Antigenic N to H conversion of poliovirus by a monoclonal antibody at low ionic strength

Delaet, Ingrid; Vrijsen, Raphael; Boeyé, Albert

doi:10.1016/0042-6822(92)90738-b

Cited by 8 publications

(17 citation statements)

References 20 publications

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“…Mixtures of labelled N antigen and N antigenspecific MAbs were incubated in PBS-NP40 (137 mM-NaC1, 2.7 mM-KC1, 9"5 mM-sodium phosphate, 0"05 % NP40, pH 7.3) at either 37 °C or 39 °C, or in low ionic strength buffer (LISB; 0-6raM-sodium phosphate, 0.1 mM-EDTA, 0-05 % NP40, pH 7.3) at 37 °C. The reaction in PBS-NP40 was stopped by cooling the mixture on ice: and that in LISB was stopped by raising the ionic strength to g = 0.16 with 10-fold concentrated PBS (Delaet et al, 1992). The MAbs were then removed by precipitation with Protein A-bearing fixed staphylococci, and the amounts of H antigen and residual N antigen in the supernatant were determined using anti-N antigen and anti-H antigen MAbs (Delaet et al, 1992).…”

Section: Methodsmentioning

confidence: 99%

“…The reaction in PBS-NP40 was stopped by cooling the mixture on ice: and that in LISB was stopped by raising the ionic strength to g = 0.16 with 10-fold concentrated PBS (Delaet et al, 1992). The MAbs were then removed by precipitation with Protein A-bearing fixed staphylococci, and the amounts of H antigen and residual N antigen in the supernatant were determined using anti-N antigen and anti-H antigen MAbs (Delaet et al, 1992). The antigenic conversion was taken as the radioactivity bound by the anti-H antigen antibody, expressed as a percentage of the total input radioactivity.…”

Section: Methodsmentioning

confidence: 99%

“…When the antibody is immobilized on Protein A-bearing bacteria this yield is lowered to 0'7 to 0.8 virions per antibody molecule (Delaet et al, 1992). When the immobilized antibody (IMAb) is incubated in LIS37 with excess virus, it loses its capacity to effect the antigenic conversion.…”

Section: Introductionmentioning

confidence: 99%

“…When the immobilized antibody (IMAb) is incubated in LIS37 with excess virus, it loses its capacity to effect the antigenic conversion. However, it regains this capacity when heated at 56 °C (Delaet et al, 1992). This paper presents a detailed comparison of the disruptive mechanisms in NIS39 and LIS37.…”

Section: Introductionmentioning

confidence: 99%

“…that of circulating blood, g = 0.16) at 37 °C (NIS37) this antibody neutralizes type 1 poliovirus by aggregation (Brioen et al, 1983 ;Thomas et al, 1985). Disruptive neutralization can be induced either by raising the temperature to 39 °C (NIS39; Delaet & Boey~, 1993) or, at 37 °C, by lowering the ionic strength to ~t = 0-002 (LIS37; Brioen et al, 1985;Delaet et al, 1992). Under these conditions, neutralization is irreversible, because the RNA is expelled from the virions, leaving an antigenically modified empty capsid (Brioen et al, 1985;Delaet & Boey~, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Capsid destabilization is required for antibody-mediated disruption of poliovirus

Delaet

Boeyé

1994

Journal of General Virology

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Three out of 36 poliovirus type 1-specific monoclonal antibodies which, at 37 °C in a medium of normal ionic strength (It = 0'16), caused only aggregative neutralization (reversible by immune complex dissociation at pH2) shifted to cause disruptive, acid-irreversible neutralization when the temperature was raised to 39 °C or the ionic strength was lowered to 1/100 of normal.Under both conditions, the antigenic conversion was stoichiometric, but the efficiency was lower at 39 °C than at low ionic strength. Antigenic conversion and irreversible neutralization under both conditions were inhibited by WIN 51711, a capsid-stabilizing compound. Complete inhibition required filling of most of the virion's binding pockets by this compound.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Capsid destabilization is required for antibody-mediated disruption of poliovirus

Delaet

Boeyé

1994

Journal of General Virology

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Update on the neutralisation of animal viruses

Dimmock

1995

Reviews in Medical Virology

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How a virus is neutralised depends upon several interacting factors. The most important of these, but by no means the only ones, are the interaction of a specific epitope, the properties of the cognate antibody, and the nature of the host cell. Variation in any one of these may change the mechanism by which neutralisation occurs and may obviate neutralisation altogether. The purpose of this article is to present an overview of neutralisation; for a more detailed discussion the reader should consult Dimmock' for references up to 1993 and more recent reviews2--' DEFINITIONSNeutralisation is the loss of infectivity which ensues when antibody molecule(s) bind to a virus particle, and usually occurs without the involvement of any other agency. As such this is an unusual activity of antibody paralleled only by the inhibition of toxins and enzymes. However, neutralisation by some antibodies may require or be assisted by, for example, complement or cells bearing receptors for the Fc portion of the antibody molecule. The term neutralisation is best used only in relation to in uifro systems as there is little information about how antibodies act in uiuo. The latter is defined here as protection. NEUTRALISATION EPITOPES Exposed epitopesMost epitopes are exposed and, in contrast to cryptic epitopes (see below), are available on the surface of a virion to bind antibody at all times. Most but not all viral neutralisation antigens are proteins. Figure I shows the relationship between epitope, antigenic site and antigen. An antigenic site is a collection of overlapping epitopes, but its definition is limited by the number of mAbs which are available for this purpose. In fact it is debatable whether or not discrete antigenic sites exist or' whether an antigen is a continuum of epitopes. However, the concept of an antigenic site highlights that there are regions of immunodominance to which the immune system responds by preference. Not all epitopes mediate Abbreviations used: CDR, complementary determining region. neutralisation. This is an important fact which emphasises that binding of antibody to an epitope is not in itself sufficient for neutralisation, and that neutralisation epitopes have the unique functional property which leads to loss of virus infectivity. This fact is further and very well demonstrated by some neutralising antibody-escape mutants which are still able to bind the selecting mAb (Table I; The epitope itself is unchanged, and it is likely that a mutation in another part of the molecule prevents neutralisation; this in turn implies that neutralisation via this epitope depends on the mAb being able to trigger an event within the neutralisation protein which occurs subsequent to binding. Cryptic epitopesAlthough postulated to occur some years ago, evidence for the existence of such epitopes has only recently been obtained. As their name indicates, cryptic epitopes are not normally available to bind antibody but are revealed by conformational changes in the virion which occur when it interacts with its environme...

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The influence of the milieu on the rate of neutralisation of herpes simplex virus 1

Bolt

Davies²,

Randall

et al. 1998

Arch. Virol.

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The rate of neutralisation of herpes simplex virus 1 was increased by up to more than five hundred-fold when the virus suspension and antiserum were each diluted to one hundred-fold in water instead of phosphate buffered saline. This phenomenon, which was observed for two human positive sera and a rabbit purified polyclonal antibody, may represent an unrecognised homeostatic mechanism where neutralising antibody is 'dilution-fast' under physiological conditions of transudation or pathological conditions of inflammation.

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Antigenic N to H conversion of poliovirus by a monoclonal antibody at low ionic strength

Cited by 8 publications

References 20 publications

Capsid destabilization is required for antibody-mediated disruption of poliovirus

Capsid destabilization is required for antibody-mediated disruption of poliovirus

Update on the neutralisation of animal viruses

The influence of the milieu on the rate of neutralisation of herpes simplex virus 1

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