Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7

Tan, Geok Hun; Yusoff, Khatijah; Seow, Heng Fong; Tan, Wen

doi:10.1002/jmv.20479

Cited by 34 publications

(26 citation statements)

References 26 publications

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“…VLPs formed by the capsid proteins of viruses such as HBV (25,27,32) and human papillomavirus (33) and bacteriophages (8,34) have been widely used for displaying foreign epitopes for vaccine development. The carriers enhance the antigenicity of the fused epitopes (35)(36)(37), which are often found to be inefficient in eliciting immune responses.…”

Section: Discussionmentioning

confidence: 99%

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Induction of Humoral and Cell-Mediated Immune Responses by Hepatitis B Virus Epitope Displayed on the Virus-Like Particles of Prawn Nodavirus

Yong

Yeap

Goh

et al. 2015

Appl Environ Microbiol

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Hepatitis B virus (HBV) is a deadly pathogen that has killed countless people worldwide. Saccharomyces cerevisiae-derived HBV vaccines based upon hepatitis B surface antigen (HBsAg) is highly effective. However, the emergence of vaccine escape mutants due to mutations on the HBsAg and polymerase genes has produced a continuous need for the development of new HBV vaccines. In this study, the "a" determinant within HBsAg was displayed on the recombinant capsid protein of Macrobrachium rosenbergii nodavirus (MrNV), which can be purified easily in a single step through immobilized metal affinity chromatography (IMAC). The purified protein self-assembled into virus-like particles (VLPs) when observed under a transmission electron microscope (TEM). Immunization of BALB/c mice with this chimeric protein induced specific antibodies against the "a" determinant. In addition, it induced significantly more natural killer and cytotoxic T cells, as well as an increase in interferon gamma (IFN-␥) secretion, which are vital for virus clearance. Collectively, these findings demonstrated that the MrNV capsid protein is a potential carrier for the HBV "a" determinant, which can be further extended to display other foreign epitopes. This paper is the first to report the application of MrNV VLPs as a novel platform to display foreign epitopes. Hepatitis B virus (HBV) is a partially double-stranded DNA virus that belongs to the family Hepadnaviridae. It is known to be the major cause of liver-associated diseases, including liver cirrhosis and hepatocellular carcinoma. Approximately 2 billion people worldwide have been infected by HBV, among which about 350 million chronically infected people serve as a reservoir for the virus (1, 2). To date, there is no effective treatment for HBV infection despite intensive studies on antiviral drugs. Thus, preventive measures, including immunization with HBV vaccine, remain necessary.HBV surface antigens (HBsAg) purified from the sera of infected patients have been used for immunization against HBV infection since 1981. The full-length HBsAg gene has three different start codons and a common stop codon and encodes HBsAg of different lengths (3). The longest is the large HBsAg (L-HBsAg), followed by the middle HBsAg (M-HBsAg), and the smallest is the small HBsAg (S-HBsAg). L-HBsAg is composed of 389 or 400 amino acids (aa), depending on the virus genotypes. It contains the pre-S1 region (108 or 119 aa), followed by the pre-S2 region (55 aa) and S-HBsAg (226 aa). The N-terminal end of M-HBsAg harbors the pre-S2 region at its S-HBsAg. The S-HBsAg contains 226 aa in the absence of the pre-S1 and pre-S2 regions.The HBsAg in serum self-assemble into noninfectious 22-nm spherical or filamentous particles, along with lipid components (4), which are capable of inducing neutralizing antibodies against HBV infection. Although highly effective, the use of plasma-derived HBsAg for vaccination is limited by the production cost, as well as the possibility of contamination by other infectious pathogens present in...

