Antigenic Relationships of the Species Shigella dispar.

Carpenter, Philip L.

doi:10.3181/00379727-53-14217p

Cited by 10 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, S. tiett is not the only Shigella type in which lactose fermentation varies. The antigenic characteristics of the Shigella (2-193) that was first found by Ewing, and which is identical with Neter's alkalescens III (Neter, 1944;De Assis, 1947a), are found in strains that do and and that do not form acid from lactose (Carpenter, 1943;Carpenter and Stuart, 1946). Under the predominance of cultural considerations, Ewing's 2-193 has been successively classified as a S. alkalescens, as S. dispar (Carpenter and Stuart, 1946), and as S. flexneri (Francis, 1946).…”

Section: Discussionmentioning

confidence: 86%

Shigella tieté

Weil¹,

Slafkovsky²

1948

J Bacteriol

View full text Add to dashboard Cite

De Assis in 1939 (a,b) described strain 648, which was related in cultural properties to Shigella alkalescens, although it, failed to ferment dulcitol. Antigenically it was entirely different from the ordinary S. alkalescens.\_ At that time this form was called alkalescens II, a designation that was adopted by Neter (1944). Approximately 20 strains of this type were isolated from cases of dysentery in Rio de Janeiro and Sao Paulo (De Assis et al., 1946; De Assis, 1947b). Two of the strains slowly utilized lactose (De Assis et al., 1946). In a recent publication, De Assis (1947b) designated these strains as Shigella tiet.1 Among the strains employed by Braun and Unat (1942a,b, 1943a,b) in their investigation of the inagglutinability of certain shigellae, a strain Clark was mentioned which, when received in this laboratory, proved to be culturally and serologically indistinguishable from S. tiete. The isolation of strains of this type at two points as distant as Brazil and Turkey suggested that S. tiete is more than a local Brazilian variant. For this reason, and because the confusion regarding this type reflects what may occur in the classification of shigellae in general, we feel justified in reporting our experiences with S. tiete. The strain Clark was obtained through the courtesy of Professor Braun both as "Ocl" and "Ol" variant. Dr. de Assis kindly sent us four of his strains including the lactose-fermenting cultures, "Gen" and "Cav." We are also indebted to Drs. Ewing, Francis, Neter, and Mollari for strains used in this work. S. tiete is a gram-negative rod that is nonmotile in fluid media and semisolid agar at 20 C and 37 C. It does not liquefy gelatin nor attack urea. It is anaerogenic, indole-positive, and it reduces trimethylamine oxide (Wood and Baird, 1943; Weil and Black, 1944). It forms acid rapidly from glucose, mannitol, maltose, and arabinose, and may or may not slowly utilize salicin. Dulcitol and rhamnose are not fermented. Lactose was not acidified by two of the strains of Dr. de Assis, but was slowly fermented by strains "Gen" and "Cav" as well as by Braun's strain Clark. On agar plates, the strains form both clear and opaque colonies, as observed in S. alkalescens (see figure 1 and references in Weil, 1947). All strains formed round smooth colonies. They grow evenly throughout fluid media, and suspensions are salt-stable. The clear variant is agglutinable. The opaque variant is inagglutinable when tested in the living state, but after boiling for 1 hour the bacteria are

show abstract

Section: Discussionmentioning

confidence: 86%

Shigella tieté

Weil¹,

Slafkovsky²

1948

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…On the other hand, the relatively weak agglutination by Flexner serums (1:320) and the reciprocal agglutination of Flexner Y in 2372 antiserum (1:320) when the homologous titers are 10,240 to 20,480 do not appear to be sufficient evidence to place this organism with the Flexner group. The closest serological relationship is to S. dispar, sero-type II (Carpenter, 1943(Carpenter, , 1945), yet it differs biochemically from S. dispar in the failure to ferment lactose.…”

Section: Discussionmentioning

confidence: 99%

“…When the relationships of these organisms to other members of the Shigella group were studied by reciprocal agglutination tests, certain reactions occurred of sufficient strength to deserve mention. Culture 2372 was most interesting in this respect since it was agglutinated by antiserums prepared against (1) S. paradysenteriae, Boyd type P143, to within one dilution of the homologous titer, (2) Shigella dis-par sero-type II (Carpenter, 1943(Carpenter, , 1945, to within one or two dilutions of the homologous titer, and (3) most Flexner types. Adsorption of type P143 antiserums by 2372 culture failed to reduce the homologous titer, and a major common antigen distinct from the specific P143 antigen must be postulated between type P143 and strain 2372.…”

Section: Serological Relationshipsmentioning

confidence: 99%

Antigenic Complex ofShigella paradysenteriae, Boyd Type P274

1946

View full text Add to dashboard Cite

“…The four suspensions were tested with 1:50, 1: 100, and 1: 200 dilutions of three Shigella sonnei antisera (two S and one R) and with six Shigella dispar antisera. The latter included types I, II, III, and IV (Carpenter, 1943(Carpenter, , 1944, a Shigella madampensis antiserum supplied by the Army Medical School, and an antiserum made with culture 10-888, isolated in Italy. Culture 10-888 bears some serological relationship to Carpenter's type II.…”

Section: Biochemical Studiesmentioning

confidence: 99%

AdditionalShigella paradysenteriaeSerotype

Ewing¹

1946

J Bacteriol

View full text Add to dashboard Cite

Antigenic Relationships of the Species Shigella dispar.

Cited by 10 publications

References 0 publications

Shigella tieté

Shigella tieté

Antigenic Complex ofShigella paradysenteriae, Boyd Type P274

AdditionalShigella paradysenteriaeSerotype

Contact Info

Product

Resources

About