Antihyperglycemic Property ofTragia cannabinain Streptozotocin-Induced Diabetic Rats

Abstract

The present study was performed to investigate the efficacy of an ethanol extract of the roots of Tragia cannabina for antihyperglycemic and antioxidant effects in streptozotocin (STZ)-induced diabetic rats. Male Wistar rats were administered T. cannabina (250 mg/kg) orally for 21 days, and blood glucose level was measured weekly. At the end of 21 days, concentrations of serum lipids such as total cholesterol, triglycerides, and high-density lipoprotein (HDL) and protein markers such as total protein, albumin, globulin, and albumin:globulin ratio (A:G) were estimated. Also, levels of enzymes such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), and alkaline phosphatase (ALP) were determined. Antioxidant activity of the extract was evaluated by estimating lipid peroxides (LPO), superoxide dismutase (SOD), and catalase in liver of normal control and STZ- and extract-treated rats. Histopathological changes of liver and kidney were also studied in STZ-induced diabetic animals and normal controls. All these effects produced by the extract were compared with glibenclamide, a standard antidiabetic drug. Oral administration of T. cannabina for 21 days resulted in a significant reduction in blood glucose level, lipid concentration, and SGOT, SGPT, ALP, and LPO levels accompanied by an increase in the levels of SOD and catalase in liver tissues of STZ-induced diabetic rats. Altered levels of protein markers also reverted back to normal. Histopathological changes of liver and kidney were returned to normal. The effects produced by the extract were comparable to that of glibenclamide. In conclusion, the T. cannabina showed significant antihyperglycemic and antioxidant effects in STZ-induced diabetic rats.

Antidiabetic and Antioxidant Activities of Ethanolic Extract of Dried Flowers of Moringa oleifera in Streptozotocin-induced Diabetic Rats

“…Elevated levels of aminotransferases in the serum are a common sign of liver and kidney damage and are observed more often among diabetics than in the general population (43). This study shows that the levels of liver aminotransferase (ALT and AST) increased significantly in STZ-induced diabetic untreated rats as shown in Tables 7 and 8, respectively.…”

Phytochemical, Pharmacological and Biological Profiles of Tragia Species (Family: Euphorbiaceae)

“…Ethanolic extract of T. cannabina leaf at an oral dose of 250 mg/kg, showed remarkable antihyperglycemic and antioxidant effects in STZ-induced diabetic rats. This study also confirmed the nontoxic properties of the ethanolic extract of T. cannabina leaf (Sivajothi et al, 2007). Moreover, the antidiabetic effect of an isolated phytocompound, 4-oxo-4H-pyran-2,6-dicarboxylic acid-bis-[6-methyl-heptyl] ester from T. cannabina have been demonstrated using in vitro streptozotocin (STZ) -treated chick embryo and in silico docking studies targeting active sites of AMPK (Sivajothi and Dakappa, 2014).…”

Altered Kidney Morphology and Enzymes in Streptozotocin Induced Diabetic Rats

“…The present study was designed to observe the altered morphology of kidney and kidney enzyme levels in STZ-induced diabetes at various time intervals and to investigate a possible association between diabetic nephropathy and kidney enzymes alterations. Elevated activities of serum aminotransferases are a common sign of kidney damage and are observed more frequently among people with diabetes than in general population (Fadillioglu et al;Sivajothi et al, 2007;AlShamsi et al, 2006;McAnuff-Harding et al, 2006;Ohaeri, 2001). …”