Antigenic analysis of bean pod mottle virus using linear and cyclized synthetic peptides

Abstract

The antigenic structure of the comovirus bean pod mottle virus (BPMV) was studied using synthetic peptides selected on the basis of the exposed location of certain regions of the viral protein. Three regions of domain A, four regions of domain B and two regions of domain C of BPMV coat protein were studied. Each of four regions were synthesized in the form of linear and cyclized peptides while the others were synthesized as linear peptides only. The peptides were tested for their ability to be recognized by antibodies directed against BPMV. The peptides were also used for producing rabbit antisera, which were tested for their ability to react with various BPMV antigens as well as with the linear and cyclized peptides. All the peptides were found to correspond to epitopes of BPMV coat protein. Several of the antigenic sites of BPMV located on exposed loops of the coat protein occupy positions which correspond to known epitopes in the structurally related picornaviruses. Only in some cases did cyclization sufficiently improve the level of conformational mimicry between peptides and the viral protein to allow cross-reactions between them to be observed.

Analysis of antigenic structure of Potato Virus M Ukrainian strains

“…MAb M6D5 recognized four peptides, while MAb M9G1 reacted only with the two short peptides. Other investigators [19,20] have observed a similar phenomenon, when antibody re cognised a shorter peptide, but not longer one. On the basis of these ELISA results we assumed that, first, MAbs M6D5 and M9G1 recognise two overlapping epitopes (M6D5 epitope and M9G1 epitope, res pectively).…”

Antigenicity modulation upon peptide cyclization: application to the GH loop of foot-and-mouth disease virus strain C1-Barcelona

“…The usefulness of cyclization as a means to enhance the antigenicity of peptides that, in the linear form, do not suitably reproduce the antigenic behavior of the native antigen has been generally acknowledged [14,[29][30][31][32][33]. In this work, we have successfully applied the cyclization approach to the GH loop of FMDV C-S30 and found an improvement of two orders of magnitude in the recognition by mAb 4C4 relative to the linear form.…”

Oligonucleotide microarray with a minimal number of probes for the detection and identification of thirteen genera of plant viruses

“…BPMV may cause up to 85% loss in mixed infection with Soybean mosaic virus (SBMV) (Giesler et al, 2002). BPMV is an important official quarantine virus in China and can be detected by ELISA and RT-PCR (Gu et al, 2002;Joisson et al, 1993), but no microarray-based method has been reported for its detection. Thus Comovirus was studied in this investigation.…”