Antigenic and molecular analyses of the variability of bovine respiratory syncytial virus G glycoprotein.

Prozzi, Deborah; Walravens, Karl; Langedijk, Johannes P. M.; Daus, F.; Kramps, J. A.; Letesson, Jean‐Jacques

doi:10.1099/0022-1317-78-2-359

Cited by 43 publications

(53 citation statements)

References 38 publications

(37 reference statements)

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“…Monoclonal antibodies against glycoprotein G are used to determine the antigenic variability of bovine respiratory viruses, and recently several studies have been published on this subject (Schrĳver et al, 1996a;Prozzi et al, 1997;Elvander et al, 1998;Larsen et al, 1998). The shortcoming of those studies is the determination of the reaction pa�ern of glycoprotein G with different mAb sets.…”

Section: Antigenic Variabilitymentioning

confidence: 99%

“…Those viruses react only with some mAb specific for A group, but with another reaction pa�ern than that of the representatives of the intermediate AB group. The knowledge of that fact together with the high homology of nucleotide and amino acid sequences of G protein (Mallipeddi and Samal, 1993;Schrĳver et al, 1996a,b;Prozzi et al, 1997;Elvander et al, Review Article Vet. Med.…”

Section: Antigenic Variabilitymentioning

confidence: 99%

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The antigenic and genetic variability of bovine respiratory syncytial virus with emphasis on the G protein

Valentová¹

2003

Vet. Med.

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Bovine respiratory syncytial virus (BRSV) and related human respiratory syncytial virus (HRSV) are major respiratory tract pathogens in calves and infants, respectively. Great attention is now paid to prevention of the disease caused by these agents. Glycoprotein G is the most variable viral protein and antigenic grouping of RSV isolates is based on distinct antigenic reactivity patterns determined with a set of G protein specific mAbs. Genetic variability of the G protein is used during epidemiology and epizootiology studies of HRSV and BRSV diseases, respectively. The constant genetic drift can be observed within G protein sequences. Both cell-mediated and antibody-mediated immune responses contribute to efficient protection against RSV infection. The neutralizing antibodies are induced by F and G proteins. The G protein fails to induce cytotoxic lymphocytes response and may causes aberrant Th2 response leading to enhancement of clinical symptoms in subsequently infected vaccines. The G as the most variable viral protein associated with immunopathologic effect is a critical factor in vaccine development.

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Section: Antigenic Variabilitymentioning

confidence: 99%

Section: Antigenic Variabilitymentioning

confidence: 99%

The antigenic and genetic variability of bovine respiratory syncytial virus with emphasis on the G protein

Valentová¹

2003

Vet. Med.

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“…The primers were selected from two essential genes (F and G) of the BRSV genome because both are enveloped by highly immunogenic proteins. The G protein is the most antigenically dissimilar protein among the BRSV strains (19), and for this reason is extensively used in comparative BRSV studies.…”

Section: -Brmentioning

confidence: 99%

Characterization of bovine respiratory syncytial virus isolated in Brazil

Arns

Campalans

Costa

et al. 2003

Braz J Med Biol Res

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This paper presents the first isolation of bovine respiratory syncytial virus in Brazil and its physicochemical, morphological and molecular characterization. The virus was isolated from 33 samples of nasotracheal secretions, successively inoculated into a Madin-Darby bovine kidney cell culture, which was characterized by physicochemical tests and morphological observation by electron microscopy. The Brazilian sample is an RNA pleomorphic, enveloped, thermolabile and nonhemagglutinating spicular virus. Reverse transcription, followed by nested polymerase chain reaction (nRT-PCR) assay was carried out using oligonucleotides B1, B2A, B3 and B4 for the fusion proteins (F) and B5A, B6A, B7A and B8 for the attachment protein (G). The nRT-PCR-F amplified a fragment of 481 bp corresponding to part of the gene that codes for protein F, whereas nRT-PCR-G amplified a fragment of 371 bp, in agreement with part of the G gene. The virus isolated from Brazilian samples in this study corresponded to the bovine respiratory syncytial virus, and RT-PCR proved to be useful for the diagnosis of bovine clinical samples.

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“…Probably the BRSV evolution varies according to the gene, being the G protein gene highly tolerant to fixation of mutations when compared to F (VALARCHER; SCHELCHER; BOURHY, 2000). Therefore, most studies concerning characterization of isolates were focused on this protein (PROZZI et al, 1997;SCHRIJVER et al, 1998;SPILKI et al, 2006;BIDOKHTI et al, 2012). Even though, progressive accumulation of nucleotide changes was reported for fragments of both genes (VALARCHER; SCHELCHER; BOURHY, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…The unclassified isolates were denominated as untyped (FURZE et al, 1994; VAN DER POEL et al, 1994;SCHRIJVER et al, 1996a;WOELK;HOLMES, 2001). The importance of this diversity regarding immunoprophylaxis is not yet completely understood, since it is not known whether vaccination against one BRSV subgroup induces cross-protection, as the natural infection ( VAN DER POEL et al, 1994;SCHRIJVER et al, 1996b).…”

Section: Introductionmentioning

confidence: 99%

Detection of an untyped strain of bovine respiratory syncytial virus in a dairy herd

et al. 2014

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Bovine respiratory syncytial virus (BRSV) causes important lower respiratory tract illness in calves. According to F and G proteins genetic sequences, three BRSV subgroups have been reported and characterized in several countries, showing differences in its distribution. In Brazil, the virus is widely disseminated throughout the herds and the few characterized isolates revealed the solely occurrence of the subgroup B. This study describes the detection and characterization of an untyped BRSV strain from a twenty-days-old calf from a herd without clinical respiratory disease. Nasal swabs were analyzed by RT-nested PCR for the F and G proteins genes. One sample has amplified the F protein gene. Sequencing and subsequent phylogenetic reconstruction were accomplished, revealing that the strain could not be grouped with any other BRSV subgroups reported. This result may suggest that the BRSV is in constantly evolution, even in Brazil, where the vaccination is not a common practice. More detailed studies about BRSV characterization are necessary to know the virus subgroups distribution among the Brazilian herds to recommend appropriated immunoprophylaxis. Key words: BRSV, F protein gene, RT-nested PCR, sequences, subgroup ResumoO vírus respiratório sincicial bovino (BRSV) é responsável por causar severa doença respiratória principalmente em bezerros. De acordo com sequências genéticas das proteínas F e G deste vírus, três subgrupos de BRSV foram relatados e caracterizados em vários países, mostrando diferenças nas suas distribuições. No Brasil, o vírus encontra-se disseminado pelos rebanhos bovinos e, dos poucos

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Antigenic and molecular analyses of the variability of bovine respiratory syncytial virus G glycoprotein.

Cited by 43 publications

References 38 publications

The antigenic and genetic variability of bovine respiratory syncytial virus with emphasis on the G protein

The antigenic and genetic variability of bovine respiratory syncytial virus with emphasis on the G protein

Characterization of bovine respiratory syncytial virus isolated in Brazil

Detection of an untyped strain of bovine respiratory syncytial virus in a dairy herd

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