Antihypertensive Effect of Peptides from Royal Jelly in Spontaneously Hypertensive Rats

Tokunaga, Katsuhiko; Yoshida, Chie; Suzuki, Kunihiro; Maruyama, Haruhiko; Futamura, Yoshihiro; Araki, Yasufumi; Mishima, Satoshi

doi:10.1248/bpb.27.189

Cited by 136 publications

(97 citation statements)

References 12 publications

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“…Commercial RJ is obtained by harvesting the brood food from the queen cell in the 3rd day of larval development (ISO 12824:2016). (Fujii et al, 1990;Kohno et al, 2004), immunoregulatory (Okamoto et al, 2003;Vucevic et al, 2007), insulin-like (Dixit and Patel, 1964;Kramer et al, 1982), estrogen signaling mediating (Moutsatsou et al, 2010), antiaging (Inoue et al, 2003), reducing cholesterol levels (Kashima et al, 2014), vasodilation (Tokunaga et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Comparative Study on Quality Parameters of Royal Jelly, Apilarnil and Queen Bee Larvae Triturate

Mărgăoan¹,

Mărghitaș²,

Dezmirean³

et al. 2017

BUASVMCN-ASB

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Given their beneficial effects in terms of health, the natural products, especially beehive products, have drawn the attention of consumers since long time ago. In order to guarantee the quality of these products on the market, their chemical composition needs to be analyzed. Thus, this current research had as objective the establishment of quality parameters for beehive brood food derived products: apilarnil and queen bee larvae triturate. These two products were compared with royal jelly which is the basis of brood food in the first 3 days of larval stage. The carbohydrates were determined by HPLC-IR and allowed the identification of seven carbohydrate compounds, predominantly glucose, fructose and sucrose. The lipid profile was analyzed by the Soxhlet method. The total protein content was determined by the Kjeldahl method. Free amino acids were analyzed by LC-MS. A total of 31 amino acids were identified of which nine are essential amino acids for humans.

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Section: Introductionmentioning

confidence: 99%

Comparative Study on Quality Parameters of Royal Jelly, Apilarnil and Queen Bee Larvae Triturate

Mărgăoan¹,

Mărghitaș²,

Dezmirean³

et al. 2017

BUASVMCN-ASB

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“…Previous studies have demonstrated a wide range of health benefits to humans and model organisms, including vasodilative and hypotensive activities (Tokunaga et al, 2004), antitumor (Tamura et al, 1987), anti-inflammatory (Fujii et al, 1990;Kohno et al, 2004), antibacterial and disinfectant properties (Fujiwara et al, 1990;Shen et al, 2010a), antifatigue activity (Kamakura et al, 2001a), and increasing the average lifespan in mice (Inoue et al, 2003) and nematodes (Honda et al, 2011). Because of its great potential benefits to human health, RJ is widely used as a key component in many commercial products including medicine, dietary supplements, and cosmetics (Kamakura et al, 2001c).…”

Section: Introductionmentioning

confidence: 99%

Determination of royal jelly freshness by ELISA with a highly specific anti-apalbumin 1, major royal jelly protein 1 antibody

Shen

Wang

Zhai

et al. 2015

J. Zhejiang Univ. Sci. B

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Major royal jelly protein 1 (MRJP1), designated apalbumin 1, has been regarded as a freshness marker of royal jelly (RJ). A MRJP1-specific peptide (IKEALPHVPIFD) identified by bioinformatics analysis of homologous members of the major royal protein family was synthesized and used to raise polyclonal anti-MRJP1 antibody (anti-SP-MRJP1 antibody). Western blot analysis showed that anti-SP-MRJP1 antibody only reacted with MRJP1 in RJ. In contrast, the previously reported antibody against recombinant MRJP1 (anti-R-MRJP1 antibody) reacted with other members of MRJP family in RJ. Enzyme-linked immunosorbent assay (ELISA) using anti-SP-MRJP1 antibody demonstrated that MRJP1 content in RJ stored at 40 °C significantly degraded by 37.3%, 55.9%, 58.0%, 60.6%, 65.7%, 72.7%, and 73.1% at 7, 14, 21, 28, 35, 42, and 49 d, respectively, when compared with MRJP1 content in fresh RJ (0 d). Optical density analysis of MRJP bands from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles demonstrated that the degradation of MRJP1, MRJP2, MRJP3, and MRJP5 in RJ was strongly and positively correlated with the period of storage (P<0.0001). Our results indicated anti-SP-MRJP1 antibody was highly specific for MRJP1, and ELISA using the antibody is a sensitive and easy-to-use method to determine the freshness and authenticity of RJ.

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“…Hypotensive effects of ACE-inhibitory peptides from royal jelly (Tokunaga et al, 2004), egg white hydrolyzate (Miguel et al, 2005), and jellyfish (Morinaga et al, 2010) were observed in SHR following a single oral administration. The 2 peptides isolated from A. americana tuber hydrolyzate in the present study showed hypotensive effects in SHR equal to or greater than those isolated previously.…”

Section: Fig 1 Hplc Chromatogram Of Peptides In the S2 Fraction Shmentioning

confidence: 98%

Angiotensin-Converting Enzyme-Inhibitory Peptides Isolated from Pepsin Hydrolyzate of Apios americana Tuber and Their Hypotensive Effects in Spontaneously Hypertensive Rats

Kuramoto

Kaneyoshi

Morinaga

et al. 2013

FSTR

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With the aim of elucidating the hypotensive effects of Apios americana Medikus tuber, active ingredients with angiotensin-converting enzyme (ACE)-inhibitory activity were identified and their hypotensive effects in spontaneously hypertensive rats (SHR) were investigated. From a pepsin digest of A. americana showing strong activity, two ACE-inhibitory peptides were isolated and identified as YRLPNL and YQLP. Although the ACE-inhibitory activity of YQLP was weak, YRLPNL showed strong ACE-inhibitory activity (IC 50 value 7.7 nM) equal to those of lisinopril and captopril. After a single oral administration of YRLPNL, SHR showed a significant decrease in blood pressure. These results suggest that YRLPNL, isolated from A. Americana, is a hypotensive peptide having ACE-inhibitory activity. This is the first report of a food peptide having ACE-inhibitory activity equal to that of pharmaceuticals.

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Antihypertensive Effect of Peptides from Royal Jelly in Spontaneously Hypertensive Rats

Cited by 136 publications

References 12 publications

Comparative Study on Quality Parameters of Royal Jelly, Apilarnil and Queen Bee Larvae Triturate

Comparative Study on Quality Parameters of Royal Jelly, Apilarnil and Queen Bee Larvae Triturate

Determination of royal jelly freshness by ELISA with a highly specific anti-apalbumin 1, major royal jelly protein 1 antibody

Angiotensin-Converting Enzyme-Inhibitory Peptides Isolated from Pepsin Hydrolyzate of Apios americana Tuber and Their Hypotensive Effects in Spontaneously Hypertensive Rats

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