Antigenic Phenotype of TdT-positive cells in human peripheral blood

Bradstock, Kenneth F.; Kerr, Arna; Bollum, F. J.

doi:10.1016/0008-8749(85)90223-0

Cited by 14 publications

(4 citation statements)

References 15 publications

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“…In a previous report, we described the incidence and membrane characteristics of TdT+ cells in normal childhood and adult peripheral blood (10). TdT+ cells were present at very low frequency (range: 0-001-0-019% of total nucleated cells) in these post-natal cells.…”

Section: Discussionmentioning

confidence: 93%

Section: Immunolluorescencementioning

confidence: 99%

“…Indirect immunofluorescence for surface membrane antigens was performed as described previously (10), using Moabs listed in Clusters of Differentiation (CD) according to the First and Seeond International Workshops on Human Leucocyte Differentiation antigens (11.12) ( Table 1). As negative controls, irrelevant Moabs (reacting specifically only with canine eells) of the same isotype were substituted.…”

Section: Immunolluorescencementioning

confidence: 99%

“…The frequeney of TdT+ cells was ealculated by eounting the total number of TdT"!" cells present on a c>tospin pellet, and relating this to the estimated total number of leucocytes on the slide, as described elsewhere (10). cord blood using Moabs to surface differentiation antigens are detailed in Table 2 Cord blood contained significantly lower numbers ofcells expressing E-rosette receptor (CD-2), CD-8 subset antigen, and the B cell antigen CD-24.…”

Section: Immunolluorescencementioning

confidence: 99%

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Phenotypic characterization of immature lymphoid cells in human umbilical cord blood

Bradstock

Kerr²,

Grimsley³

et al. 1988

Immunol Cell Biol

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SummaryThe antigenic phenotype of neonatal lymphoid cells isolated from umbilical cord blood was investigated using monoclonal antibodies and flow cytometry. Although the majority ofcells expressed mature T or B cell differentiation antigens, small subpopulations of phenotypically immature lymphocytes were detected. A small proportion (mean 2-8%) ofcells expressed the common acute lymphoblastic leukaemia antigen (CD-10), a significantly higher figure than that detected on adult peripheral blood lymphocytes. The cortical thymocyte antigen (CD-I) was detected on a very small subset of eord lymphoid eells, but was also present on adult lymphocytes at approximately the same frequency. The nuclear enzyme terminal deoxynucleotidyl transferase (TdT). a marker of early lymphoid differentiation, was detected by immunofluoreseenee on 0 031% of mononuclear cells in cytocentrifuge preparations, representing an approximate 10-fold increase in frequency over expression in childhood or adult blood. These circulating TdT+ cells were shown in double labelling experiments to predominantly express markers of B eell differentiation (CD-24, CD-10. MHC Class 2), although occasional cells co-expressing the T lineage marker CD-2 were also seen. These findings are consistent with the circulation of B cell precursors in neonatal blood. The nature ofthe CD-I + cells is unclear, although the absence of CD-1 ^ TdT"*" double labelled cells mitigates against the possible presenee of immature thymus-processed lymphocytes in these samples.

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Section: Discussionmentioning

confidence: 93%

Section: Immunolluorescencementioning

confidence: 99%

Section: Immunolluorescencementioning

confidence: 99%

Section: Immunolluorescencementioning

confidence: 99%

See 2 more Smart Citations

Phenotypic characterization of immature lymphoid cells in human umbilical cord blood

Bradstock

Kerr²,

Grimsley³

et al. 1988

Immunol Cell Biol

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Terminal deoxynucleotidyl transferase‐positive B cell precursors in fetal lymph nodes and extrahemopoietic tissues

et al. 1989

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Terminal deoxynucleotidyl transferase (TdT)-positive B cell precursors populate fetal lymph nodes (LN) of mid-term (16th-22th week) fetuses. These cells have a B cell phenotype (HLA-DR, CD45R, CD22, mu) and lack mature differentiation antigens (CD1c, CD20, L26). TdT+ cells are also present in the extranodal mesenteric mesenchyme, pancreas and LN primordia. Extranodal TdT+ cells are found among proliferating (i.e. Ki-67+) B cells and lack leukocyte function-associated antigen (LFA-1) and HLA-DQ; these latter molecules are acquired by nodal TdT+ elements. Adult LN, adult and fetal spleen contain less TdT+ cells than fetal LN. The fetal LN contains the full range of the B cell differentiation compartment, from the TdT+ precursor cell to the mature B lymphocyte.

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Minimal requirements for the diagnosis, classification, and evaluation of the treatment of childhood acute lymphoblastic leukemia (ALL) in the “BFM family” cooperative group

Vanderdoesvandenberg

Bartram

Basso

et al. 1992

Med. Pediatr. Oncol.

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Minimal requirements and their rationale for the diagnosis and the response to treatment in childhood acute lymphoblastic leukemia (ALL) were defined in the recently instituted "BFM-Family"-Group, in which the German, Austrian, Dutch, Italian, Belgian, French and Hungarian childhood leukemia study groups cooperate. ALL is defined as > or = 25% lymphoblasts in the bone marrow; for confirmation of the diagnosis and classification the criteria of the French-American-British (FAB) criteria are retained. For determination of the extent of the disease at diagnosis or relapse the criteria by the Rome Workshop [1986] are recommended: An obligatory panel of monoclonal antibodies for immunophenotyping was defined, as well as criteria for precursor B-ALL and T-ALL. Cytogenetic studies may support the diagnosis and subtyping, and are essential to identify certain patients with a high risk of treatment failure (f.i. t(9;22), t(4;11)). The role of molecular genetics for the diagnosis and the characterization of leukemia and the value of its clinical application needs further elucidation. Relapse was defined as recurrence of evident leukemia in the blood, bone marrow (> or = 25% lymphoblasts) or at any other site (to be confirmed by histological examination). Bone marrow involvement combined with extramedullary relapse was defined as > or = 5% lymphoblasts in the bone marrow.

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Antigenic Phenotype of TdT-positive cells in human peripheral blood

Cited by 14 publications

References 15 publications

Phenotypic characterization of immature lymphoid cells in human umbilical cord blood

Phenotypic characterization of immature lymphoid cells in human umbilical cord blood

Terminal deoxynucleotidyl transferase‐positive B cell precursors in fetal lymph nodes and extrahemopoietic tissues

Minimal requirements for the diagnosis, classification, and evaluation of the treatment of childhood acute lymphoblastic leukemia (ALL) in the “BFM family” cooperative group

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