Antihypernociceptive and Neuroprotective Effects of the Aqueous and Methanol Stem-Bark Extracts of Nauclea pobeguinii (Rubiaceae) on STZ-Induced Diabetic Neuropathic Pain

Abstract

The greatest common and devastating complication of diabetes is painful neuropathy that can cause hyperalgesia and allodynia. It can disturb psychosocial functioning by increasing levels of anxiety and depression. This work was designed to evaluate the antihyperalgesic, antidepressant, and anxiolytic-like effects of the aqueous and methanol extracts of Nauclea pobeguinii stem-bark in diabetic neuropathy induced by streptozotocin in mice. Diabetic neuropathy was induced in mice by the intraperitoneal administration of 200 mg/kg streptozotocin (STZ) to provoke hyperglycemia. Nauclea pobeguinii aqueous and methanol extracts at the doses of 150 and 300 mg/kg were administered by oral route, and their effects were evaluated on antihyperalgesic activity (Von Frey filaments, hot plate, acetone, and formalin tests), blood glucose levels, body weight, serum, sciatic nerve proinflammatory cytokines (TNF-α, IL-1β, and IL-6) and sciatic nerve growth factor (IGF and NGF) rates, depression (open field test, forced swimming test, tail suspension test), and anxiety (elevated plus maze, light-dark box test, social interaction). Oral administration of Nauclea pobeguinii stem-bark aqueous and methanol extracts (150 and 300 mg/kg) produced antihyperalgesic, antidepressant, and anxiolytic-like effects in STZ-induced diabetic neuropathic mice. Extracts also triggered a decrease in glycaemia and increased body weight in treated animals. They also significantly ( p <0.001) reduced tumour necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and IL-6 and significantly ( p <0.001) increased nerve growth factor (NGF) and insulin-like growth factor (IGF) in sciatic nerves. The results of this study confirmed that Nauclea pobeguinii aqueous and methanol extracts possess antihyperalgesic, antidepressant, and anxiolytic activities and could be beneficial therapeutic agents.

Securidaca inappendiculata Polyphenol Rich Extract Counteracts Cognitive Deficits, Neuropathy, Neuroinflammation and Oxidative Stress in Diabetic Encephalopathic Rats via p38 MAPK/Nrf2/HO-1 Pathways

“…A behavioural depressive state is indicated by increase in immobility time displayed by experimental animals in the OFT and FST tests ( Shalam et al, 2007 ). In line with previous studies, we observed that diabetic rats exhibited depressive like behaviour as indicated by increased immobility time in the FST, as well as reduced locomotive activity in the OFT test when compared to the normoglycemic rats ( de Morais et al, 2014 , de Morais et al, 2016 ; Tsafack et al, 2021 ). SiPE administration significantly reduced the immobility time and increased the swimming time as well as the exploratory behaviour in the FST and OFT, respectively.…”

“…In fact diabetes and depression have been shown to display a bifacial etiology, as patients suffering from depression have a higher risk of developing T2DM due to the activation of several pathways that can initiate insulin resistance (Champaneri et al, 2010;Deischinger et al, 2020). In addition, previous studies have illustrated that anxiety and depression are characteristic features associated with diabetic neuropathy (Mbiantcha et al, 2019;Tsafack et al, 2021). FST and OFT are the most commonly used behavioural studies for evaluating antidepressant activity of pharmacological agents (Dulawa et al, 2004;Cryan et al, 2005).…”

“…The forced swimming test was accessed based on previously reported method ( Mu et al, 2020 ; Tsafack et al, 2021 ). The swimming apparatus consisted of a transparent cylinder shaped container (30 cm × 45 cm) filled with water at 25°C to a depth of 25 cm.…”

Isolation and Identification of β-sitosterol, 7-hydroxystigmast-22-en-3,6-dione and 3β, 24(S)-dihydroxycholesta-5, 25-diene-7-one from stem bark of Nauclea pobeguinii

“…It has reportedly been useful in the treatment of jaundice, gonorrhea, fever, stomach ache, and epilepsy (Njoya et al, 2017). In Cameroon, the plant is used in the management of hyperglycemia, articular pain, stomach pains, and inflammation (Tsafack et al, 2021). Anti-helminthic application of stem bark water decoction of N. diderrichi and N. pobeguinii were also reported in Congo while its anti-malaria activity was recorded in Mali (Haudecoeur et al, 2018).…”