed antibacterial activity against Staphylococcus aureus, but did not lyse human red blood cells and was thermostable. Overall, these results suggest PG-2 may be a good candidate to serve as a natural antimicrobial agent, agricultural pesticide and/or food additive.

Section: Methodsmentioning

confidence: 99%

PG-2, a Potent AMP against Pathogenic Microbial Strains, from Potato (Solanum tuberosum L cv. Gogu Valley) Tubers Not Cytotoxic against Human Cells

Gopal

et al. 2013

IJMS

Self Cite

“…Black pumpkin seeds have another component, named cucurmoschin and identified as an antifungal peptide abundant in glycine, arginine, and glutamate residues. Additionally, Park and et al [133] found two novel antifungal proteins (Pr-1 and Pr-2) from pumpkin rinds that showed strong in vitro antifungal activity against Botrytis cinerea, Colletotrichum coccodes, Fusarium solani, F. oxysporum, and Trichoderma harzianum at 10-20 µM.…”

Section: Cucurbita Plants For Industrial Purposes: Key Role As a Foodmentioning

confidence: 99%

Cucurbita Plants: From Farm to Industry

et al. 2019

The Cucurbita genus, a member of Cucurbitaceae family, also known as cucurbits, is native to the Americas. Genus members, like Cucurbita pepo and Cucurbita maxima, have been used for centuries in folk medicine for treating gastrointestinal diseases and intestinal parasites. These pharmacological effects are mainly attributed to their phytochemical composition. Indeed, Cucurbita species are a natural source of carotenoids, tocopherols, phenols, terpenoids, saponins, sterols, fatty acids, functional carbohydrates, and polysaccharides, that beyond exerting remarkable biological effects, have also been increasingly exploited for biotechnological applications. In this article, we specifically cover the habitat, cultivation, phytochemical composition, and food preservative abilities of Cucurbita plants.

“…Lactobacilli strains can not only act against the growth of fungi, but can also act against the production of mycotoxins (Hudacek et al 2007). A series of studies on LAB indicated that some of the antifungal activity is caused by organic acids, low pH, proteinaceous compounds and other end products (Park et al 2009;Schnurer and Magnusson 2005;Hassan and Bullerman 2008). The antifungal protein produced by Enterococcus faecalis CHD 28.3 was partially purified by Roy et al (2009), the molecular mass of the antifungal protein from the high resolution gel filtration was estimated to be around 11 kDa.…”

Section: Discussionmentioning

confidence: 99%

Activity against plant pathogenic fungi of Lactobacillus plantarum IMAU10014 isolated from Xinjiang koumiss in China

et al. 2011

Thirty-five of the 77 colonies of lactic acid bacteria isolated from koumiss showed antifungal activity against Botrytis cinerea, when tested by the Poison Food Technique. Of these, the most promising isolate having a broad spectrum of antifungal activity, including against Botrytis cinerea, Alternaria solani, Phytophthora drechsleri Tucker, Fusarium oxysporum and Glomerella cingulata, was identified as Lactobacillus plantarum IMAU10014. The effect of pH, temperature and protease on the antifungal activity of L. plantarum IMAU10014 was determined. The activity substance was heat stable. The maximum antifungal activity was observed at pH 4.0. When the supernatant was adjusted to pH 8.0, the activity was lost irreversibly. However, after enzymatic treatment of supernatant with trypsin, neutral protease and proteinase K, loss of part of the antifungal activity was observed which indicated there are other substances in addition to the proteinaceous substance with the antifungal activity.