Antihyperglycemic and hypoglycemic activity of naturally occurring peptides and protein hydrolysates from easy-to-cook and hard-to-cook beans (Phaseolus vulgaris L.)

Valencia-Mejía, Erika; Batista, Karla de Aleluia; Acevedo-Fernández, Juan José; Fernandes, Kátia Flávia

doi:10.1016/j.foodres.2019.03.043

Cited by 59 publications

(58 citation statements)

References 39 publications

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“…Therefore, HTC beans are usually disposed of and as a consequence, there is a large amount of non-commercially viable residues. However, this amount of disposable beans may still be used as a source of nutraceutical components (6,7), thus avoiding food wasting and obtaining some therapeutic benefits.…”

Section: Introductionmentioning

confidence: 99%

“…Several biological effects of protein extracts produced from beans have been described, such as: antihyperglycemic (7), anticancer (8), ACE inhibitory and potentially antihypertensive (9), anti-inflammatory (10), and antioxidant (11). Notwithstanding the aforementioned results obtained in non-HTC, it is worth hypothesizing that HTC beans may also serve as a source of bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

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Oxidonitrergic and antioxidant effects of a low molecular weight peptide fraction from hardened bean (Phaseolus vulgaris) on endothelium

Graziani

Ribeiro

Cruz

et al. 2021

Braz J Med Biol Res

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About 3000 tons of beans are not used in human food due to hardening. Several studies on bean-derived bioactive peptides have shown potential to treat some diseases, including those relying on oxidative dysfunctions. We assessed the effects of peptides extracted from hardened bean Phaseolus vulgaris (PV) on reactive oxygen species (ROS) and nitric oxide (NO) production, cytotoxic and cytoprotective effects in endothelial cells, and oxidonitrergic-dependent vasodilating effects. Extract was composed by peptide fraction <3 kDa (PV3) from hardened common bean residue. PV3 sequences were obtained and analyzed with bioinformatics. Human umbilical vein endothelial cells were treated with 10, 20, 30, and 250 µg/mL PV3. Oxidative stress was provoked by 3% H 2 O 2 . Cytotoxicity and cytoprotective effects were evaluated by MTT assay, whereas, ROS and NO were quantified using DHE and DAF-FM fluorescent probes by confocal microscopy. NO- and endothelium-dependent vasodilating effects of PV3 were assessed in isolated aortic rings. We found 35 peptides with an average mass of 1.14 kDa. There were no cell deaths with 10 and 20 μg/mL PV3. PV3 at 30 μg/mL increased cell viability, while cytotoxicity was observed only with 250 μg/mL PV3. PV3 at 10 μg/mL was able to protect cells from oxidative stress. PV3 also increased NO release without causing cell death. It also reduced relative ROS production induced by H 2 O 2 . PV3 vasodilating effects relied on endothelium-dependent NO release. PV3 obtained from low-commercial-value bean displays little cytotoxicity and exerts antioxidant effects, whereas it increases endothelial NO release.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oxidonitrergic and antioxidant effects of a low molecular weight peptide fraction from hardened bean (Phaseolus vulgaris) on endothelium

Graziani

Ribeiro

Cruz

et al. 2021

Braz J Med Biol Res

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“…This is because α-amylase and α-glucosidase are enzymes that digest carbohydrates to release glucose to cause blood sugar elevation in non-insulin-dependent diabetes mellitus, obesity, and hyperglycemia (Cetto, Wiedenfeld, Revilla, & Sergio, 2000). For example, the α-amylase and α-glucosidase inhibitory activities of enzymatic protein hydrolyzates produced from pea (Awosika & Aluko, 2019), beans (Valencia-Mejía, Karla, Fernández, & Fernandes, 2019), and mushroom (Zhang et al, 2019) have been reported. Since some of these peptides have been known to possess multifunctional properties, they could serve not only as α-amylase and α-glucosidase inhibitors, but also as antioxidants and antihypertensive agents (Arise et al, 2016;Zhang et al, 2019).…”

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confidence: 99%

Antioxidant and enzymes inhibitory properties of Amaranth leaf protein hydrolyzates and ultrafiltration peptide fractions

et al. 2020

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Food proteins have gained increasing value in recent times because of the rapidly expanding research and knowledge based on the contents of encrypted physiologically active amino acid sequences (Miguel, Alonso, Salaices, Aleixandre, & López-Fandiño, 2011). Proteins from food sources usually contain specific peptide sequences that remain inactive when present as intrinsic parts of the primary protein structure and one of the most reliable ways to release these peptides is through enzymatic hydrolysis of parent protein (Aluko, 2015).

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“…Mochida et al [233] reported that zein-derived peptides induced glycemic regulation via GLP-1 secretion, and DPP-IV inhibition in rats, whereas Ishikawa et al [234] obtained similar results by employing rice-derived peptides. Valencia-Mejía et al [109] studied the antihyperglycemic and hypoglycemic activity of naturally occurring peptides and protein hydrolysates from beans in male Wistar rats.…”

Section: Vertebrates Modelsmentioning

confidence: 99%

Antidiabetic Food-Derived Peptides for Functional Feeding: Production, Functionality and In Vivo Evidences

Rivero‐Pino

Espejo-Carpio

Guadix

2020

Foods

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Bioactive peptides released from the enzymatic hydrolysis of food proteins are currently a trending topic in the scientific community. Their potential as antidiabetic agents, by regulating the glycemic index, and thus to be employed in food formulation, is one of the most important functions of these peptides. In this review, we aimed to summarize the whole process that must be considered when talking about including these molecules as a bioactive ingredient. In this regard, at first, the production, purification and identification of bioactive peptides is summed up. The detailed metabolic pathways described included carbohydrate hydrolases (glucosidase and amylase) and dipeptidyl-peptidase IV inhibition, due to their importance in the food-derived peptides research field. Then, their characterization, concerning bioavailability in vitro and in situ, stability and functionality in food matrices, and ultimately, the in vivo evidence (from invertebrate animals to humans), was described. The future applicability that these molecules have due to their biological potential as functional ingredients makes them an important field of research, which could help the world population avoid suffering from several diseases, such as diabetes.

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Antihyperglycemic and hypoglycemic activity of naturally occurring peptides and protein hydrolysates from easy-to-cook and hard-to-cook beans (Phaseolus vulgaris L.)

Cited by 59 publications

References 39 publications

Oxidonitrergic and antioxidant effects of a low molecular weight peptide fraction from hardened bean (Phaseolus vulgaris) on endothelium

Oxidonitrergic and antioxidant effects of a low molecular weight peptide fraction from hardened bean (Phaseolus vulgaris) on endothelium

Antioxidant and enzymes inhibitory properties of Amaranth leaf protein hydrolyzates and ultrafiltration peptide fractions

Antidiabetic Food-Derived Peptides for Functional Feeding: Production, Functionality and In Vivo Evidences

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