Antihypertensive Activities of Peptides Derived from Porcine Skeletal Muscle Myosin in Spontaneously Hypertensive Rats

Abstract

Antihypertensive activities derived from porcine skeletal muscle proteins were investigated. Thermolysin hydrolysates of porcine muscle water-insoluble proteins demonstrated antihypertensive activities in spontaneously hypertensive rats when administrated in single oral doses. Hydrolysates of porcine myosin and peptides (Met-Asn-Pro-Pro-Lys, Ile-Thr-Thr-Asn-Pro, Met-Asn-Pro, Pro-Pro-Lys) with parts of the sequence of myosin showed antihypertensive activities. This is the first report of antihypertensive activities of peptides derived from muscle proteins of domestic animals. The hydrolysates of porcine muscle protein and their corresponding bioactive peptides might be utilized for physiologically functional foods.

Effects of Dietary Oyster Extract on Lipid Metabolism, Blood Pressure, and Blood Glucose in SD Rats, Hypertensive Rats, and Diabetic Rats

“…That is, these weak inhibitory peptides might be converted into strong ones by gastrointestinal proteases which are absorbed in intact form to exhibit antihypertensive effects. [46][47][48] Peptides in oyster extract might serve as prodrug-type ACE inhibitory peptides. Further investigation is necessary on this point.…”

Peptides: Production, bioactivity, functionality, and applications

“…These peptides are very valuable because they have variety of functions and they are easily absorbed in the body and therefore, they can potentially be considered a great alternative for the synthetic antihypertensive drugs (Lee et al, 2010). Although a huge body of research has been dedicated to antihypertensive effect of bioactive peptides from plant (Motoi and Kodama, 2003;Chen et al, 2003;Motoi and Kodama, 2003;Nakano et al, 2006;Kodera and Nio, 2006;Kodera and Nio, 2006;Lee et al, 2006a, b;Zhu et al, 2006;OsegueraToledo et al, 2011;Gonz alez-Garc ıa et al, 2014;OsegueraToledo et al, 2015;Capriotti et al, 2015;Esteve et al, 2015;Coscueta et al, 2016;Da Silva Vaz et al, 2016), terrestrial animals (Morimatsu et al, 1996;Arihara et al, 2001;Nakashima et al, 2002;Saiga et al, 2003;Vercruysse et al, 2005;Arihara, 2006;Yu et al, 2006;Sentandreu and Toldra, 2007;Li et al, 2007;Chang et al, 2007;Wang et al, 2008a;Xu et al, 2009;Shimizu et al, 2009;Kim et al, 2009;Liu et al, 2010;Bernardini et al, 2012;Escudero et al, 2013), seafood (Jeon et al, 1999;Byun and Kim, 2001;Morimura et al, 2002;…”

Biologically Active Peptides Released From Food Proteins

“…W hydrolizatach enzymatycznych miozyny wieprzowej (łańcuch ciężki) zidentyfikowano miopentapeptydy A (MNPPK) oraz B (ITTNP). Karmienie szczurów dawką 1 mg tych peptydów na kilogram masy ciała powodowało obniżenie skurczowego ciśnienia krwi odpowiednio o 23,4 oraz 21,0 mm Hg [72]. Źródłem inhibitorów ACE jest wędzona szynka hiszpańska.…”