Antihepatotoxic benefits of <i>Poria cocos</i> polysaccharides on acetaminophen‐lesioned livers in vivo and in vitro

Wu, Ka; Guo, Chao; Yang, Bin; Wu, Xinmou; Wang, Weimin

doi:10.1002/jcb.28022

Cited by 45 publications

(24 citation statements)

References 24 publications

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“…And then, the sections were developed with diaminobenzidine for immunohistochemistry stains, and DAPI for immunofluorescence stains. Subsequently, the immunohistochemical sections were photographed by using an optical microscope (Olympus, Japan) and the immunofluorescence sections were imaged through an inverted fluorescent microscope (Olympus, Japan) …”

Section: Methodsmentioning

confidence: 99%

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Network analysis, and human and animal studies disclose the anticystitis glandularis effects of vitamin C

Guo

Liang

et al. 2019

BioFactors

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Background Our present study aimed to unravel the therapeutic biotargets of vitamin C (VC) against cystitis glandularis (CG), and to elucidate the molecular mechanisms for VC treating CG. Methods Network pharmacology was used to predict therapeutic targets of VC against CG, and to identify molecular mechanisms. In addition, further human and animal studies were designed to validate the bioinformatic findings through biochemical tests, computerized tomography scans, and immunostaining assays. Results In bioinformatic analyses, pathogenic targets of CG and putative targets of VC were identified, respectively. An interaction network between biological target and functional protein was produced before screening and collecting the key therapeutic targets of VC against CG, biological processes, and signaling pathways. In addition, ingenuity pathway analysis with cloud platform indicated that anti‐CG mechanisms of VC were achieved through modulating a cluster of molecular pathways, such as tumor necrosis factor (TNF) pathway. Meanwhile, 18 core targets of VC against CG were identified, and the most important TNF, interleukin‐6 (IL6), and Jun biotargets were obtained, respectively. In further validation in human study, cellular TNF‐α, IL6, and c‐Jun expressions in patient's CG samples were elevated significantly, accompanied with detectable urinary tract infection. Beneficially, VC‐dosed CG mice resulted in downregulated expressions of endogenous TNF‐α, IL6, and c‐Jun in blood and bladder samples. Conclusion Collectively, these bioinformatic findings and experimentative data uncover the therapeutic targets and biological mechanisms of VC for treating CG, in which the key biomarkers of TNF‐α, IL6, and c‐Jun may be the potential molecules for treating CG in clinical application.

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Section: Methodsmentioning

confidence: 99%

“…Subsequently, the immunohistochemical sections were photographed by using an optical microscope (Olympus, Japan) and the immunofluorescence sections were imaged through an inverted fluorescent microscope (Olympus, Japan). 18,19…”

Section: Immunostaining Protocolsmentioning

confidence: 99%

Network analysis, and human and animal studies disclose the anticystitis glandularis effects of vitamin C

Guo

Liang

et al. 2019

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“…The contents of matrix metalloproteinase 2 (MMP2) in human, cell line, and nude mice samples were examined by using the ELISA kits (CUSABIO, China). All experiments were independently conducted at least three times …”

Section: Methodsmentioning

confidence: 99%

“…All experiments were independently conducted at least three times. 19,20 2.6 | Quantitative RT-PCR procedures As described previously, 21,22 the RNA samples were, respectively, isolated from human, cell line, and nude mice by using the Trizol kits (Thermo). The maximal purity of RNAs was measured by using a NanoDrop ultra-micro spectrophotometer (Thermo).…”

