Antihypertensive Effect of Ethanolic Extract from Acanthopanax sessiliflorus Fruits and Quality Control of Active Compounds

Abstract

Acanthopanax sessiliflorus (Rupr. & Maxim.) Seem., which belongs to the Araliaceae family, mainly inhabits Korea, China, and Japan. Traditionally, Acanthopanax species have been used as treatment for several diseases such as diabetes, tumors, and rheumatoid arthritis. Especially, its fruits have many biological functions including antitumor, immunostimulating, antithrombosis, and antiplatelet activities. Recently, the extract of A. sessiliflorus fruit has been reported to have antithrombotic and antiplatelet activities related to the alleviation of hypertension. Therefore, we investigated the antihypertensive effect of ethanolic extract from A. sessiliflorus fruits (DHP1501) through in vivo, ex vivo, and in vitro studies. In this study, DHP1501 demonstrated free radical scavenging capacity, enhanced endothelial nitric oxide (NO) production, and inhibited angiotensin-converting enzyme (ACE) activity in spontaneously hypertensive rats (SHRs), resulting in the improvement of vascular relaxation and decrease in blood pressure in the hypertensive animal model. These results suggest that A. sessiliflorus fruit extract may be a promising functional material for the prevention and treatment of hypertension. Furthermore, this study demonstrated the utility of MS-based active compounds for the quality control of DHP1501.

Acanthopanax senticosus Induces Vasorelaxation via Endothelial Nitric Oxide-Dependent and -Independent Pathways

“…These findings suggest that the increase observed in intracellular NO production via elevated phospho-endothelial NO synthase is the underlying mechanism for this occurrence. The ethanolic extract from Acanthopanax sessiliflorus (Araliaceae) fruits has also been reported to enhance endothelial NO production in cultured human umbilical vein endothelial cells and decrease blood pressure in SHR [16]. The increased phosphorylation of endothelial NO synthase in the presence of ASRE has been reported in cultured lymphatic endothelial cells and was also found to ameliorate lower limb edema in healthy volunteers treated with ASRE [17].…”

“…*p < 0.05. effects during a hypertensive state. The ethanolic extract from A. sessiliflorus fruits has been reported to inhibit angiotensin converting enzyme activity, leading to decreased blood pressure in SHR [16]. Administration of the extract (600 mg/kg/d) for 4 wk decreased the blood pressure to levels comparable to those after captopril administration (100 mg/kg/d).…”

Development of a standardized combined plant extract containing nutraceutical formulation ameliorating metabolic syndrome components

“…Systolic and diastolic arterial blood pressure of experimental animals was measured by tail-cuff method [47][48][49].…”

“…After anaesthetizing the rats, blood samples were collected from the inferior venacava, kept for 15-20 min at room temperature and then centrifuged at 3000 rpm; the separated serum stored at − 80 °C and rennin and ACE concentrations measured at ng/mL using ELISA kits [47][48][49].…”

“…Relaxation was expressed as a percentage change from KCl contracted levels i.e., by comparison between maximum vascular contraction before and after addition of test sample (PNC) [47][48][49][50].…”