Antigenicity of Monocomponent Porcine Insulin in Rabbits

Kumar, Dileep; Sd, Mehtalia; Lv, Miller

doi:10.1055/s-0028-1093866

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…Kerp et al 2<) have shown that injection of proinsulin in rabbits does not lead to the formation of insulin antibodies but rather to low-titer proinsulinspecific antibodies. Kumar et al 21 have demonstrated that MC insulin is antigenic in rabbits. Schlichtkrull's experiments have been criticized in that some studies were carried out with the regular form of MC insulin in neutral solution.…”

Section: Discussionmentioning

confidence: 98%

Antigenicity of “Monocomponent” Pork Insulin in Diabetic Subjects

Yue¹,

Turtle²

1975

Diabetes

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"Single-peak," "single-component," and "monocomponent" insulins have been produced in an attempt to eliminate insulin antigenicity. Recently "single-peak insulin" has been shown to be antigenic. From animal experiments and preliminary human studies it has been claimed that monocomponent (MC) insulin is nonantigenic or only negligibly so. In this study the antigenicity of MC insulin was determined in two groups of diabetic patients. In group 1, seven patients treated with insulin for the first time were given MC insulin for seven to fifteen months. Four of the seven patients developed significant IgG insulin antibodies after four to ten months. In one patient the IgG insulin antibody concentration was high (8.51 mU./ml.). In two patients, IgG proinsulin-specific antibodies were detected. In group 2, fourteen patients with unstable diabetes, insulin allergy, or resistance were changed from conventional to MC insulin. Treatment with MC insulin did not decrease insulin requirement or improve diabetic control when assayed by the M factor. After seven to eleven months of therapy there was no significant fall in insulin antibodies except in two patients in whom corticosteroids had been administered simultaneously. These results differ significantly from those previously reported and could be interpreted as suggesting that insulin itself is antigenic. When the purity of the MC insulin was determined, significant contaminants could be demonstrated in all of ten separate batches of MC insulin. Gel chromatography, polyacrylamide gel electrophoresis, and proinsulin radioimmunoassay were used to identify the presence of nonconvertible insulin dimer, proinsulin, and monodesamido insulin in antigenically significant concentrations. The generation of IgG insulin antibodies in MC-insulin-treated patients cannot be interpreted as a true indication that insulin itself is antigenic. The problem of insulin antigenicity has not been resolved and will not be until a highly purified insulin is available. Unfortunately, the MC insulins do not meet these requirements.

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Section: Discussionmentioning

confidence: 98%

Antigenicity of “Monocomponent” Pork Insulin in Diabetic Subjects

Yue¹,

Turtle²

1975

Diabetes

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“…Kumar and Miller (1973) showed that a number of patients treated with commercial insulins did not develop proinsulin-specific antibodies, although they all formed insulin antibodies. The same authors were unsuccessful in demonstrating proinsulin-specific antibodies in commercial-insulin-treated rabbits (Kumar, Mehtalia and Miller 1974).…”

Section: Discussionmentioning

confidence: 99%

Insulin Antibodies and Immune Insulitis in Rabbits Immunized with Bovine or Porcine Insulin Components

Klöppel¹,

Freytag²

1975

Horm Metab Res

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The production and characteristics of anti-insulin, anti-a-component and anti-proinsulin antibodies in patients treated with monocomponent or conventional insulin

et al. 1979

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Highly purified pork monocomponent insulin produced less anti-insulin antibody than conventional insulins in diabetic patients. The smaller amount of anti-insulin antibody produced by MC insulin bound pork insulin more strongly than beef insulin in both displacement and direct binding studies of 125I-insulin. On the contrary, anti-insulin antibody which was produced by conventional insulins (beef insulin or mixture of pork and beef insulin) bound beef insulin more strongly. No significant anti-a-component and anti-proinsulin antibodies were detected in diabetics treated with highly purified monocomponent pork insulin about two years, compared to significant production of these antibodies in diabetics treated with conventional insulins. These results suggest that the species difference of the insulin molecule itself plays a significant role for the production of anti-insulin antibody, as the impurities do, in insulin-treated diabetic patients. The production of anti-insulin and anti-a-component antibodies decreased clearly after switching to highly purified monocomponent from conventional insulin. No effect of the switching on insulin requirement was found; however, better control of diabetes was accomplished in relation to the level of fasting blood sugar.

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Antigenicity of Monocomponent Porcine Insulin in Rabbits

Abstract: ceUular hypersensitivity in diabetes meUitus. Experimental induction of anti-pancreatic, cellular hypersensitivity and associated morphological B-cell changes in the rat. Acta allerg.

Cited by 4 publications

References 5 publications

Antigenicity of “Monocomponent” Pork Insulin in Diabetic Subjects

Antigenicity of “Monocomponent” Pork Insulin in Diabetic Subjects

Insulin Antibodies and Immune Insulitis in Rabbits Immunized with Bovine or Porcine Insulin Components

The production and characteristics of anti-insulin, anti-a-component and anti-proinsulin antibodies in patients treated with monocomponent or conventional insulin

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