Antifungal Metabolites of Streptomyces chrestomyceticus STR-2 Inhibits Magnaporthe oryzae, the Incitant of Rice Blast

Rahila, R; Harish, S.; Kalpana, K.; Anand, Gautam; Arulsamy, M; Kalaivanan, R

doi:10.1007/s00284-023-03205-3

Cited by 6 publications

(2 citation statements)

References 47 publications

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“…Many studies revealed the impact of different actinobacterial strains on A. flavus growth and more generally on several phytopathogenic fungi. Indeed, Rahila et al demonstrated that Streptomyces chrestomyceticus STR-2 was able to reduce Magnaporthe oryzae growth with a growth inhibition of 50% [ 58 ]. Moreover, by using the same method, Shahid et al (2021) revealed that five actinobacterial strains ( Amycolatopsis pretoriensis MSCA21, Saccharopolyspora shandongensis MSCA89, Kribbella karoonensis MSCA185, S. amritsarensis V31, and S. amritsarensis V4) reduced Rhizoctonia solani , Alternaria alternata , A. flavus , Fusarium oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

Effect of Streptomyces roseolus Cell-Free Supernatants on the Fungal Development, Transcriptome, and Aflatoxin B1 Production of Aspergillus flavus

Maud

Boyer

Durrieu

et al. 2023

Toxins

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Crop contamination by aflatoxin B1 (AFB1), an Aspergillus-flavus-produced toxin, is frequently observed in tropical and subtropical regions. This phenomenon is emerging in Europe, most likely as a result of climate change. Alternative methods, such as biocontrol agents (BCAs), are currently being developed to reduce the use of chemicals in the prevention of mycotoxin contamination. Actinobacteria are known to produce many bioactive compounds, and some of them can reduce in vitro AFB1 concentration. In this context, the present study aims to analyze the effect of a cell-free supernatant (CFS) from Streptomyces roseolus culture on the development of A. flavus, as well as on its transcriptome profile using microarray assay and its impact on AFB1 concentration. Results demonstrated that in vitro, the S. roseolus CFS reduced the dry weight and conidiation of A. flavus from 77% and 43%, respectively, and was therefore associated with a reduction in AFB1 concentration reduction to levels under the limit of quantification. The transcriptomic data analysis revealed that 5198 genes were differentially expressed in response to the CFS exposure and among them 5169 were downregulated including most of the genes involved in biosynthetic gene clusters. The aflatoxins’ gene cluster was the most downregulated. Other gene clusters, such as the aspergillic acid, aspirochlorine, and ustiloxin B gene clusters, were also downregulated and associated with a variation in their concentration, confirmed by LC-HRMS.

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Section: Discussionmentioning

confidence: 99%

Effect of Streptomyces roseolus Cell-Free Supernatants on the Fungal Development, Transcriptome, and Aflatoxin B1 Production of Aspergillus flavus

Maud

Boyer

Durrieu

et al. 2023

Toxins

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“…S. graminearus can produce diverse antibiotics displaying a broad spectrum of antimicrobial activity. It has therefore been used to fight plant diseases caused by various pathogenic bacteria, fungi, and other microorganisms ( Rahila et al, 2023 ). Previous studies have shown that different Streptomyces species can inhibit mycelial growth of fungal pathogens ranging from 22 to 98%, such as S. palmae PC 12, S. vinaceusdrappus , S. philanthi RM-1-138, S. globisporus JK-1, S. griseofuscus , S. hygroscopicus , S. griseus strain SKB2.14, S. hygroscopicus strain OsiSh-2, and Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a broad-spectrum antifungal strain, Streptomyces graminearus STR-1, against Magnaporthe oryzae

Shen,

Liu,

Wang

et al. 2024

Front. Microbiol.

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Fungal diseases such as the devastating rice blast pose severe threats to crop production worldwide. Biological control of crop diseases caused by fungal pathogens is an environment-friendly approach for safeguarding crop production. But the insufficient availability of microbial agents effective against various fungal diseases has hampered the development of green production in crops. In this study, we identified a broad-spectrum antifungal bacterium, Streptomyces graminearus STR-1, showing antagonistic activity to diverse fungal pathogens including Magnaporthe oryzae, Rhizoctonia solani, Fusarium graminearum, Ustilaginoidea virens, and Bipolaris maydis. Its antifungal activity was relatively stable and less affected by temperature and pH. Evaluation of the biocontrol activity of STR-1 revealed that STR-1 prevented and controlled rice blast disease via eliciting plant immunity and suppressing fungal infection-structure development. STR-1 broth extract inhibited spore germination, likely through inhibiting protein synthesis. Combining LC–MS and chromatography analysis of the antimicrobial compounds purified from STR-1 broth extract, together with decoding STR-1 genomic sequence, we identified 4-oxo-4-[(1-phenylethyl)amino]but-2-enoic acid, 1,3,5-Trimethylpyrazole and SMA-1 as the potential main STR-1 secondary metabolites associated with its antifungal effects. This study suggests that bacterial strain STR-1 could be used for identifying highly effective and broad-spectrum secondary metabolites for containing rice blast and other crop diseases. The application of the active compounds offers a promising measure to tackle fungal disease.

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Analysis of Bioactive Compounds Produced by Bacillus mojavensis ZA1 and Their Antagonistic Effect on Colletotrichum coccodes by GC–MS

Wei,

Fan,

Yang

et al. 2023

Appl Biochem Biotechnol

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Antifungal Metabolites of Streptomyces chrestomyceticus STR-2 Inhibits Magnaporthe oryzae, the Incitant of Rice Blast

Cited by 6 publications

References 47 publications

Effect of Streptomyces roseolus Cell-Free Supernatants on the Fungal Development, Transcriptome, and Aflatoxin B1 Production of Aspergillus flavus

Effect of Streptomyces roseolus Cell-Free Supernatants on the Fungal Development, Transcriptome, and Aflatoxin B1 Production of Aspergillus flavus

Characterization of a broad-spectrum antifungal strain, Streptomyces graminearus STR-1, against Magnaporthe oryzae

Analysis of Bioactive Compounds Produced by Bacillus mojavensis ZA1 and Their Antagonistic Effect on Colletotrichum coccodes by GC–MS

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