Antigenic analysis of genetic variants of Canine distemper virus

Anis, Eman; Holford, Amy; Galyon, Gina D.; Wilkes, Rebecca P.

doi:10.1016/j.vetmic.2018.03.014

Cited by 33 publications

(29 citation statements)

References 29 publications

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“…These sites are also close to or within epitopes of presentation to B lymphocytes reported by Li et al (2018), and due to the high divergence in these epitopes, the monoclonal antibodies found in this study can differentiate between vaccination and wild-type strains [23]. Considering these results together, it is possible to postulate that changes in this gene also contribute to the low or null production of vaccine antibodies capable of neutralizing wild-type strains (humoral response), as reported for the strains characterized in the United States belonging to the South America/North America-4 Lineage, or may even be one of the explanations for viral immune escape strains [24,25].…”

Section: Discussionsupporting

confidence: 65%

Phylogenomic Analysis of Two Co-Circulating Canine Distemper Virus Lineages in Colombia

2019

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Canine distemper virus (CDV) is considered a reemerging disease-causing agent in domestic dogs because it presents high divergence among circulating strains worldwide. In Colombia, the South America-3 and South America/North America-4 lineages co-circulate in domestic dogs, both in the Medellin metropolitan area. In this paper, two full CDV genomes from each viral lineage circulating in Medellin were sequenced; we explored the phylogenetic relationship with the available genome sequences; we described the presence of CDV mutations in the South America-3 and South America/North America-4 lineages associated with adaptation to human cells and a crossing of the species barrier and pathogenicity; and we established the evolutionary rates and time of the closest common ancestor for each gene and characterized the presentation of multiple genomic sites by positive selection.

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Section: Discussionsupporting

confidence: 65%

Phylogenomic Analysis of Two Co-Circulating Canine Distemper Virus Lineages in Colombia

2019

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“…CDV is presently recognized as a single serotype 47 as there is little evidence of antigenic divergence as a result of genetic divergence. Recently, significant differences were reported in the evaluation of neutralizing titers between “South America/North America-4” lineage strains and an America-1 type vaccine strain 27 . Given those results and the fact that multiple recognized CDV cases have been recorded in vaccinated animals 17,21,28,48 , it is necessary to perform wider, updated antigenic analyses of CDV for understanding the antigenic differences between the multiple worldwide circulating lineages and, potentially, to produce a vaccine update that includes most prevalent antigenic types.…”

Section: Discussionmentioning

confidence: 99%

“…Although immunization with attenuated vaccines has been widely used for preventing CDV, researchers have hypothesized that the strong genetic diversity and wide variability of the H gene could mean that the antigenic profile of these new genetic variants is altered relative to the vaccine strain if specific sites on the H protein associated with immune neutralization are affected 9,25,26 . In fact, recent analysis has suggested the need for developing an updated CDV vaccine due to differences in cross-neutralization assays revealing wide antigenic differences among wild-type CDV isolates and the vaccine strains currently used in the U.S. 27 .…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic evidence of the intercontinental circulation of a Canine distemper virus lineage in the Americas

Duque-Valencia

Forero-Muñoz²,

Díaz

et al. 2019

Sci Rep

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Canine distemper virus (CDV) is the cause of a multisystem disease in domestic dogs and wild animals, infecting more than 20 carnivore and non-carnivore families and even infecting human cell lines in in vitro conditions. Phylogenetic classification based on the hemagglutinin gene shows 17 lineages with a phylogeographic distribution pattern. In Medellín (Colombia), the lineage South America-3 is considered endemic. Phylogenetic studies conducted in Ecuador using fragment coding for the fusion protein signal peptide (Fsp) characterized a new strain belonging to a different lineage. For understanding the distribution of the South America-3 lineage in the north of the South American continent, we characterized CDV from three Colombian cities (Medellín, Bucaramanga, and Bogotá). Using phylogenetic analysis of the hemagglutinin gene and the Fsp region, we confirmed the circulation of CDV South America-3 in different areas of Colombia. We also described, for the first time to our knowledge, the circulation of a new lineage in Medellín that presents a group monophyletic with strains previously characterized in dogs in Ecuador and in wildlife and domestic dogs in the United States, for which we propose the name “South America/North America-4” due its intercontinental distribution. In conclusion, our results indicated that there are at least four different CDV lineages circulating in domestic dogs in South America: the Europe/South America-1 lineage circulating in Brazil, Uruguay, and Argentina; the South America-2 lineage restricted to Argentina; the South America-3 lineage, which has only been reported in Colombia; and lastly an intercontinental lineage present in Colombia, Ecuador, and the United States, referred to here as the “South America/North America-4” lineage.

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“…And the antigenic differences between vaccine strains and wild-type strains have been reported. Due to antigenic differences between the vaccine strains and the currently circulating wildtype strains, current vaccines may provide incomplete protection (15). So it's necessary to clarify the detailed sequence of linear B-cell epitopes of the CDV H protein.…”

Section: Discussionmentioning

confidence: 99%

Identification of Linear B-Cell Epitopes on Hemagglutinin Protein of Canine Distemper Virus Using Two Monoclonal Antibodies

et al. 2020

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Canine distemper virus (CDV) belongs to the Morbillivirus genus of the Paramyxoviridae family, which causes a threat to the domestic dog and fur-animal industry. Hemagglutinin protein is a major membrane protein of the vital molecular factor in CDV tropism, also known to induce hosts to produce neutralizing antibodies. In the current study, we prepared two monoclonal antibodies, 1A5 and 2B8, against the H protein of the CDV-PS strain. A series of partially overlapping synthetic peptides covering the hemagglutinin protein (amino acids 50-204) were screened to define the linear epitope identified by 1A5 and 2B8 mAbs. 120 QKTNFFNPNREFDFR 134 (F8) and 178 ARGDIFPPY 186 (F14-1) are minimal linear epitopes recognized by 1A5 and 2B8 mAbs, respectively. Further investigations revealed that F8 is conserved in different CDV strains; however, F14-1 contains mutant residues 178, 179, and 180. The epitopes F8 and F14-1 localized at the surface of hemagglutinin protein in a three-dimensional (3D) structure. CDV-infected dog serum can also recognize the identified B-cell epitopes.

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Antigenic analysis of genetic variants of Canine distemper virus

Cited by 33 publications

References 29 publications

Phylogenomic Analysis of Two Co-Circulating Canine Distemper Virus Lineages in Colombia

Phylogenomic Analysis of Two Co-Circulating Canine Distemper Virus Lineages in Colombia

Phylogenetic evidence of the intercontinental circulation of a Canine distemper virus lineage in the Americas

Identification of Linear B-Cell Epitopes on Hemagglutinin Protein of Canine Distemper Virus Using Two Monoclonal Antibodies

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