Antihistamines and production of granulocyte-macrophage colony-stimulating factor and interleukin-8 by human bronchial epithelial cells in vitro: Evaluation of the effects of loratadine and cetirizine

Amsellem, Carole; Gormand, F.; Hosni, R.; Aloui, R.; Guibert, Benoît; Czarlewski, W.; Mélac, M.; Lagarde, Michel; Perrin‐Fayolle, M; Pachéco, Yves

doi:10.1016/0306-3623(95)02009-8

Cited by 7 publications

(4 citation statements)

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“…3 Noah et al 27 suggested that a correlation exists between calcium influx and IL-6 secretion in a bronchial epithelial cell line in response to stimulation with histamine (100 µmol/L). However, using the antihistamines loratadine and cetirizine hydrochloride, Ansellem et al 28 failed to demonstrate an inhibitory effect on cytokine secretion from bronchial epithelial cells. These investigators therefore suggested that histamine does not play a role in cytokine production.…”

Section: Commentmentioning

confidence: 99%

Inhibition of Histamine-Induced Human Conjunctival Epithelial Cell Responses by Ocular Allergy Drugs

Yanni

Weimer²,

Sharif³

et al. 1999

Arch Ophthalmol

100

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To evaluate the effects of topical ocular drugs with histamine H 1 -antagonist activity on histaminestimulated phosphatidylinositol turnover and interleukin (IL) 6 and IL-8 secretion from human conjunctival epithelial cells.Methods: Primary human conjunctival epithelial cell cultures were stimulated with histamine in the presence or absence of test drugs. Phosphatidylinositol turnover was quantified by ion exchange chromatography and cytokine contentofsupernatantsbyenzyme-linkedimmunosorbentassay.Results: Antazoline hydrochloride, emedastine difumarate, levocabastine hydrochloride, olopatadine hydrochloride, and pheniramine maleate attenuated histaminestimulated phosphatidylinositol turnover and IL-6 and IL-8 secretion. Emedastine was the most potent in ligand binding, phosphatidylinositol turnover, and IL-6 secretion, with dissociation constant and 50% inhibitory concentrations of 1-3 nmol/L. Olopatadine, antazoline, and pheniramine exhibited similar H 1 -binding affinities (32-39 nmol/L). However, olopatadine was approximately 10-fold more potent as an inhibitor of cytokine secretion (50% inhibitory concentration, 1.7-5.5 nmol/L) than predicted from binding data, while antazoline and pheniramine were far less potent (20-to 140-fold) in functional assays. Levocabastine (dissociation constant, 52.6 nmol/L) exhibited greater functional activity (50% inhibitory concentration, 8-25 nmol/L) than either antazoline or pheniramine.Conclusions: Histamine-stimulated phosphatidylinositol turnover and cytokine secretion by human conjunctival epithelial cells are attenuated by compounds with H 1 -antagonist activity. However, antihistaminic potency alone does not predict anti-inflammatory potential. Olopatadine, emedastine, and levocabastine were notably more potent than pheniramine and antazoline.Clinical Relevance: Selected topical ocular drugs with antihistaminic activity may offer therapeutic advantages to patients with allergic conjunctivitis by inhibiting proinflammatory cytokine secretion from human conjunctival epithelial cells.

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Section: Commentmentioning

confidence: 99%

Inhibition of Histamine-Induced Human Conjunctival Epithelial Cell Responses by Ocular Allergy Drugs

Yanni

Weimer²,

Sharif³

et al. 1999

Arch Ophthalmol

100

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“…Recently, cetirizine and loratadine were compared for their effects on GM-CSF and IL-8 production by human bronchial epithelial cells in vitro. Neither drug had any effect on cytokine production by the epithelial cells [43].…”

Section: Cetirizine -Effects On Epithelial Cells and Endothelial Cellsmentioning

confidence: 83%

The effects of cetirizine on the function of inflammatory cells involved in the allergic response

Walsh

1997

Clin Experimental Allergy

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“…Another study compared cetirizine and loratadine for their effects on GM‐CSF and IL‐8 production by human bronchial epithelial cells in vitro . Neither drug had any effect on cytokine production by the epithelial cells (11). So it seems that the in vivo situation is much more complex than it appears at first examination – I think these examples do emphasize the difficulty in dissecting the mechanism of action of the anti‐inflammatory effects of drugs like cetirizine.…”

mentioning

confidence: 95%

General discussion – Round‐table meeting “The therapeutic index of antihistamines” Amsterdam, 5–6 May 1998

2000

Allergy

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Antihistamines and production of granulocyte-macrophage colony-stimulating factor and interleukin-8 by human bronchial epithelial cells in vitro: Evaluation of the effects of loratadine and cetirizine

Cited by 7 publications

References 20 publications

Inhibition of Histamine-Induced Human Conjunctival Epithelial Cell Responses by Ocular Allergy Drugs

Inhibition of Histamine-Induced Human Conjunctival Epithelial Cell Responses by Ocular Allergy Drugs

The effects of cetirizine on the function of inflammatory cells involved in the allergic response

General discussion – Round‐table meeting “The therapeutic index of antihistamines” Amsterdam, 5–6 May 1998

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Antihistamines and production of granulocyte-macrophage colony-stimulating factor and interleukin-8 by human bronchial epithelial cells in vitro: Evaluation of the effects of loratadine and cetirizine

Cited by 7 publications

References 20 publications

Inhibition of Histamine-Induced Human Conjunctival Epithelial Cell Responses by Ocular Allergy Drugs

Inhibition of Histamine-Induced Human Conjunctival Epithelial Cell Responses by Ocular Allergy Drugs

The effects of cetirizine on the function of inflammatory cells involved in the allergic response

General discussion – Round‐table meeting “The therapeutic index of antihistamines” Amsterdam, 5–6 May 1998

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General discussion – Round‐table meeting “The therapeutic index of antihistamines” Amsterdam, 5–6 May 1998