Section: Mtt and Elisa Testsmentioning

confidence: 99%

Antimetastatic effects of calycosin on osteosarcoma and the underlying mechanism

Qiu

Qin

et al. 2019

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Osteosarcoma (OS) refers to a malignant tumor with potential invasiveness and metastasis; however, the current chemotherapy of OS is lacking. Thus, the alternative drug for treating OS is urgent to explore. Calycosin (CC) is evidenced in our previous study to play the anti‐OS benefits for suppressing cancer cell proliferation. Consequently, further investigation of CC‐medicated anti‐invasive and metastatic effects against OS is needed. In the current study, the clinical samples of OS patients were collected for biological and staining assays, such as enzyme‐linked immunosorbent assay and polymerase chain reaction. Meanwhile, the cell line and tumor‐bearing nude mice were employed in assessing antimetastatic effects of CC against OS through biochemical tests and immunoassays. As a result, the OS patients exhibited upregulated neoplastic expressions of matrix metalloproteinase 2 (MMP2) and proliferating cell nuclear antigen (PCNA), cellular mRNAs and proteins of inhibitor of nuclear factor kappa‐B alpha (IκBα), and epithelial cell transforming sequence 2 (ECT2). In cell‐line study, CC‐treated human OS cells exhibited induced cell apoptosis, reduced cell proliferation, and cellular MMP2 and PCNA concentration, inhibited cell migration, lowered expressions of IκBα ECT2 mRNAs, and proteins. In tumor‐bearing nude mice study, CC‐treated mice resulted in the dose‐dependent reductions of tumor weights and intracellular MMP2 contents. As shown in further assays, neoplastic expressions of interleukin 6 protein, IκBα, ECT2 mRNAs, and proteins were downregulated dose‐dependently in CC‐treated tumor‐bearing mice. In conclusion, these investigative findings suggest that CC may play the potential anti‐invasive benefits against OS through suppressing metastasis‐associated IκBα/ECT2 molecular pathway.

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“…According to the existing research literature, it can be clearly seen that the main active constituents of Poria cocos are concentrated on triterpenes and polysaccharides ( Jia et al, 2016 ). Some of these constituents possessed a series of biological activities including anti-tumor ( Sun and Xia, 2018 ), hepatoprotective ( Wu et al, 2019 ), anti-inflammatory ( Lee et al, 2017 ), anti-oxidant ( Wang et al, 2016 ), anti-bacterial ( Wang et al, 2019a ), immunomodulation( Pu et al, 2019 ), etc. Therefore, these complex chemical compounds and pharmacological effects of Poria cocos attracted researcher’s considerable attention, meanwhile, they also brought huge challenges for research.…”

Section: Introductionmentioning

confidence: 99%

Phytochemistry and Pharmacological Activities of Wolfiporia cocos (F.A. Wolf) Ryvarden & Gilb

et al. 2020

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Poria cocos is the dried sclerotium of Wolfiporia cocos (F.A. Wolf) Ryvarden & Gilb., which was the current accepted name and was formerly known as Macrohyporia cocos (Schwein.) I. Johans. & Ryvarden, Pachyma cocos (Schwein.) Fr., Poria cocos F.A. Wolf and Sclerotium cocos Schwein. It is one of the most important crude drugs in traditional Chinese medicine, with a wide range of applications in ameliorating phlegm and edema, relieving nephrosis and chronic gastritis and improving uneasiness of minds. Its extensive pharmacological effects have attracted considerable attention in recent years. However, there is no systematic review focusing on the chemical compounds and pharmacological activities of Poria cocos. Therefore, this review aimed to provide the latest information on the chemical compounds and pharmacological effects of Poria cocos, exploring the therapeutic potential of these compounds. We obtained the information of Poria cocos from electronic databases such as SCI finder, PubMed, Web of Science, CNKI, WanFang DATA and Google Scholar. Up to now, two main active ingredients, triterpenes and polysaccharides of Poria cocos, have been identified from Poria cocos. It has been reported that they have pharmacological effects on anti-tumor, anti-bacterial, anti-oxidant, anti-inflammatory, immunomodulation, and liver and kidney protection. The review summarizes the phytochemistry and pharmacological properties of Poria cocos, which suggest that researchers should focus on the development of new drugs about Poria cocos to make them exert greater therapeutic potential.

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Antihepatotoxic benefits of Poria cocos polysaccharides on acetaminophen‐lesioned livers in vivo and in vitro

Cited by 45 publications

References 24 publications

Network analysis, and human and animal studies disclose the anticystitis glandularis effects of vitamin C

Network analysis, and human and animal studies disclose the anticystitis glandularis effects of vitamin C

Antimetastatic effects of calycosin on osteosarcoma and the underlying mechanism

Phytochemistry and Pharmacological Activities of Wolfiporia cocos (F.A. Wolf) Ryvarden & Gilb

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