Antigen‐Induced Inhibition of Autoimmune Response to Rat Male Accessory Glands

Ferro, Maria Elena; Galmarini, Mirian; Riera, Clelia M.

doi:10.1111/j.1600-0897.1985.tb00280.x

Cited by 19 publications

(5 citation statements)

References 21 publications

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“…in the soluble form before the immunization with MRAG-CFA [7]. The unresponsiveness is specific for the autoantigen at both the cellular and humoral level.…”

Section: Discussionmentioning

confidence: 99%

“…Several reports [2- In previous studies we have developed an experimental model of suppression of autoimmune response to chemically modified rat male accessory gland saline extract (MRAG) by i.p. administration of low doses of fraction I (Sephadex G-100) FI of RAG prior to the immunization with MRAG in CFA.The unresponsiveness was specific for the autoantigen at both the cellular and humoral levels [7]. The cell types involved in the suppression included MRAGspecific, cyclophosphamide (a)-sensitive, inducer-phase T, cells.…”

Section: Introductionmentioning

confidence: 98%

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Antigen‐induced inhibition of autoimmune response to rat male accessory glands: distinct characteristics of I‐A‐ and I‐E‐positive peritoneal cells

et al. 1991

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The present report describes different aspects of two populations of peritoneal cells (PC) obtained from rats injected i.p. 2 h or 24 h previously with a suppressor dose of a purified fraction (FI) of rat male accessory glands (RAG) (FI-PC2h and FI-PC24h, respectively). The FI-PC2h, which are mainly I-E (OX17) positive and can suppress the autoimmune response to RAG autoantigens, have an elevated phagocytic activity against Candida albicans and capacity to reduce the dye nitroblue tetrazolium. In contrast, FI-PC24h, which are mainly I-A (OX6) positive and can potentiate the autoimmunity to RAG autoantigens, have a diminished capacity to reduce the dye and a diminished phagocytic activity. Moreover, the Toxoplasma gondii appear to have a different effect on both populations. The parasites can invade FI-PC2h while FI-PC24h offer resistance to T. gondii aggression. FI-PC2h cultured during 22 h (FI-PC2-24h in vitro), or PC obtained from syngeneic recipients injected i.p. 22 h previously with FI-PC2h (FI-PC2-24h in vivo) show, as FI-PC2h, an increase of the I-E+ cells and capacity to induce suppression of the delayed-type hypersensitivity response to RAG autoantigens when they are injected to syngeneic rats 10 and 3 days prior to the immunization with chemically modified (diazotized arsanilic and sulfanilic acid) RAG in complete Freund's adjuvant. The PC obtained 24 h after injection of irradiated rats with N-PC plus FI show an increase of I-E+ cells whereas an enhancement of I-A+ cells can be observed when the PC are obtained 24 h after injection of irradiated and bone marrow-reconstituted rats with N-PC plus FI. These findings appear to indicate that FI-PC2h and FI-PC24h are functionally different and that the population obtained 24 h after injection of FI of RAG could not originate from either the population present 2 h after injection of FI of RAG injection nor from normal PC. They appear to require bone marrow precursors.

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“…in the soluble form before the immunization with MRAG-CFA [7]. The unresponsiveness is specific for the autoantigen at both the cellular and humoral level.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Antigen‐induced inhibition of autoimmune response to rat male accessory glands: distinct characteristics of I‐A‐ and I‐E‐positive peritoneal cells

et al. 1991

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“…Using another experimental model of autoimmunity against rat male sex accessory glands induced by immunization with a chemically modified accessory gland saline extract (MRAG) in complete Freund's adjuvant (CFA), we have previously shown that a purified fraction of rat male accessory glands intraperitoneally administered in low doses and soluble form prevents the cellular and humoral autoimmune response when animals are subsequently challenged with MRAG in CFA (Ferro et al , 1985(Ferro et al , , 1990Rivero et al, 199 1). Application of a similar experimental model to EAE demonstrated inhibition of the clinical signs of the disease with a concurrent suppression of the proliferative response of mononuclear cells to MBP (Rivero et al, 1995(Rivero et al, , 1996.…”

