Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247

Abstract

Aneurinibacillus: sp. YR247 was newly isolated from the deep-sea sediment inside the Calyptogena community at a depth of 1171 m in Sagami Bay. The strain exhibited antifungal activity against the filamentous fungus Aspergillus brasiliensis NBRC9455. A crude extract prepared from the YR247 cells by ethanol extraction exhibited broad antimicrobial activities. The antifungal compound is stable at 4–70 °C and pH 2.0–12.0. After treatment with proteinase K, the antifungal activity was not detected, indicating that the antifungal compound of strain YR247 is a peptidic compound. Electrospray ionization mass spectrometry of the purified antifungal compound indicated that the peptidic compound has an average molecular weight of 1167.9. The molecular weight of the antifungal compound from strain YR247 is different from those of antimicrobial peptides produced by the related Aneurinibacillus and Bacillus bacteria. The antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247 may be useful as a biocontrol agent.

Taxonomy of actinomycetes in the deep-sea Calyptogena communities and characterization of the antibacterial compound produced by Actinomadura sp. DS-MS-114

“…We have already isolated Aneurinibacillus sp. YR247 belonging to the heterotrophic Firmicutes from the Calyptogena community in Sagami Bay at a depth of 1171 m and showed that the bacterial isolate exhibits various antimicrobial activities [12]. Although we demonstrated that the Calyptogena community is useful as a bacterial isolation source, taxonomic classification of deep-sea actinomycetes in the community is uncertain and little is known about the actinomycetes producing antimicrobial compounds in the Calyptogena community.…”

Characterization and heterologous expression of an antimicrobial peptide fromBacillus amyloliquefaciensCMW1

“…Antibiotic peptides, such as amylocyclicin FZB42 from B. amyloliquefaciens FZB42 [10], plantaricyclin A from Lactobacillus plantarum NI326 [18] and antifungal peptide from Aneurinibacillus sp. YR247 [19] have been detected using mass spectral analysis. We investigated whether the recombinant amylocyclicin CMW1 is a circular peptide or a linear peptide using mature amylocyclicin CMW1 His-tag.…”

“…Using 10 mM potassium phosphate buffer (pH 7.0) and MeOH, a 50 lg/mL debris fraction was prepared. In order to detect mature amylocyclicin CMW1 His-tag, LC-MS analysis with the debris fraction was carried out [19,20]. The molecular weight of mature amylocyclicin CMW1 His-tag was estimated to be 10,041.2 because the molecular ion peaks at m/z 327.1, 348.3 and 559.9 probably correspond to the charged ions The molecular weight was estimated as 10,080.3 using the average of the values (10,109.1, 10,071.7 and 10,060.2).…”

“…The molecular weight of mature amylocyclicin CMW1 His-tag was estimated to be 10,041.2 because the molecular ion peaks at m/z 327.1, 348.3 and 559.9 probably correspond to the charged ions The molecular weight was estimated as 10,080.3 using the average of the values (10,109.1, 10,071.7 and 10,060.2). The estimated molecular weight (10,080.3) probably consists of mature amylocyclicin CMW1 His-tag (molecular weight 10,041.2) and a potassium ion (molecular weight 39.1) [19]. Therefore, recombinant mature amylocyclicin CMW1 His-tag is considered to be a linear peptide, not a circular peptide.…”

Short Communication: Antagonistic activity of bacteria isolated from coral Acropora sp of Karimunjawa Islands, Indonesia against Acroporid White Syndrome

“…In the marine environment, members of the family Bacillaceae (Bacillus, Virgibacillus, Oceanobacillus) known for the production of metabolites with antimicrobial and antifungal properties (Kurata et al, 2017). However, there was a lack of information regarding genus Virgibacillus that produce antimicrobial substances.…”