Antigenic Differences Among Human Haptoglobins

Subtyping Haptoglobins with Precipitating Antisera

“…Therefore sera of Hp 1-2 type were subjected to microimmunoclectrophoresis in starch gel. The fast component of Hp 1-2 which according to several authors [15,23] reacts immunologically like Hp 1-1, could be separated from the slower Hp 2-2 specific components. Figures 8 and 9 show typical experiments.…”

“…K o r n g o l d was the first to detect immunological differences between normal haptoglobins [23]. He used rabbit antisera against purified haptoglobin Hp 2-2.…”

“…This was carried out as described by Korngold [23] Immunoelectrophoresis. This was performed according to Sciii idkggkk [33] with 1 % agar gel in veronal-Na veronal-calcium lactate buffer [20].…”

Immunogenetics of human haptoglobins

“…The ability of these sera to recognize the antigenic determinants (A), (B), and (C) by Ouchterlony double diffusion technique is summarized in Table I. The antisera types I and III were the generous gift of Dr. Leonhard Korngold and have been described in detail by him (7,8).…”

“…Early studies on the immunologic characteristics of Hp did not reveal differences among the three phenotypes, or between free and Hb-bound Hp (5,6). Recently, however, with the use of more discriminating antiReceived for publication 2 May 1968. sera both these categories of antigenic differences have been demonstrated by Korngold (7,8) and by Eichmann, Deicher, and Cleve (9). The latter group has proposed the following terminology: (A) represents the antigenic determinant present in all common Hp types and in their Hb complexes.…”