Section: Introductionmentioning

confidence: 99%

Experimental autoimmune encephalomyelitis: Antigen-induced inhibition of biochemical and immunohistological alterations

et al. 1996

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A comprehensive biochemical, immunological, and histological study was undertaken during suppression of experimental autoimmune encephalomyelitis (EAE) induced by antigen‐specific inhibition of the immune response. Pretreatment of Wistar rats by intraperitoneal administration of low doses of saline‐soluble bovine myelin or myelin basic protein (MBP) but not with ovalbumin suppresses the appearance of the clinical symptoms of EAE induced by sensitization with bovine myelin in complete Freund's adjuvant. Analysis of the central nervous system (CNS) of animals pretreated with MBP or whole myelin shows inhibition of the diminution of MBP and 2′,3′‐cyclic nucleotide 3′‐phosphohydrolase (CNPase) activity observed in the EAE animals or in rats pretreated with ovalbumin. With respect to the CNS lipid content, these suppressive treatments abolish the increase in esterified cholesterol and partially revert the diminution in the content of cerebrosides and total cholesterol characteristic of the acute stage of the disease. Concomitantly, meningeal and parenchymal infiltration with mononuclear cells and deposits of immunoglobulins in the infiltrated regions as well as in spinal cord motor neurons were reduced. Analysis of the humoral response to myelin antigens shows that all EAE as well as treated animals developed antibodies to MBP and other myelin proteins. However, a higher incidence and level of these antibodies was observed in nontreated EAE animals and MBP‐ and ovalbumin‐treated rats, while rats treated with total bovine myelin showed a highly reduced humoral response. The present results indicate that intraperitoneal treatment with soluble forms of myelin antigens, concomitant with the suppression of the clinical symptoms of the disease, markedly reduces the biochemical and histological alterations occurring in EAE animals and produces changes in the autoimmune humoral response. © 1996 Wiley‐Liss, Inc.

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“…Taking into account that the relationship of the senescent immunosuppressor system to the aging process remains poorly understood, in this present report we analyze the suppressor autoimmune system using an experimental model of suppression of autoimmunity to MRAG developed in our laboratory [6]. In fact, a state of specific tolerance to MRAG was induced when low doses of purified fractions of RAG were injected i.p.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we have analyzed the ability to induce suppression to RAG autoantigens and the characteristics of TJnducer peritoneal cells (PC) of old rats using an experimental model of suppression of autoimmunity to RAG [6,7], in which antigen-specific inducer-phase T, cells, effector-phase suppressor cells and I-E+ APC are involved.…”

Section: Introductionmentioning

confidence: 99%

Effect of aging on the autoimmune response to rat male accessory glands: deficit of I‐E‐positive peritoneal cells capable of inducing suppression

1991

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The present report analyzes the ability to induce suppression to rat male accessory gland (RAG) autoantigens and the characteristics of T suppressor (Ts)-inducer peritoneal cells (PC) in old rats which show increased autoimmune responses. The injection of young rats with a purified fraction (FI) of RAG 10 and 3 days prior to immunization with chemically modified RAG (MRAG) markedly reduced the immune response to RAG autoantigens when compared with young rats which had only been immunized (controls), while the pretreatment of old rats did not block the delayed-type hypersensitivity reaction to MRAG when compared with control old rats. The study of cell surface markers on PC from rats injected i.p. 2 h previously with FI of RAG (FI-PC) showed an increase of OX-6 (I-A) and a decrease of OX-17 (I-E) in FI-PC of old rats with respect to FI-PC of young animals, which showed a selective increase of I-E+ Ts-inducer PC. The i.p. injection of FI-PC from old rats into young recipients, 10 and 3 days prior to immunization with MRAG in complete Freund's adjuvant, did not modify the autoimmune response when compared with controls. By contrast, the injection of young and old rats with FI-PC from young animals induced a significant suppression of the autoimmune response. The reduced percentage of I-E+ suppressor-inducer PC provides an explanation for the diminished ability to induce suppression to RAG autoantigens in old rats.

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Antigen‐Induced Inhibition of Autoimmune Response to Rat Male Accessory Glands

Cited by 19 publications

References 21 publications

Antigen‐induced inhibition of autoimmune response to rat male accessory glands: distinct characteristics of I‐A‐ and I‐E‐positive peritoneal cells

Antigen‐induced inhibition of autoimmune response to rat male accessory glands: distinct characteristics of I‐A‐ and I‐E‐positive peritoneal cells

Experimental autoimmune encephalomyelitis: Antigen-induced inhibition of biochemical and immunohistological alterations

Effect of aging on the autoimmune response to rat male accessory glands: deficit of I‐E‐positive peritoneal cells capable of inducing suppression

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