Antigens of Helminths

Pery, P.; Luffau, G.

doi:10.1016/b978-0-12-635505-5.50008-1

Cited by 11 publications

(6 citation statements)

References 386 publications

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“…Very few functional antigens from helminths have been characterized or shown to be useful in vaccines (see reviews by Pery & Luffau, 1979;Clegg & Smith, 1978). For H. contortus, attention has been concentrated on the late larval stages.…”

Section: Discussionmentioning

confidence: 99%

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Vaccination of young lambs by means of a protein fraction extracted from adultHaemonchus contortus

1987

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SUMMARYLambs aged 48–150 days which had been injected with extracts enriched in a functional antigen (contortin) obtained from adult Haemonchus contortus developed specific circulating antibodies and were less susceptible to haemonchosis when challenged 1 month later with a single dose of 20000–25000 infective 3rd-stage larvae. Sera from 18 out of a total of 19 lambs injected with the extract contained precipitating antibodies to 2–5 components of the extract. None of these lambs died. The one extract-injected lamb which did not develop antibodies and 9 of the 13 lambs used as controls died of haemonchosis. The average weight of worms recovered was 1·45 g from the immune lambs and 5·72 g from the non-immune lambs.

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Section: Discussionmentioning

confidence: 99%

“…It is hoped, however, that there is sufficient to trigger a secondary response. Pery & Luffau (1979) pointed out that parasite components purified for vaccination purposes may be protective without being major immunological targets in natural infections.…”

Section: Discussionmentioning

confidence: 99%

Vaccination of young lambs by means of a protein fraction extracted from adultHaemonchus contortus

1987

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“…Worm homogenates and excretory/secretory/metabolic products protect recipients against homologous challenge infections in laboratory trials (Clegg and Smith, 1978;Murray et al, 1979;Pery and Luffau, 1979;Mitchell et al, 1984;Monroy, Brindley and Dobson, 1985). However, they are complex and even the comparatively simple excretory/secretory products are a heterogenous mixture of components (Day et al, 1979).…”

Section: Introductionmentioning

confidence: 99%

PROTECTIVE ANTIGENS IN FRACTIONS OF ADULT NEMATOSPIROIDES DUBIUS

Monroy

Dobson

1986

Aust J Exp Biol Med

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Summary Adult Nematospiroides dubius crude soluble homogenate consistently produced five fractions (Fl‐5) after filtration through Sephadex G‐200 with molecular weights of >300,000, 240,000, 83,000, 31,000 and < 10,000. The high molecular weight Fl and F2 reacted strongly with serum from previously infected mice in ELISA. Protective immunity was associated with Fl N. dubius immunogens (molecular weight > 300,000). Immunization with smaller molecular weight fractions also reduced worm recoveries following challenge infections but to a lesser extent.

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“…Many attempts have been made to isolate and characterize the major allergens from the closely re lated helminths Ascaris lumbricoides and Ascaris suum but in the majority of studies, the definition of allergenicity is based on data obtained from animal models rather than on those derived from human studies [reviewed in 8,13,17]. Allergenicity in these models is primarily associated with a few low molec ular weight allergens (range 15,000-45,000 daltons) of restricted isoelectric point (pH 5-6), whereas when assayed against human sera [15,23] allergens from A. suum demonstrate a wider range of both appar ent molecular weight (14,000-> 360,000) and pi (pH [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

An Immunochemical Investigation of the Allergens from <i>Ascaris suum </i>Perienteric Fluid

McWilliam¹,

Stewart²,

Turner³

1987

Int Arch Allergy Immunol

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An extract of Ascaris suum perienteric fluid was fractionated on Sephadex G-200 and the resulting high molecular weight (approx. 600,000 daltons) and low molecular weight (approx. 16,000 daltons) fractions examined for allergenicity using human sera. Radioallergosorbent (RAST) and RAST-inhibition assays showed that the high molecular weight fraction was 15- to 40-fold more potent than the low molecular weight fraction. Crossed radio-immunoelectrophoretic analysis showed that the high molecular weight fraction contained 10 allergens in contrast to the 6 comprising the low molecular weight fraction. Polyacrylamide gel electrophoretic studies in combination with RAST indicated that two high molecular weight glycoprotein-staining components may be major allergens. A. suum cross-reacted allergenically with Toxocara canis and various life cycle stages of Nippostrongylus brasilliensis but not with Strongyloides ratti or Schistosoma mattheei. The cross-reacting allergens were predominantly of high molecular weight and the molecular weight distribution pattern obtained for A. suum, T. canis and N. brasilliensis paralleled that obtained for the distribution of the carrier of the hapten phosphorylcholine (Pc). However, it was shown that the hapten per se was not allergenic. In contrast, analysis of the allergens from N. brasilliensis using sera from rats infected with the same parasite showed that the homologous allergens were predominantly of low molecular weight (approx. 20,000 daltons) and did not contain Pc.

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Antigens of Helminths

Cited by 11 publications

References 386 publications

Vaccination of young lambs by means of a protein fraction extracted from adultHaemonchus contortus

Vaccination of young lambs by means of a protein fraction extracted from adultHaemonchus contortus

PROTECTIVE ANTIGENS IN FRACTIONS OF ADULT NEMATOSPIROIDES DUBIUS

An Immunochemical Investigation of the Allergens from <i>Ascaris suum </i>Perienteric Fluid

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