Antihemolytic Activities of Green Tea, Safflower, and Mulberry Extracts during<i>Plasmodium berghei</i>Infection in Mice

Audomkasok, Suthin; Singpha, Waraporn; Chachiyo, Sukanya; Somsak, Voravuth

doi:10.1155/2014/203154

Cited by 17 publications

(9 citation statements)

References 20 publications

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“…Different plants showed variable inhibition levels depending on free radical sources. Polyphenolic compounds and flavonoids found in extracts (Table 1) play a crucial role in the protection of RBC from oxidative damages, preventing hemolysis through scavenging action of free radicals, ROS and RNS (Khalili, Mohammad, & Yaghoub, 2014;Audomkasok, Warapom, Sukanya & Voravuth, 2014).…”

Section: Discussionmentioning

confidence: 99%

Anti-hemolytic, Anti-lipid Peroxidation and Antioxidant Properties of Three Plants Locally Used to Treat Metabolic Disorders: Allium sativum, Persea americana and Citrus sinensis

Azantsa¹,

Mbong²,

Takuissu³

et al. 2019

JFR

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Citrus sinensis, Persea americana and Allium sativum are good sources of large number of various bioactive substances including antioxidants. Antioxidants play an essential role in the prevention and treatment of type 2 diabetes and other metabolic diseases. This study was conducted to evaluate and compare the anti-lipid peroxidation, anti-hemolytic and antioxidant capacity of aqueous extracts of the three plants in vitro. Polyphenolic and flavonoids content of aqueous extracts (EA) of plants were determined. The anti-lipid peroxidation was evaluated on homogenates of rat liver. Anti-haemolytic effects were assessed using erythrocyte system model induced free radicals from human red blood cells. Total antioxidant capacity and antiradical or scavenging capacity were evaluated on biological free radical systems (OH, NO . ) and synthetic radicals (DPPH and ABTS + ). Results showed that, different extracts possess variable amounts of polyphenolic compounds. They exhibited significant anti-hemolytic and anti-lipid peroxidation activities. Aqueous extracts of Citrus sinensis are powerful scavenger of OH . (IC 50 =1.05x10 -3 mg/ml ) and NO . (IC 50 = 1.29x10 -3 mg/ml) free radicals while Persea americana seed extract possesses the highest anti-lipid peroxidation capacity (59.72%) and the best scavenging capacity against DPPH and ABTS. Red blood cells were highly protected (at 97.87%, p<0.05) by Allium sativum extract at 1mg/mL and Citrus sinensis (87.7%, p<0.05). Therefore, anti-hemolytic, anti-lipid peroxidation effects of the aqueous extracts of the three plants can justify their use and efficacy in alternative treatment of metabolic diseases.

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Section: Discussionmentioning

confidence: 99%

Anti-hemolytic, Anti-lipid Peroxidation and Antioxidant Properties of Three Plants Locally Used to Treat Metabolic Disorders: Allium sativum, Persea americana and Citrus sinensis

Azantsa¹,

Mbong²,

Takuissu³

et al. 2019

JFR

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“…The oxidants such as t -butyl hydroperoxide and artemisinin-catalyzed epoxide can damage malaria parasites and PRBC [ 40 , 41 ]. Potent antioxidant GTE significantly decreased plasma levels of blood urea nitrogen and creatinine significantly in P. berghei ANKA-infected mice and consequently reversed their kidney function caused by oxidative stress condition [ 23 , 42 ]. Controversially, EGCG and M30 (an iron chelator) did not improve survival of P. berghei ANKA-infected mice [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effect of novel iron chelator, 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) and green tea extract on growth of Plasmodium falciparum

