Antiherpesvirus Activity of the Combination of 5-iodo-2′-deoxycytidine with methotrexate: An<i>in vitro</i>and<i>in vivo</i>Study

Cremonesi, Caterina; Scarpini, Cinzia G.; Bianchi, Rossella; Radaelli, A.; Gimelli, Margherita; Morghen, Carlo De Giuli

doi:10.1177/095632029400500501

Cited by 2 publications

(3 citation statements)

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“…Our observations in evaluating the drug antiviral efficacy (e.g. difference between the active substance and the nearby vehicle control) suggest that the results appear to be independent from the position on the rostral-caudal axis, in agreement to what reported also by Cremonesi et al [4].…”

Section: Discussionsupporting

confidence: 93%

“…Recurrent herpes simplex labialis (HSV-1 infection) represents a disease still difficult to treat, despite 35 years of research dedicated to identify effective antiviral compounds. Although differences between the disease in humans and that obtained experimentally in animals suggest caution in predicting an effective clinical response, mouse [1,2] and especially guinea pig [3,4] are considered suitable experimental models for in vivo trials. The intradermal inoculation of the dorsal guinea pig cutaneous surface was shown to be the most useful system for studying the activity of antiviral compounds in topical formulations [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…Although differences between the disease in humans and that obtained experimentally in animals suggest caution in predicting an effective clinical response, mouse [1,2] and especially guinea pig [3,4] are considered suitable experimental models for in vivo trials. The intradermal inoculation of the dorsal guinea pig cutaneous surface was shown to be the most useful system for studying the activity of antiviral compounds in topical formulations [3,4]. In this species it is possible to obtain, after intradermal inoculation with HSV-1 at a high titer, cutaneous lesions very similar to, and developing in the same fashion as those observed in spontaneous human disease [5].…”

Section: Introductionmentioning

confidence: 99%

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Value of the Dorsal Cutaneous Guinea Pig Model in Selecting Topical Antiviral Formulations for the Treatment of Recurrent Herpes simplex Type 1 Disease

Poli

Dall’Ara

Binda

et al. 2011

Arzneimittelforschung

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Recurrent herpes simplex labialis represents a disease still difficult to treat, despite the availability of many established antiviral drugs used in clinical research since 30 years ago. Although differences between the human disease and that obtained in experimental animal suggest caution in predicting an effective clinical response from the experimental results, some of the animal models seem to be useful in optimising the topical formulation of single antiviral drugs. In the present work the dorsal cutaneous guinea pig model was used to compare 5 different topical antiviral formulations with clinical promise (active molecule: 5% w/w micronized aciclovir, CAS 59277-89-3), using both roll-on and lipstick application systems. The aim being to evaluate which vehicle (water, oil, low melting and high melting fatty base) and application system (roll-on, lipstick) enhances the skin penetration and the antiviral activity of the drug, after an experimental intradermal infection with Herpes simplex virus type 1 (HSV-1). As reference, a commercial formulation (5% aciclovir ointment) was used. The cumulative results of this study showed that the formulation A, containing 5% aciclovir in an aqueous base in a roll-on application system, has the better antiviral efficacy in reducing the severity of cutaneous lesions and the viral titer; among the lipsticks preparations, the formulation D, containing 5% aciclovir in a low melting fatty base, demonstrates a very strong antiviral activity, though slightly less than formulation A. This experimental work confirms the validity of the dorsal cutaneous guinea pig model as a rapid and efficient method to compare the antiviral efficacy of new formulations, with clinical promise, to optimise the topical formulation of the active antiviral drugs.

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Section: Discussionsupporting

confidence: 93%