Antigenotoxic effects of (-)-epigallocatechin-3-gallate (EGCG) and its relationship with the endogenous antioxidant system, 8-hydroxydeoxyguanosine adduct repair (8-OHdG), and apoptosis in mice exposed to chromium(VI)

Garcia‐Rodriguez, María del Carmen; Serrano-Reyes, Gabriela; Hernández-Cortés, Lourdes Montserrat; Altamirano-Lozano, Mario

doi:10.1080/15287394.2020.1867275

Cited by 15 publications

(6 citation statements)

References 59 publications

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“…Previously, Leonard et al [41] demonstrated in vitro that resveratrol scavenged •OH in JB6 cells exposed to Cr(VI). In a previous study in vivo with (-)-epigallocatechin-3-gallate (EGCG), García-Rodríguez et al [42] reported that 8-OHdG levels returned to control levels when EGCG and Cr(VI) were co-administered, contrasting with the findings of this study. They also found that co-administration of EGCG and Cr(VI) decreased the magnitude of MN increase compared to Cr(VI) alone [42], similar to the effect observed in this study with resveratrol.…”

Section: Discussioncontrasting

confidence: 99%

“…In a previous study in vivo with (-)-epigallocatechin-3-gallate (EGCG), García-Rodríguez et al [42] reported that 8-OHdG levels returned to control levels when EGCG and Cr(VI) were co-administered, contrasting with the findings of this study. They also found that co-administration of EGCG and Cr(VI) decreased the magnitude of MN increase compared to Cr(VI) alone [42], similar to the effect observed in this study with resveratrol. Hence, it is possible that when resveratrol was administered in combination with CrO 3 , the repair mechanisms were enhanced by this polyphenol, contributing to a reduction in MN levels.…”

Section: Discussioncontrasting

confidence: 99%

“…In the group treated with CrO 3 , there was a decrease in SOD activity accompanied by an elevation in GPx and CAT activities, which is consistent with previous findings. Both in vitro [49] and in vivo studies demonstrated the effects of oral [50] and ip [42] administration of Cr(VI) compounds on endogenous antioxidants, such as activities of SOD, CAT, GPx, heme oxygenase-1 (HO-1) and levels of GSH. Matés [51] proposed that SOD plays an important role as a first-line antioxidant defense enzyme by catalyzing the dismutation of O 2…”

Section: Discussionmentioning

confidence: 99%

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Protective Effect of Resveratrol against Hexavalent Chromium-Induced Genotoxic Damage in Hsd:ICR Male Mice

et al. 2022

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The aim of this study is to examine the ability of resveratrol to counteract hexavalent chromium [Cr(VI)]-induced genetic damage, as well as the possible pathways associated with this protection. Hsd:ICR male mice are divided into groups of the following five individuals each: (a) control 1, distilled water; (b) control 2, ethanol 30%; (c) resveratrol, 50 mg/kg by gavage; (d) CrO3, 20 mg/kg intraperitoneally; (e) resveratrol + CrO3, resveratrol administered 4 h prior to CrO3. The assessment is performed on peripheral blood. Micronuclei (MN) kinetics are measured from 0 to 72 h, while 8-hydroxydeoxyguanosine (8-OHdG) adduct repair levels, endogenous antioxidant system biomarkers, and apoptosis frequency were quantified after 48 h. Resveratrol reduces the frequency of Cr(VI)-induced MN and shows significant effects on the 8-OHdG adduct levels, suggesting that cell repair could be enhanced by this polyphenol. Concomitant administration of resveratrol and Cr(VI) results in a return of the activities of glutathione peroxidase and catalase to control levels, accompanied by modifications of superoxide dismutase activity and glutathione levels. Thus, antioxidant properties might play an important role in resveratrol-mediated inhibition of Cr(VI)-induced oxidant genotoxicity. The increase in apoptotic cells and the decrease in necrosis further confirmed that resveratrol effectively blocks the actions of Cr(VI).

