2002
DOI: 10.1016/s0035-9203(02)90119-7
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Antigen-specific activation and proliferation of CD4+ and CD8+ T lymphocytes from brucellosis patients

Abstract: Salt-extractable antigen from Brucella melitensis 16M (RCM-BM) was used to evaluate the immune response from acute and chronic patients suffering from Brucella infections (in Mexico); their responses were compared with those of healthy controls. As a readout we used upregulation of CD69 (a well-established early activation marker for lymphocytes), lymphocyte proliferation by 3[H]thymidine or 5-bromo-2-deoxyuridine (BrdU) incorporation measured by liquid scintillation or flow cytometry, respectively, and produc… Show more

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Cited by 28 publications
(16 citation statements)
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“…In humans, it has been demonstrated that T cells from patients with brucellosis produce IFN- when stimulated in vitro with Brucella antigens. This production by CD8+T cells is higher during the acute stage of the disease (Moreno et al, 2002). In the present study we observed that PEC from B. abortus RB51-vaccinated mice expressed the genes that encode cytokines IL-6, IL-12, TNF-α, IFN-, and iNOS.…”
Section: Discussionsupporting
confidence: 57%
“…In humans, it has been demonstrated that T cells from patients with brucellosis produce IFN- when stimulated in vitro with Brucella antigens. This production by CD8+T cells is higher during the acute stage of the disease (Moreno et al, 2002). In the present study we observed that PEC from B. abortus RB51-vaccinated mice expressed the genes that encode cytokines IL-6, IL-12, TNF-α, IFN-, and iNOS.…”
Section: Discussionsupporting
confidence: 57%
“…24 Accordingly, granulysin has the potential to play a critical role in the defense against tumor growth and microbial infection. An extension of this observation reveals that increased expression of granulysin by CD4 ϩ CTLs correlated with their cytotoxicity to viral and M tuberculosis-infected cells or M leprae-Ag-pulsed macrophages [36][37][38][64][65][66] and that strontium ions abrogated the antimicrobial activity. 38,67,68 However, SrCl 2 depletes all of the granule components without molecular specificity, and consequently none of these studies has demonstrated a direct role of granulysin in cellular cytotoxicity.…”
Section: Discussionmentioning
confidence: 81%
“…These observations suggest that once acute brucellosis has resolved, that both the number of CD4+ cells and CD4+ functional response is reduced. Whether antigen-specific memory CD4+ cells are produced during acute or chronic brucellosis remains to be determined (Moreno-Lafont et al, 2002; Akbulut et al, 2005; Kinikli et al, 2005). Recently Elfaki and Al-Hokail observed that mice deficient in β2-microglobulin produced an impaired CD8+ response associated with increased Brucella bacterial load and decreased clearance (Moreno-Lafont et al, 2002; Elfaki and Al-Hokail, 2009).…”
Section: T Cell Cytokine Responses To Brucella Infectionmentioning
confidence: 99%