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Section: Discussionmentioning

confidence: 99%

“…HBV vaccines containing the "a" determinant often induce protective antibodies with high cross-reactivity against HBV of various subtypes (8,9). Despite the effectiveness of these yeastderived vaccines, HBV variants with mutations across the "a" determinant have been shown to escape the immune responses generated by the yeast-derived HBsAg (10)(11)(12)(13).…”

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confidence: 99%

Induction of Humoral and Cell-Mediated Immune Responses by Hepatitis B Virus Epitope Displayed on the Virus-Like Particles of Prawn Nodavirus

Yong

Yeap

Goh

et al. 2015

Appl Environ Microbiol

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“…Thus, it has been successfully applied to identify mimotopes used to diagnose malaria (22) (23) , toxoplasmosis (24) (25) , hepatitis A (26) , neurocysticercosis (27) , strongyloidiasis (28) , thyroid cancer (29) , Chagas' disease (30) , and bovine anaplasmosis (31) , and to develop vaccine candidates against cysticercosis (32) , herpes simplex virus infection (33) , cancer (34) , taeniasis (35) , hepatitis B (36) , trichinellosis (37) , Alzheimer's disease (38) , and bovine anaplasmosis (39) . Phage display technology has been also instrumental in the selection of therapeutic agents to treat various cancers such as glioblastoma, melanoma, leukemia, and prostate and thyroid cancers (40) (41) .…”

Section: Bacteriophages As Tools For the Development Of New Vaccinesmentioning

confidence: 99%

Theranostic applications of phage display to control leishmaniasis: selection of biomarkers for serodiagnostics, vaccination, and immunotherapy

Coelho

Chávez-Fumagalli

Costa

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Phage display is a high-throughput subtractive proteomic technology used for the generation and screening of large peptide and antibody libraries. It is based on the selection of phage-fused surface-exposed peptides that recognize specifi c ligands and demonstrate desired functionality for diagnostic and therapeutic purposes. Phage display has provided unmatched tools for controlling viral, bacterial, fungal, and parasitic infections, and allowed identifi cation of new therapeutic targets to treat cancer, metabolic diseases, and other chronic conditions. This review presents recent advancements in serodiagnostics and prevention of leishmaniasis -an important tropical parasitic disease-achieved using phage display for the identifi cation of novel antigens with improved sensitivity and specifi city. Our focus is on theranostics of visceral leishmaniasis with the aim to develop biomarker candidates exhibiting both diagnostic and therapeutic potential to fi ght this important, yet neglected, tropical disease.

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“…Among those, T7 phage has the ability to display small peptides in high-copy number and large peptides or proteins in low-or mid-copy number. This system is widely used (Tan et al, 2005;Herrmann et al, 2007;Shadidi et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

T7 phage displaying latent membrane protein 1 of Epstein-Barr virus elicits humoral and cellular immune responses in rats

Gao¹,

Liu²,

Huang³

et al. 2011

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Summary. -The latent membrane protein 1 (LMP1) encoded by Epstein-Barr virus (EBV) has become a potential target in EBV-associated tumor prevention and treatment due to its multiple biological effects. In this study, the recombinant T7 phage displaying full-length LMP1 protein was cloned and used as an immunogen to immunize rats. Results of flow cytometry, Western blot analysis, and ELISA confirmed that both humoral and cellular immune responses were elicited in the immunized rats. Our data suggested that T7 phage was an efficient antigen carrier. The recombinant T7-LMP1 phage reconstitutes the antigenic and immunogenic properties of LMP1 and can serve as a vaccine against EBV.

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Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7

Cited by 34 publications

References 26 publications

Induction of Humoral and Cell-Mediated Immune Responses by Hepatitis B Virus Epitope Displayed on the Virus-Like Particles of Prawn Nodavirus

Induction of Humoral and Cell-Mediated Immune Responses by Hepatitis B Virus Epitope Displayed on the Virus-Like Particles of Prawn Nodavirus

Theranostic applications of phage display to control leishmaniasis: selection of biomarkers for serodiagnostics, vaccination, and immunotherapy

T7 phage displaying latent membrane protein 1 of Epstein-Barr virus elicits humoral and cellular immune responses in rats

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