Antigenic relationships of coliform and related bacteria isolated from infants in the nurseries of two institutions

Routine Use of Liquid Urea Medium for Identifying Salmonella and Shigella Organisms

“…Most urease-producing paracolon organisms (and some strains of Proteus morganii) only produce the required alkalinity after incubation overnight. Except for Shigella alkalescens and Shigella equirulis (Stuart, Van Stratum, and Rustigian, 1945), salmonella and shigella organisms have not been reported to produce urease (Ferguson and Hook, 1943).…”

“…Literature The ability of the Proteus group to decompose urea with the formation of free ammonia and the production of alkalinity in the medium has been employed by various investigators in the identification of this group. Rustigian and Stuart (1941Stuart ( , 1943, and Stuart, Van Stratum, and Rustigian (1945) used a strongly buffered urea broth. In this medium Proteus group organisms produced enough ammonia to give a reaction more alkaline than pH 8.1 after 12-48 hours' incubation at 37°C.…”

Multitest media for rapid identification of Proteus species with notes on biochemical reactions of strains isolated from urine and pus

“…These included the urease test in the strongly buffered urea medium of Stuart, van Stratum, and Rustigian 1945); the phenylpyruvic acid (P.P.A.) test in phenylalanine medium of Edwards, Fife, and Ewing 1961); sugar fermentation reactions in 10%sugar medium containing Andrade's indicator and Durham's tube; indole production in 1 % peptone water tested by Ehrlich reagent; citrate utilization in Simmon's citrate medium; gelatin liquefaction in 2 ml.…”

Antigenic Studies of a Group of Paracolon Bacteria (Bethesda Group)

“…Rhodes (1942) found that several cultures isolated from one food-poisoning outbreak were identical. Stuart and Van Stratum (1945) found a high percentage of serologically identical coliform and paracolon strains in each of two institutional outbreaks of diarrhea in children. Barnes and Cherry (1946) noted that 28 strains isolated from an outbreak of gastroenteritis were serologically related, but the antigenic properties of the cultures were not studied intensively.…”

“…There is an increasing tendency to attribute etiological significance to these forms. The literature dealing with the role of paracolons in diarrhea has been reviewed by Neter and Clark (1944), Stuart and Van Stratum (1945), and Barnes and Cherry (1946).…”