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Protective Effect of Resveratrol against Hexavalent Chromium-Induced Genotoxic Damage in Hsd:ICR Male Mice

et al. 2022

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“…This protective phenomenon was noted with other metals, such as vanadium(V) (Rojas‐Lemus et al, 2014). CrO 3 exerted the most potent genotoxic effect among the tested compounds, resulting in formation of MN starting at 24 h and peaking at 48 h. These findings support and confirm previous observations regarding the genotoxicity of CrO 3 (García‐Rodríguez, López‐Santiago & Altamirano‐Lozano, 2021; García‐Rodríguez, Serrano‐Reyes et al, 2001; García‐Rodríguez, Santiago‐Moreno, et al, 2023). Conversely, K 2 CrO 4 exhibited the least amount of genotoxicity among the tested compounds.…”

Section: Discussionsupporting

confidence: 89%

“…The group treated with 20 mg/kg CrO 3 via i.p. injection was designated as the positive control in all protocols based upon historical data from our laboratory and previous studies, which demonstrated a significant increase in MN levels under the same working conditions (García‐Rodríguez et al, 2001, 2013, 2021; García‐Rodríguez, Santiago‐Moreno, et al, 2023). The evaluation criteria and experimental conditions were established in accordance with the Organisation for Economic Cooperation and Development (OECD) guidelines (Test No.…”

Section: Methodsmentioning

confidence: 99%

A comparative study on chromium‐induced micronuclei assessment in the peripheral blood of Hsd:ICR mice

García‐Rodríguez,

Hernández‐Cortés,

Montaño‐Rodríguez

et al. 2023

J of Applied Toxicology

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This study investigated the genotoxic effects of chromium (Cr) in Hsd:ICR mice, considering factors such as oxidative state, apoptosis, exposure pathway, duration, pregnancy, and transplacental exposure. Genotoxicity was assessed using the erythrocytes' micronucleus (MN) assay, while apoptosis was evaluated in nucleated blood cells. The results showed that Cr(III) (CrK(SO4)2 and CrCl3) did not induce any marked genotoxic damage. However, Cr(VI) (CrO3, K2Cr2O7, Na2Cr2O7, and K2CrO4) produced varying degrees of genotoxicity, with CrO3 being the most potent. MN frequencies increased following 24‐h exposure, with a greater effect in male mice. Administering 20 mg/kg of CrO3 via gavage did not lead to significant effects compared to intraperitoneal administration. Short‐term oral treatment with a daily dose of 8.5 mg/kg for 49 days elevated MN levels only on day 14 after treatment. Pregnant female mice exposed to CrO3 on day 15 of pregnancy exhibited reduced genotoxic effects compared to nonpregnant animals. However, significant increases in MN levels were found in their fetuses starting 48 h after exposure. In summary, data indicate the potential genotoxic effects of Cr, with Cr(VI) forms inducing higher genotoxicity than Cr(III). These findings indicate that gender, exposure route, and pregnancy status might influence genotoxic responses to Cr.

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Why traditional herbal medicine promotes wound healing: Research from immune response, wound microbiome to controlled delivery

Dong

Yin

et al. 2023

Advanced Drug Delivery Reviews

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Antigenotoxic effects of (-)-epigallocatechin-3-gallate (EGCG) and its relationship with the endogenous antioxidant system, 8-hydroxydeoxyguanosine adduct repair (8-OHdG), and apoptosis in mice exposed to chromium(VI)

Cited by 15 publications

References 59 publications

Protective Effect of Resveratrol against Hexavalent Chromium-Induced Genotoxic Damage in Hsd:ICR Male Mice

Protective Effect of Resveratrol against Hexavalent Chromium-Induced Genotoxic Damage in Hsd:ICR Male Mice

A comparative study on chromium‐induced micronuclei assessment in the peripheral blood of Hsd:ICR mice

Why traditional herbal medicine promotes wound healing: Research from immune response, wound microbiome to controlled delivery

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