Antihyperlipidemic effect of hydroalcoholic extract, and polyphenolic fraction from Dracocephalum kotschyi Boiss

Objective: The aim of this study was to evaluate the anti-inflammatory and analgesic effects of Satureja hortensis seed extracts and to analyze their essential oil constituents. Materials and Methods: Hydroalcoholic and polyphenolic extracts and essential oil of S. hortensis seeds were prepared using standard methods. Analgesic activity was assessed in male mice (25-35 g) using standard methods (acetic acid and formalin tests). For the evaluation of anti-inflammatory activity, the carrageenan-induced rat paw edema test was used. The mice were pretreated with 50, 100 or 200 mg/kg, i.p., hydroalcoholic or polyphenolic extracts or 100 or 200 µl/kg, p.o. Results: Hydrodistillation of the seeds of S. hortensis afforded a pale yellowish oil in a yield of 0.05% (v/w). Pretreatment of mice with hydroalcoholic or polyphenolic extracts or essential oil significantly (p < 0.001) reduced acetic acid-induced abdominal twitches. Hydroalcoholic extracts also significantly reduced pain responses in early and late phases of the formalin test whereas the polyphenolic extract and essential oil were only effective in the late phase of the formalin test. All three fractions were found to reduce paw edema in the carrageenan test. Conclusion: These results clearly demonstrate the analgesic and anti-inflammatory activity of S. hortensis seeds and since the hydroalcoholic extract relieved pain in the first phase of the formalin test, it seems that at least part of its analgesic activity may be mediated centrally. The results of this study substantiated the traditional use of S. hortensis plant seeds in painful and inflammatory ailments.

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“…The extract was then shaken, filtered and freeze-dried. The yield (percent of extract weight to initial weight of seed powder) was 9.5% [13]. …”

Section: Methodsmentioning

confidence: 99%

“…The combined extracts were evaporated to about one third of their original volume and finally extracted with chloroform in a separatory funnel. The resulting aqueous solution was freeze-dried and its yield (percent of extract weight to initial weight of seed powder) was 4.5% [13]. …”

Section: Methodsmentioning

confidence: 99%

Antinociceptive and Anti-Inflammatory Activities of <i>Satureja hortensis</i> Seed Essential Oil, Hydroalcoholic and Polyphenolic Extracts in Animal Models

2011

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“…Its leaf extract has been reported to have antihyperlipidemic (Ebrahim Sajjadi et al 1998), immunomodulatory (Amirghofran et al 2000), antinociceptive (Golshani et al 2004) and cytotoxic (Jahaniani et al 2005) effects. Aerial parts of D. kotschyi plants are sources of valuable flavonoids and essential oils (Ebrahim Sajjadi et al 1998;Gohari et al 2003;Monsef-Esfahani et al 2007;Saeidnia et al 2007) and its seeds are rich in linolenic, oleic and linoleic acids (Goli et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Overproduction of valuable methoxylated flavones in induced tetraploid plants of Dracocephalum kotschyi Boiss

Zahedi¹,

Hosseini²,

Fattahi³

et al. 2014

Planta Med

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“…To prepare hydroalcoholic extract, powdered plant (100 g) was macerated by 1500 ml of ethanol 70% (v/v) for 72 h. The extract was then shaken, filtered and the solvent was removed in a vacuum evaporator to obtain semi-solid extract and then it was placed in an oven in 60°C for 72 h (10). …”

Section: Methodsmentioning

confidence: 99%

Antinociceptive Effect of Hydroalcoholic Extract of Iranian Green tea in the Formalin Test in Rats

Arzi

Ghorbanzadeh²,

Khorasgani³

2013

Jundishapur J Nat Pharm Prod

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Background: Tea (Camellia sinensis) has been utilised, since time immemorial, as a beverage possessing encouraging health benefits. Little scientific evidence exists in literature on the effect of this plant on pain. Objectives: To investigate the antinociceptive activity of Iranian green tea extract. Materials and Methods:The hydroalcoholic extract was administered to male Wistar rats. Formalin paw test was used to evaluate the antinociceptive activity. Plant extract (25, 50, 100 and 200 mg/kg, i.p.) (n = 6 for each group) or vehicle (n = 6) was administered 30 min before the subplantar formalin injection. Results: The extract caused a significant dose-related (50, 100, 200 mg /kg, i.p.) inhibition of the first phase and onset of chronic phase (200 mg /kg, i.p.) of formalin induced nociception. The results showed that the pre-treatment of rats with naloxone (1 mg/kg, i.p.) significantly (P < 0.001) reversed antinociception by Green tea extract (GTE) (200 mg/kg, i.p.) in the inflammatory phase and had no effect on phase 1. Conclusions: These results indicate that GTE produces dose-related antinociception in chemical pain model and one of its possible mechanisms involves opioid pathways.

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Antihyperlipidemic effect of hydroalcoholic extract, and polyphenolic fraction from Dracocephalum kotschyi Boiss

Cited by 69 publications

References 3 publications

Antinociceptive and Anti-Inflammatory Activities of <i>Satureja hortensis</i> Seed Essential Oil, Hydroalcoholic and Polyphenolic Extracts in Animal Models

Antinociceptive and Anti-Inflammatory Activities of <i>Satureja hortensis</i> Seed Essential Oil, Hydroalcoholic and Polyphenolic Extracts in Animal Models

Overproduction of valuable methoxylated flavones in induced tetraploid plants of Dracocephalum kotschyi Boiss

Antinociceptive Effect of Hydroalcoholic Extract of Iranian Green tea in the Formalin Test in Rats

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