Thipubon

Uthaipibull

Kamchonwongpaisan

et al. 2015

Malar J

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BackgroundIron is an essential micronutrient required by all living organisms including malaria parasites (Plasmodium spp.) for many biochemical reactions, especially growth and multiplication processes. Therefore, malaria parasite needs to take up the iron from outside or/and inside the parasitized red blood cells (PRBC). Iron chelators are widely used for the treatment of thalassaemia-related iron overload and also inhibit parasite growth at levels that are non-toxic to mammalian cells.MethodsInhibitory effect of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) and green tea extract (GTE) on the growth of malaria parasite Plasmodium falciparum was compared with standard chelators including desferrioxamine (DFO), deferiprone (DFP) and deferasirox (DFX). A flow cytometric technique was used to enumerate PRBC stained with SYBR Green I fluorescent dye. The labile iron pool (LIP) was assayed using the calcein-acetoxymethyl fluorescent method.ResultsThe IC50 values of DFO, GTE, CM1, DFX and DFP against P. falciparum were 14.09, 21.11, 35.14, 44.71 and 58.25 µM, respectively. Importantly, CM1 was more effective in reducing LIP levels in the P. falciparum culture than DFP (p < 0.05).ConclusionsCM1 and GTE exhibit anti-malarial activity. They could interfere with uptake of exogenous iron or deplete the intracellular labile iron pool in malaria parasites, leading to inhibition of their growth.

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“…Also attributed to ergosterol such as ergosteryl ester, gallic, homogentisic, protocatechuic, phydroxybenzoic, p-coumaric acids, alkaloids, steroids, terpenoids [25] and selenium which protect cells from damage that might lead to chronic diseases and help to strengthen the immune system, as well [26]. Tumouricidal and cytotoxici-ty effects; antimetastic activity; Immunoprotective effects during radiation and chemotherapy [36].…”

Section: Resultsmentioning

confidence: 99%

Immunohistochemical Detection of Caspase 8 Expression and Apoptotic index Activities of Tephrosia purpurea Crude Extract in Balb/C Mice Inoculated with HepG2 Cells

Hade¹,

Tareq²,

Kadhim³

et al. 2019

djps

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The aim of this study was to identify immunomodulatory, apoptosis inducing and anticancer activities of aqueous and alcoholic extracts of T. purpurea, regarding the size of tumor mass and immunohistochemical detection of caspase 8 expressions and apoptotic index in mice inoculated with HepG2, mouse hepatocellular carcinoma as therapeutic experimental system for human. Forty-eight Balb / C albino mice were injected at legs with HepG2 cells. Tumor size was measured twice a week, note that the measurement time period is one month. Administration of aqueous extract (0.3,0.6,1.2) mg / kg and alcoholic extract of in (0.25,0.5,1) mg/kg to HepG2 tumor-bearing mice showed a significant difference (P≤0.01) in tumor size compared with control group. Inhibitory activity of aqueous and alcoholic extracts was dose and duration dependent. The effective doses in reduction of tumor size were 1.2 mg / kg for aqueous and 1mg/kg for alcoholic extract in which HepG2 tumor mass reduced in size for up to 87.9 %. HepG2 bearing mice treated with T. purpurea aqueous and alcoholic extracts showed significant increase (P ≤ 0.05) in apoptotic index compared with untreated control group. Extracts of T. purpurea were highly efficient for tumor growth inhibition, causing reduction in the tumor size, and increasing the expression of caspase 8 gene product in tumor tissue. Also,

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Antihemolytic Activities of Green Tea, Safflower, and Mulberry Extracts duringPlasmodium bergheiInfection in Mice

Cited by 17 publications

References 20 publications

Anti-hemolytic, Anti-lipid Peroxidation and Antioxidant Properties of Three Plants Locally Used to Treat Metabolic Disorders: Allium sativum, Persea americana and Citrus sinensis

Anti-hemolytic, Anti-lipid Peroxidation and Antioxidant Properties of Three Plants Locally Used to Treat Metabolic Disorders: Allium sativum, Persea americana and Citrus sinensis

Inhibitory effect of novel iron chelator, 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) and green tea extract on growth of Plasmodium falciparum

Immunohistochemical Detection of Caspase 8 Expression and Apoptotic index Activities of Tephrosia purpurea Crude Extract in Balb/C Mice Inoculated with HepG2 Cells

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