Antigenic determinants of heavy chain variable regions: immunological typing of the human immunoglobulin VHIII subgroup

Abstract

An antigenic determinant of the VHIII variable region subgroup was defined by means of a heterologous specific antiserum using a hemagglutination inhibition procedure. The specificity of this antiserum was established in inhibition experiments with proteins either of known primary structure or belonging to a definite VH subgroup. A series of IgG, IgA, IgM and IgD monoclonal proteins was examined for the presence of this VHIII subgroup antigenic determinant. The data showed that 50% of the IgG, 62% of the IgA, 55% of the IgM and 41% of the IgD were VHIII-positive, and that certain "blocked" monoclonal immunoglobulins belonged to this subgroup. A preferential association of the VHIII antigenic determinant with the IgG1 and IgG3 subclasses was observed among IgG myeloma proteins while the preferential association was only observed with the IgG1 subclass when anti-Rh antibodies were studied. The VHIII subgroup exhibited nonallelic behavior.

Monoclonal immunoglobulins in patients with carcinoma of the colon

“…The heavy chains of proteins Shm, Slo, Dov, and Yos had pyrrolidonecarboxylic acid (PCA)-blocked NHz-termini. Serological typing using a VHIII/anti-VnIII hemagglutination inhibition system [25] indicated that the Shm heavy chain was VHIII, whereas the other three were non-VHIII.…”

Influence of IgG Subclasses on Automated Anti-D (Rh(0)) Quantification

“…Fur thermore, anti-D sensitized RBC were tested with the following antiglobulin sera: antiIgG4, anti-IgA, anti-IgAl, anti-IgA2 and anti-IgM. Antisera preparation and typing methods were previously described [9], All these tests were negative.…”