Antihemolytic and antioxidant activities of Allium paradoxum

Abstract

Antioxidant activity of the aerial part and bulbs of Allium paradoxum was investigated by eight in vitro assay systems. Extracts showed good antioxidant activity. IC 50 for 1,1-diphenyl-2-picryl hydrazyl radical-scavenging activity was 890.9±43.2 and 984.9±33.5 µg/ml for the aerial part and bulbs, respectively. The aerial parts have better reducing power than bulb extracts but not comparable with Vitamin C (P<0.001). Extracts showed weak Fe 2+ chelating ability, the IC 50 being 959±47 and 530±24 µg/ml for bulbs and aerial parts, respectively. Both tested extracts exhibited good hydrogen peroxide scavenging in a concentration dependent manner. They exhibited good antioxidant activity against the hemoglobin-induced linoleic acid system that was comparable with vitamin C (P<0.01). They showed good activity against cumene hydro peroxide induced hemolysis in RBCs. In addition, they possessed antihemolytic activity. The extract from aerial parts had significantly higher total phenol and flavonoid content than did bulbs. Amounts of eight elements (Cu, Mn, Zn, Fe, Ni, Pb, Cd and Cr) were also determined in the bulb and aerial part using atomic absorption spectroscopy. They contained higher Fe and Mn contents than other elements. © Versita Sp. z o.o.

Antihaemolytic activity of thirty herbal extracts in mouse red blood cells

“…RBCs were isolated and stored according to the method described by Ebrahimzadeh et al (7). Briefly, blood samples were centrifuged (1500 g, 10 min), and RBCs separated from plasma and buffy coat and washed three times by centrifugation (1500 g, 5 min) in 10 volumes of 10 mmol L -1 phosphate buffered saline (pH 7.4; PBS).…”

“…Peroxides such as H 2 O 2 and cumene hydroperoxide (7), protein denaturation, and heat shock-induced damage have all been extensively studied in biological membranes (7,20,21).…”

“…Several herbal secondary metabolites such as flavonoids have been found to protect cells from oxidative damage. These compounds have been evidenced to stabilise RBC membrane by scavenging free radicals and reducing lipid peroxidation (3,7).…”

“…Herbs are a rich source of flavonoids, phenolic acids, and alkaloids, some of which act as antioxidants (7)(8)(9)(10). Nabavi et al (9) studied the antioxidant and antihaemolytic activities of Ferula foetida in RBCs and a few other interesting studies have recently been reported, indicating the protective effects of plant extracts against oxidative damage in intact RBC membranes (7,9,10).…”

Biological activities of ethyl acetate extract of different parts ofHyssopus angustifolius

“…Results were expressed as gallic acid equivalents. Total flavonoid content was measured by employing the method of Ebrahimzadeh et al, (2010). 0.5 mL of sample at 1.6 mg mL −1 was separately mixed with 1.5 mL of methanol, aluminum chloride (10%, 0.1 mL), potassium acetate (1 M, 0.1 mL) and distilled water (2.8 mL) and incubated for 30 min at room temperature.…”