Antigenotoxicity and antimutagenicity of lycopene in HepG2 cell line evaluated by the comet assay and micronucleus test

Scolastici, Clarissa; Lima, Rodrigo Otávio Alves de; Barbisan, Luı́s Fernando; Ferreira, Ana Lúcia dos Anjos; Ribeiro, Daniel Araki; Salvadori, Daisy Maria Fávero

doi:10.1016/j.tiv.2007.11.002

Cited by 67 publications

(43 citation statements)

References 28 publications

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“…The estimated lycopene content in 200 lg/ml of solvent extract is 18.56 lM. These results were consistent with Scolastici et al (2008), where lycopene at concentrations of 10-50 lM significantly reduced the genotoxic and mutagenic effects of H 2 O 2 and N-nitrosodiethylamine. Fig.…”

Section: Effects Of Extracts Against H 2 O 2 -Induced Cellular Dna Dasupporting

confidence: 88%

Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage

et al. 2010

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a b s t r a c tEffects of solvent and supercritical carbon dioxide (SC-CO 2 ) extraction on antioxidant and cytotoxic activities of lycopene-rich fractions of decanted pink guava by-product (decanter) were determined with lycopene-equivalent antioxidant capacity, b-carotene bleaching and MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide) assays. Extraction with SC-CO 2 gave a higher yield than solvent extraction (3.15 vs. 0.68 mg/100 g dried decanter, corresponding to 42.99 and 33.63 mg of lycopene).No cytotoxicity was found in Chang liver cells supplemented with either extracts (6.25-200 lg/ml). Solvent extract at 25 lg/ml (2.32 lM lycopene) and SC-CO 2 extract at 200 lg/ml (5.09 lM lycopene) had protective effect against hydrogen peroxide-induced cytotoxicity. However, only high concentrations of solvent extract (200 lg/ml; lycopene = 18.65 lM) or lycopene standard (10 lM) protected cells against DNA damage. Supercritical fluid extraction demonstrated a higher yield in lycopene-rich fraction from decanter. These fractions have the potential to be developed as a functional ingredient to prevent oxidative stress and other related diseases.

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Section: Effects Of Extracts Against H 2 O 2 -Induced Cellular Dna Dasupporting

confidence: 88%

Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage

et al. 2010

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“…The CBMN test provides simultaneous information on a variety of chromosomal damage endpoints that reflect chromosomal breakage and chromosome rearrangements (Scolastici et al, 2008). The combination of these methods is perfect to monitor the genotoxicity of QCT.…”

Section: Discussionmentioning

confidence: 99%

Investigation of quinocetone-induced mitochondrial damage and apoptosis in HepG2 cells and compared with its metabolites

Zhang

Wang

et al. 2015

Environmental Toxicology and Pharmacology

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“…It has been used extensively for in vitro studies and is particulary suitable for cytotoxicity models (Dai et al, 1993;Scolastici et al, 2008;Xu et al, 2003).…”

Section: Cytotoxic Potential Of Antioxidants From Tomatoes On Tumoralmentioning

confidence: 99%

Cytotoxic Potential of Antioxidants from Tomatoes on Tumoral Cells

Cenariu¹,

Pintea²,

Fischer‐Fodor³

et al. 2015

BUASVMCN-VM

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Tomatoes (Lycopersicon esculentum Mill.) are known to contain various antioxidants such as lycopene and other carotenoids, which were proven to have antineoplastic activity. The aim of the present study was to obtain and characterize a whole tomato extract in order to show the amount and type of antioxidants contained, as well as to assess the cytotoxic potential of such extracts on the HepG2 tumoral cell line. Tomato extracts were obtained using the light petroleum/ethyl acetate/methanol method and characterized by HPLC. HepG2 liver hepatocellular carcinoma cell line was grown in Eagle's Minimum Essential Medium (EMEM) supplemented with 10% FBS. As soon as subconfluency was reached cells were transferred into 96 well plates and treated with serial dilutions of the tomato extract. Cytotoxicity of the extracts was assessed using the MTT dye, in comparison with untreated cells and IC50 was established. The extraction method proved to be a very efficient one, yielding significant amounts of carotenoid pigments, including lycopene, β-carotene, γ-carotene and others, as shown by the HPLC analysis. The antioxidants contained by the extracts showed significant cytotoxicity on HepG2 liver hepatocellular carcinoma cell line, being able to visibly inhibit cellular development in vitro. The present study showed the cytotoxic influence that antioxidants extracted from tomatoes exert on the in vitro development of a hepatocellular carcinoma cell line, opening new perspectives for in vivo studies, involving patients with hepatic cancer.

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Antigenotoxicity and antimutagenicity of lycopene in HepG2 cell line evaluated by the comet assay and micronucleus test

Cited by 67 publications

References 28 publications

Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage

Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage

Investigation of quinocetone-induced mitochondrial damage and apoptosis in HepG2 cells and compared with its metabolites

Cytotoxic Potential of Antioxidants from Tomatoes on Tumoral Cells

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