Antigenic analysis of iron-regulated proteins in Pasteurella haemolytica A and T biotypes by immunoblotting reveals biotype-specific epitopes

Je, Murray; Rc, Davies; Fa, Lainson; Cf, Wilson; Donachie, W.

doi:10.1099/00221287-138-2-283

Cited by 15 publications

(10 citation statements)

References 16 publications

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“…An alternative approach to limit the levels of iron is to use a complex medium that has been treated with various amounts of an iron chelator. Several iron chelators have been used in ironlimitation studies, including ethylenediamine di(ohydroxyphenylacetic acid) (EDDA), 2,2-dipyridyl (BPD), and Chelex 100 (Strathdee and Lo, 1989;Murray et al, 1992;Winston et al, 1993). BPD was chosen for our experiments because Chelex 100 is less specific and because EDDA does not work effectively in the anaerobic conditions used for the growth of A. actinomycetemcomitans.…”

Section: The Effect Of Iron Restriction On Leukotoxin Levelsmentioning

confidence: 99%

“…For example, the pH of the crevicular fluid is reported to be higher than that of normal human serum (Bickel et al, 1985 has become clear that most human pathogens must adapt to limited amounts of "available" iron in the human host (Mekalanos, 1992). To compensate, many organisms, including A. actinomycetemcomitans and other periodontal pathogens, increase the synthesis of iron-acquisition proteins and other virulence factors in response to iron limitation (Grunig and Lebek, 1988;Holt and Bramanti, 1991;Mekalanos, 1992;Murray et al, 1992;Winston et al] 1993). ' While major advances have been made in determining the mechanisms of regulation of virulence factors in many microorganisms, comparatively little is known about regulation in periodontal pathogens.…”

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confidence: 99%

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The Regulation of Leukotoxin Production in Actinobacillus Actinomycetemcomitans Strain JP2

Spitznagel

Kraig²,

Kolodrubetz³

1995

Adv Dent Res.

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Actinobacillus actinomycetemcomitans (A.a.) can produce a potent leukotoxin that is thought to be involved in evasion of the host immune response. In order to understand the role of A.a. and its leukotoxin in the initiation and progression of periodontal disease, it is important determine how the expression of A.a. virulence factors might be regulated by the local periodontal micro-environment. To facilitate the measurement of leukotoxin levels, a leukotoxin-beta-galactosidase gene fusion was constructed and recombined into the chromosome of A.a. strain JP2 at the leukotoxin locus. The resulting strain, AAM17, produces beta-galactosidase under control of the leukotoxin promoter. It also produces leukotoxin, since integration of the gene fusion into the chromosome was designed to produce a duplication of the leukotoxin gene. This strain was used to measure the change in leukotoxin level in response to alterations in two environmental signals: iron concentration and oxygen tension. When AAM17 was grown in iron-limited media that did not alter growth rate but did increase the levels of other iron-regulated proteins, the levels of the leukotoxin-beta-galactosidase were similar to those found in AAM17 grown in iron-replete media. These results were confirmed in strains AAM17 and JP2 by leukotoxicity assays and RNA blots. Aerobic growth of AAM17 resulted in a three-fold decrease in leukotoxin beta-galactosidase activity compared with anaerobically grown cells. These results indicate that the A.a. leukotoxin is regulated by some of the environmental signals that may vary in the gingival crevice.

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Section: The Effect Of Iron Restriction On Leukotoxin Levelsmentioning

confidence: 99%

mentioning

confidence: 99%

The Regulation of Leukotoxin Production in Actinobacillus Actinomycetemcomitans Strain JP2

Spitznagel

Kraig²,

Kolodrubetz³

1995

Adv Dent Res.

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“…Additionally, some of these vaccines have, in principle, been designed for cattle, where the most prevalent M. haemolytica serotype is A1 (Odendaal and Henton, 1995), thus they confer reduced protection against serotypes of the organisms of pathogenic significance in sheep (Purdy et al, 1998). Vaccines manufactured with a more recent technology, based on iron-regulated proteins (Gilmour et al, 1991;Murray et al, 1992) can offer better protection and can afford full protection independently of serotype.…”

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confidence: 99%

Mannheimia haemolytica and Bibersteinia trehalosi serotypes isolated from lambs with ovine respiratory complex in Spain

González¹,

Lacasta

Ferrer

et al. 2017

J Hellenic Vet Med Soc

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ABSTRACT. Ovine respiratory complex causes significant economic losses in ovine production systems around the world. Mannhemia haemolytica and Bibersteinia trehalosi are the two most frequently isolated bacterial species. In this study, bacterial isolates of M. haemolytica and B. trehalosi isolated from dead lambs were serotyped by means of indirect haemagglutination, with the general aim to improve information in the preparation for candidate strains for relevant vaccines. In total, 84 isolates were serotyped. Eight different M. haemolytica serotypes and four different B. trehalosi serotypes were identified. The most frequent M. haemolytica serotypes were A2 (29% of isolates), A12 (18%) and A1 (12%), while B. trehalosi were predominantly T4 (40% of isolates) and a group sharing characteristics of T3 and T15 serotypes (43%). Isolation of T4 serotype was associated with lack of consolidated lesions in lungs, whilst isolation of T3-T15 group was associated with lung consolidation. It is concluded that a potential vaccine to control the disease presented as a bacterin based on outer membrane proteins and leucotoxins of the organisms, it should contain the above, frequently implicated serotypes. Alternatively, vaccines based on iron-regulated proteins can be possible replacements.

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“…Human infections with P. multocida frequently are connected with animal bites and infected 315 wounds, human P. haemolytica infections are rare. Several outer membrane proteins are induced in P. haemolytica under iron-restricted conditions, which vary in size among the various serotypes [114]. In clinical P. muhocida isolates associated with bovine pneumonia, no siderophores could be detected, but transferrin was able to stimulate growth of these bacteria.…”

Section: Pasteurella Multocida P Haemolyticamentioning

confidence: 99%

Signal transduction and virulence regulation in human and animal pathogens

Gross¹

1993

FEMS Microbiology Letters

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Pathogens have developed many strategies for survival in animals and humans which possess very effective defense mechanisms. Although there are many different ways, in which pathogenic bacteria solved the problem to overcome the host defense, some common features of virulence mechanisms can be detected even in phylogenetically very distant bacteria (Finlay and Falkow (1989) Microb. Rev. 6 1375–1383). One important feature is that the regulation of expression of virulence factors and the exact timing of their expression is very important for many of the pathogenic bacteria, as most of them have to encounter different growth situations during an infection cycle, which require a fast adaptation to the new situation by the expression of different factors. This review gives an overview about the mechanisms used by pathogenic bacteria to accomplish the difficult task of regulation of their virulence potential in response to environmental changes. In addition, the relationship of these virulence regulatory systems with other signal transduction mechanisms not involved in pathogenicity is discussed.

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Antigenic analysis of iron-regulated proteins in Pasteurella haemolytica A and T biotypes by immunoblotting reveals biotype-specific epitopes

Abstract: The antigenic relationships of the iron-regulated proteins (IRPs)

Cited by 15 publications

References 16 publications

The Regulation of Leukotoxin Production in Actinobacillus Actinomycetemcomitans Strain JP2

The Regulation of Leukotoxin Production in Actinobacillus Actinomycetemcomitans Strain JP2

Mannheimia haemolytica and Bibersteinia trehalosi serotypes isolated from lambs with ovine respiratory complex in Spain

Signal transduction and virulence regulation in human and animal pathogens

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