Antigen presentation using novel particulate organelles from halophilic archaea

Stuart, Elizabeth S.; Morshed, Fazeela; Sremac, Marinko; DasSarma, Shiladitya

doi:10.1016/s0168-1656(01)00267-x

Cited by 50 publications

(83 citation statements)

References 31 publications

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“…Several additional gvp gene products beyond the seven identified thus far are also likely to be involved in gas vesicle formation. With the availability of immunological probes, genome sequence, expression vectors, and gene knockout and replacement methodology (5,24,27,31), we now have in place many tools necessary for better understanding the process of gas vesicle biogenesis in the model archaeon, Halobacterium sp. strain NRC-1.…”

Section: Resultsmentioning

confidence: 99%

Complexity of Gas Vesicle Biogenesis inHalobacteriumsp. Strain NRC-1: Identification of Five New Proteins

Shukla

DasSarma

2004

J Bacteriol

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The genome of Halobacterium sp. strain NRC-1 contains a large gene cluster, gvpMLKJIHGFEDACNO, that is both necessary and sufficient for the production of buoyant gas-filled vesicles. Due to the resistance of gas vesicles to solubilization, only the major gas vesicle protein GvpA and a single minor protein, GvpC, were previously detected. Here, we used immunoblotting analysis to probe for the presence of gas vesicle proteins corresponding to five additional gvp gene products. Polyclonal antisera were raised in rabbits against LacZGvpF, -GvpJ, and -GvpM fusion proteins and against synthetic 15-amino-acid peptides from GvpG and -L. Immunoblotting analysis was performed on cell lysates of wild-type Halobacterium sp. strain NRC-1, gas vesicle-deficient mutants, and purified gas vesicles, after purification of LacZ fusion antibodies on protein A and ␤-galactosidase affinity columns. Our results show the presence of five new gas vesicle proteins (GvpF, GvpG, GvpJ, GvpL, and GvpM), bringing the total number of proteins identified in the organelles to seven. Two of the new gas vesicle proteins are similar to GvpA (GvpJ and GvpM), and two proteins contain predicted coiled-coil domains (GvpF and GvpL). GvpL exhibited a multiplet ladder on sodium dodecyl sulfate-polyacrylamide gels indicative of oligomerization and self-assembly. We discuss the possible functions of the newly discovered gas vesicle proteins in biogenesis of these unique prokaryotic flotation organelles.

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Section: Resultsmentioning

confidence: 99%

Complexity of Gas Vesicle Biogenesis inHalobacteriumsp. Strain NRC-1: Identification of Five New Proteins

Shukla

DasSarma

2004

J Bacteriol

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“…Epitope display experiments imply that the C terminus of GvpA might be situated on the cytosolic side of the gas vesicle in Halobacterium sp. NRC-1 [11,12] and the unusual properties of actinomycete C-terminal GvpA could hint towards alternative functions for gas vesicles in actinomycetes.…”

Section: Gas Vesicle Genes In Other Actinomycetesmentioning

confidence: 99%

Gas vesicles in actinomycetes: old buoys in novel habitats?

Keulen

Hopwood

Dijkhuizen

et al. 2005

Trends in Microbiology

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“…Because gas vesicles make cells float, they have been proposed as a tool to facilitate harvests of bacteria in an industrial setting through the formation of a pellicle. Purified gas vesicles can also be used to display antigenic peptides (Stuart et al 2001), to oxygenate tissue culture media (Sundararajan and Ju 2006), and to serve as contrast agents for ultrasound imaging (Shapiro et al 2014).…”

Section: Gas Vesiclesmentioning

confidence: 99%

Building Spatial Synthetic Biology with Compartments, Scaffolds, and Communities

Polka

Hays

Silver

2016

Cold Spring Harb Perspect Biol

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Traditional views of synthetic biology often treat the cell as an unstructured container in which biological reactions proceed uniformly. In reality, the organization of biological molecules has profound effects on cellular function: not only metabolic, but also physical and mechanical. Here, we discuss a variety of perturbations available to biologists in controlling protein, nucleotide, and membrane localization. These range from simple tags, fusions, and scaffolds to heterologous expression of compartments and other structures that confer unique physical properties to cells. Next, we relate these principles to those guiding the spatial environments outside of cells such as the extracellular matrix. Finally, we discuss new directions in building intercellular organizations to create novel symbioses.

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Antigen presentation using novel particulate organelles from halophilic archaea

Cited by 50 publications

References 31 publications

Complexity of Gas Vesicle Biogenesis inHalobacteriumsp. Strain NRC-1: Identification of Five New Proteins

Complexity of Gas Vesicle Biogenesis inHalobacteriumsp. Strain NRC-1: Identification of Five New Proteins

Gas vesicles in actinomycetes: old buoys in novel habitats?

Building Spatial Synthetic Biology with Compartments, Scaffolds, and Communities

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