Antifungal susceptibility and virulence profile of candida isolates from abnormal vaginal discharge of women from southern India

Arumugam, G; Prabhusaran, N; Mahalingam, Pramila; Balasubramanian, Saranya; Archunan, Priya Aarthy; Archunan, Govindaraju; Rajendran, R.

doi:10.1016/j.ejogrb.2020.09.021

Cited by 2 publications

(1 citation statement)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antifungal susceptibility testing of all Candida isolates in the current study demonstrated that AMB and VRC had excellent antifungal activity against all of the species of Candida evaluated. These findings are in agreement with the data of Montagna et al from Italy [ 32 ], Tseng et al from Taiwan [ 33 ], and Ganeshkumar et al from India [ 34 ]. Lovero et al showed that Etest is a reliable method for determining the susceptibility of Candida species to L-AMB [ 35 ].…”

Section: Discussionsupporting

confidence: 93%

Analysis of Biofilm-Related Genes and Antifungal Susceptibility Pattern of Vaginal Candida albicans and Non-Candida albicans Species

Mohammadi

Hemmat

Bajalan

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

Background. Vulvovaginal candidiasis caused by Candida species is a prevalent fungal infection among women. It is believed that the pathogenesis of Candida species is linked with the production of biofilm which is considered a virulence factor for this organism. The aim of this study was molecular identification, antifungal susceptibility, biomass quantification of biofilm, and detection of virulence markers of Candida species. Methods. We investigated the molecular identification of 70 vaginal isolates of Candida species, antifungal resistance to amphotericin B, fluconazole, itraconazole, and voriconazole according to CLSI M27-A3 and M27-S4, biofilm formation, and frequency analysis of biofilm-related ALS1, ALS3, and HWP1 genes. Results. Our findings showed that the most common yeast isolated from vaginal discharge was C. albicans (67%), followed by the non-Candida albicans species (33%). All C. albicans complex isolates were confirmed as C. albicans by HWP-PCR, and all isolates of the C. glabrata complex were revealed to be C. glabrata sensu stricto using the multiplex PCR method. FLC resistance was observed in 23.4% of C. albicans and 7.7% of C. glabrata. The resistance rate to ITC was found in 10.6% of C. albicans. The frequency of ALS1, ALS3, and HWP1 genes among Candida species was 67.1%, 80%, and 81.4%, respectively. Biofilm formation was observed in 54.3% of Candida species, and the highest frequency detected as a virulence factor was for the ALS3 gene (97.3%) in biofilm-forming species. Discussion. Our results showed the importance of molecular epidemiology studies, investigating antifungal susceptibility profiles, and understanding the role of biofilm-related virulence markers in the pathogenesis of Candida strains.

show abstract