Antigenic Relationships Among Type-3 Fimbriae of Enterobacteriaceae Revealed by Immunoelectronmicroscopy

Old, D. C.; Adegbola, Richard A.

doi:10.1099/00222615-20-1-113

Cited by 39 publications

(38 citation statements)

References 36 publications

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“…Haemagglutination, a property that reveals specific adhesinreceptor interactions, is associated with expression of P. mirabilis MR/P fimbriae (Old & Adegbola, 1985Adegbola et al, 1983). When haemagglutination was evaluated within our isolate collection, it was observed that every tested isolate was able to agglutinate fresh human erythrocytes.…”

Section: Haemagglutination Assaymentioning

confidence: 99%

Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice

2006

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Proteus mirabilis has been described as an aetiological agent in a wide range of infections, playing an important role in urinary tract infections (UTIs). In this study, a collection of P. mirabilis isolates obtained from clinical and non-clinical sources was analysed in order to determine a possible correlation between origin, virulence factors and in vivo infectivity. Isolates were characterized in vitro, assessing several virulence properties that had been previously associated with P. mirabilis uropathogenicity. Swarming motility, urease production, growth in urine, outer-membrane protein patterns, ability to grow in the presence of different iron sources, haemolysin and haemagglutinin production, and the presence and expression of diverse fimbrial genes, were analysed. In order to evaluate the infectivity of the different isolates, the experimental ascending UTI model in mice was used. Additionally, the Dienes test and the enterobacterial repetitive intergenic consensus (ERIC)-PCR assay were performed to assess the genetic diversity of the isolates. The results of the present study did not show any correlation between distribution of the diverse potential urovirulence factors and isolate source. No significant correlation was observed between infectivity and the origin of the isolates, since they all similarly colonized the urinary tract of the challenged mice. Finally, all isolates showed unique ERIC-PCR patterns, indicating that the isolates were genetically diverse. The results obtained in this study suggest that the source of P. mirabilis strains cannot be correlated with pathogenic attributes, and that the distribution of virulence factors between isolates of different origins may correspond to the opportunistic nature of the organism.

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Section: Haemagglutination Assaymentioning

confidence: 99%

Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice

2006

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“…Similar fimbrial appendages have since been found to be produced by many members of the Enterobacteriaceae (23,24). These long, thin fimbriae (4 to 5 nm by 0.5 to 2 ,um) are associated with hemagglutination of erythrocytes which have been treated with tannic acid, and hemagglutination occurs in the presence or absence of D-mannose.…”

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confidence: 94%

Molecular characterization of the type 3 (MR/K) fimbriae of Klebsiella pneumoniae

1988

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The expression of type 3 (MR/K) fimbriae by Klebsiella pneumoniae requires the production of at least four polypeptides with molecular masses of 20.5, 25, 34, and 78 kilodaltons. The genes encoding these polypeptides are located on a gene cluster 5,500 base pairs in length. The nucleotide sequence of the major fimbrial structural gene was determined, and its transcription was shown to initiate in vitro 157 base pairs upstream of the translational start site. The predicted amino acid sequence for the fimbrial subunit comprised 202 residues, including a signal peptide of 22 amino acids. Despite similarities in the organization of the gene cluster, the nucleotide and the amino acid sequence of the major fimbrial subunit revealed little agreement with other known fimbrial subunit sequences.

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“…dissolvens (Farmer et al, 1985; 1986hhave not yet been examined by us. These observations were not surprising in that similar antigenic heterogeneity has been demonstrated among the type-3, and other kinds of thin, fimbriae of species of Enterobacter Old and Adegbola, 1985).…”

Section: Discussionmentioning

confidence: 61%

“…Indeed, they seemed to be species-specific rather than, as described by Nowotarska and Mulczyk (1 977), genus-specific. The recent report of Old and Adegbola (1985) has also demonstrated that the type-3 fimbriae of the different species of Proteeae are species-specific.…”

Section: Discussionmentioning

confidence: 99%

“…Type-1 fimbriae are antigenic (Gillies and Duguid, 1958) and share serological relationships with non-haemagglutinating type-2 fimbriae of salmonellae (Old and Payne, 1971) but not with the type-3 fimbriae of Klebsiella (Nowotarska and Mulczyk, 1977;Adegbola, 1983;Korhonen et al, 1983;Old et al, 1985). Agglutination tests have been used to demonstrate sharing of type-1 fimbrial antigens among: (i) Escherichia, Shigella and, to a lesser extent, Klebsiella (Gillies and Duguid, 1958); (ii) Citrobacter and Salmonella (Duguid and Campbell, 1969).…”

Section: Introductionmentioning

confidence: 99%

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Antigenic relationships among type-1 fimbriae of Enterobacteriaceae revealed by immuno-electronmicroscopy

Adegbola¹,

OLD²

1987

Journal of Medical Microbiology

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Summary. Antigenic relationships among type-1 fimbriae of 40 strains of Enterobacteriaceae representing 19 species and seven genera were determined by immunoelectronmicroscopy. Ten distinct antigenic groups were distinguished. Intra-and inter-generic relationships were observed although some antigenic groups were species-specific only. Antigenic relationships among type-1 fimbriae are more complex than have been reported hitherto.

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Antigenic Relationships Among Type-3 Fimbriae of Enterobacteriaceae Revealed by Immunoelectronmicroscopy

Cited by 39 publications

References 36 publications

Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice

Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice

Molecular characterization of the type 3 (MR/K) fimbriae of Klebsiella pneumoniae

Antigenic relationships among type-1 fimbriae of Enterobacteriaceae revealed by immuno-electronmicroscopy

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